Research Profile

Mary McCaffrey

Biography

My B.Sc. was in the Biological Sciences, specialising in Microbiology (B.Sc. 1H, , UCG, 1983).  My Doctoral studies were done in London, studying retroviral insertional mutagenesis in the search for genes involved in causing cancer (oncogenes) at the Imperial Cancer Research Funds (now CRUK, The London Institute), Lincoln's Inn Fields, London, obtaining a Ph.D from the University of London (UCL) in 1989.

Two periods (four years in total) of post-Doctoral training followed in France; firstly with Pascal Madaule, in the CNRS laboratories, Gif-sur-Yvette on Rho GTPases and then, with Bruno Goud, in the Pasteur Institute, Paris, working on Rab GTPases.  

I established my laboratory in Cork in the early 1990s and since then I have successively been a College Lecturer, Senior Lecturer and now Associate Professor of Biochemistry in UCC.

For further details on the laboratory see Mary's Lab Page.

Also, since the early 1990s, I have been a member of the Biochemical Society, holding the following positions in the Society:

  • Council Member: 2015 –
  • Cell Biology Panel Chair: 2011 - 2014
  • Cell Biology Panel Vice-Chair: 2008 - 2010
  • Member of Cell Biology Panel (TPIV): 2004 - 2007

For further information on the Biochemical Society see www.biochemistry.org.

Research Interests

The monomeric Rab GTPases are important regulatory proteins which control eukaryotic intracellular trafficking events of both the secretory and endocytic pathway. The Rab11 subfamily (Rab11a, Rab11b and Rab25) are critical regulators of endosomal recycling have been shown to function in several crucial cell physiological processes such as cell division/cytokinesis, cell migration, insulin response events, and memory processes (long term potentiation - LTP). Furthermore, Rab11 GTPases have been implicated in disease conditions ranging from cancer to Alzheimer’s disease.

My group concentrates on the functional characterisation of events that occur downstream of the Rab11 GTPases, primarily by the identification and characterisation of novel proteins which interact with the GTP-bound Rab and ‘effect’ or ‘elicit’ its function downstream. The major group of Rab11 effector proteins, so far identified, is the Rab11 Family Interacting Proteins (FIPs) which we, and others, discovered about fifteen years ago. The FIPs comprise of the Rab Coupling Protein (RCP), Rip11, FIP2, FIP3 and FIP4. Individual FIP protein function is now known to be central to a rapidly increasing range of crucial cellular processes involving endosomal trafficking/recycling - with Rip11 underpinning cellular GLUT4 plasma membrane translocation response to insulin; RCP function crucial for α5ß1 integrin and EGF-R recycling and cell motility; FIP2 for calcium-induced AMPA-R recycling at the post-synaptic dendritic spines and thus long-term potentiation; FIP3 for cytokinesis and endosomal-recycling compartment (ERC) homeostasis and FIP4 for retinal cell development. For further details of our contribution to the current understanding of FIP function see the relevant publications listed below.

My group has trained a significant number of graduates in Biochemistry and Cell Biology. Nine Ph.Ds. and four M.Scs. have graduated from the laboratory during the past two decades, along with approximately 100 final year B.Sc. student and about 10 summer students. Two career development post-Doctoral fellows have been associated with the group. Dr. Andrew Lindsay being funded by a HRB-Marie Curie post-Doctoral mobility fellowship to work on secondment for a period in the Curie Institute, Paris, on the functional characterisation of the actin motor proteins (Myosin Vs), and Dr. Conor Horgan funded by the Irish Cancer Society as a research fellow to work on the identification and characterisation of the guanine nucleotide exchange factors (GEFs) which activate Rab25 (Rab11c) during invasive ovarian cancer cell migration.

We maintain a very active collaboration with Dr. Amir Khan’s crystallography group in Trinity College, Dublin on resolving the crystal structures of Rab proteins in complex with their modulatory proteins, particularly with their downstream effector proteins. For an indicative publication in this area, see Lall, P., et.al., 2013. For our list of collaborators past and present see below.

We also have an interest in motor proteins including the dyneins and myosins. Additionally we have a research interest in Hereditary Haemochromatosis (HH), a condition most prevalent in Celtic populations that leads to the gradual build-up of iron in the body due to an iron metabolism disorder, which if undiagnosed can lead to a gradual accumulation of iron in the body. This ultimately can cause destruction of vital organs and lead to death. Several genes / proteins are implicated in HH in particular the HFE gene/protein. My group has investigated the localisation and function of proteins (such as HFE) in the context of iron uptake into cells. For further information regarding our work on HH/HFE see Bhatt, L., et.al., 2007 & 2009.

More recently we have become interested in the GRAB protein and its function in the context of Rab11 cellular function.

For publications from 1995 to present please click here.

For selected publications from 2008 - 2014 please click here.

Research Team:

Current lab members: Career Development

Dr. Andrew Lindsay

PhD Students:

  • Jennifer Timoney
  • Patrycja Artemiuk
  • Dylan O'Donovan (starting June 2015)

Laboratory Manager:

Sara Hanscom


Past Lab Members

Post-Docs/Research Assistants:

  • Dr. Conor Horgan
  • Dr. Eoin Kelly
  • Dr. Arkadiusz Oleksy
  • Mr. Tomek Patzer
  • Dr. Lavinia Bhatt
  • Ms. Siobhan Ryan
  • Dr. Alexander Zhdanov
  • Dr. Nicolas Marie
  • Dr. Tomas Zurawski
  • Dr. Melanie Walsh
  • Dr. Michelle Ryan
  • Dr. Debra Wallace
  • Dr. Silvia Mora
  • Dr. Per Ove Thornqvist

PhD Students:

  • Mr. Andrew Lindsay
  • Mr. Eoin Kelly
  • Mr. Conor Horgan
  • Ms. Niamh Irwin
  • Ms. Aoife Corcoran
  • Ms. Kathleen Fitzgerald
  • Ms. Deborah Wallace
  • Ms. Anna Bielli
  • Mr. Robert Finn
  • Ms. Frances Drummond
  • Mr. Alan Hendrick

MSc Students

  • Mr. Raghu Vamshi Peddapatla
  • Ms. Lydia Riordan
  • Ms. Sylvia O’Sullivan
  • Ms. Deirdre Ni Shuilleabhain
  • Mr. Jason Wade
  • Ms. Frances Drummond

Summer Students

  • Mr. Dylan O'Donovan
  • Ms. Edwina Stack
  • Mr. Martin O'Neill

 

Research in the laboratory has been supported over the years by funding (~7 million Euro) from:

Science Foundation Ireland (SFI)

The European Union (EU)

Irish Cancer Society (ICS)

The Health Research Board of Ireland (HRB)

Research Grants

 ProjectFunding
Body
Start DateEnd DateAward
Functional analysis of the Rzab11 and Rab14 GTPases and their associated proteins in eukaryotic membrane trafficking, with potentila identification of targets for therapeutic intervention in the treatment of human disease.Science Foundation of Ireland01-OCT-1030-SEP-15€1,099,428.00
budget for consumables from R12305Health Research Board01-OCT-1230-SEP-15€51,000.00
Identification and characterisation of guanine nucleotide exchange factors (GEFs) which activate Rab25 during invasive ovarian cancer cell migration.Irish Funded Research01-NOV-1131-OCT-14€204,769.00
RabGTPases and their interacting proteins in health and disease.Science Foundation of Ireland01-SEP-1131-OCT-12€4,000.00
EI CS/2010/0282Enterprise Irl01-AUG-1031-OCT-10€12,000.00
Laser Confocal Microscope RepairScience Foundation of Ireland01-JAN-1030-SEP-10€8,000.00
SFI"Functional Characterisation of Rab11-FIP4 Protein"Science Foundation of Ireland30-AUG-0831-AUG-11€261,578.00
SFI- Summer Student Ashleigh ByrneScience Foundation of Ireland04-JUN-0727-AUG-07€5,600.00
SFI "Functional characteristics of Rab11 & the Class I Rab11-FIP's in endosomal trafficking.."Science Foundation of Ireland01-APR-0630-SEP-10€983,903.00
IRCSET "Functional Characterisation of the RAB11-FIP4 protein"Irish Research Council for Science, Engineering & Technology (IRCSET)01-OCT-0630-SEP-08€96,300.00
SFI Equipment for Underlying GrantScience Foundation of Ireland01-DEC-0630-MAY-07€111,609.00
HRB "Characterisation of the Role of the Human Myosin V Family in Cell Division and Disease"Health Research Board01-OCT-0501-OCT-08€225,694.00
HRB "A functional molecular and cellular analysis of Haemochromatosis"Health Research Board01-OCT-0531-DEC-08€235,574.00
Dual Polarisation Interferometry Studies on Rab11 effector proteinsFoundation Funded Research01-NOV-0531-OCT-09€80,000.00
Summer Student - Edwina StackScience Foundation of Ireland06-JUN-0529-AUG-05€5,600.00
Characterisation of the localisation and function of the endogenous human ..Science Foundation of Ireland17-JUN-0408-SEP-04€6,800.00
[HPMD-CT-2000-00024] {Dr M McCaffrey } NEuropean Union08-AUG-0207-MAR-06€215,689.00
{McCaffrey,Dr.M} NScience Foundation of Ireland11-NOV-0210-NOV-05€1,008,101.00
[HRB RP 16/2001] {McCaffrey Dr. M.} NHealth Research Board01-OCT-0130-SEP-03€114,276.00
[EU BIO4-CT98-6055] {McCaffrey, Dr.M} NEuropean Union01-JUL-9831-DEC-01€225,036.00
[ERBFMRXCT960020 TMR] {Dr.M.MCCAFFREY} NEuropean Union01-AUG-9631-JUL-00€190,461.00

Publications

Peer Reviewed Journals

 YearPublication
(2014)'Myosin Va is Required for the Transport of Fragile X Mental Retardation Protein (FMRP) Granules'
Lindsay AJ, McCaffrey MW (2014) 'Myosin Va is Required for the Transport of Fragile X Mental Retardation Protein (FMRP) Granules'. Biology of The Cell / Under The Auspices of The European Cell Biology Organization,   [DOI] [Details]
(2013)'GRAB is a binding partner for the Rab11a and Rab11b GTPases'
Horgan CP, Hanscom SR, McCaffrey MW (2013) 'GRAB is a binding partner for the Rab11a and Rab11b GTPases'. Biochemical Biophysical Research Communications, 441 (1):214-219   [DOI] [Details]
(2013)'Structural and functional analysis of FIP2 binding to the endosome-localised Rab25 GTPase'
Lall, P,Horgan, CP,Oda, S,Franklin, E,Sultana, A,Hanscom, SR,McCaffrey, MW,Khan, AR (2013) 'Structural and functional analysis of FIP2 binding to the endosome-localised Rab25 GTPase'. Biochimica Et Biophysica Acta-Proteins And Proteomics, 1834 :2679-2690 [DOI] [Details]
(2013)'Identification and characterization of multiple novel Rab-myosin Va interactions'
Lindsay AJ, Jollivet F, Horgan CP, Khan AR, Raposo G, McCaffrey MW, Goud B (2013) 'Identification and characterization of multiple novel Rab-myosin Va interactions'. Molecular biology of the cell, 24 (21):3420-3434 [DOI] [Details]
(2013)'A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes'
Reed SE, Hodgson LR, Song S, May MT, Kelly EE, McCaffrey MW, Mastick CC, Verkade P, Tavaré JM (2013) 'A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes'. Journal of Cell Science, 126 (9):1931-1941   [DOI] [Details]
(2012)'Roles for myosin Va in RNA transport and turnover'
McCaffrey MW, Lindsay AJ (2012) 'Roles for myosin Va in RNA transport and turnover'. Biochemical Society transactions, 40 (6):1416-1420 [DOI] [Details]
(2012)'Roles for myosin Va in RNA transport and turnover'
McCaffrey, MW,Lindsay, AJ (2012) 'Roles for myosin Va in RNA transport and turnover'. Biochemical Society Transactions, 40 :1416-1420 [DOI] [Details]
(2012)'Role of the Rab11 pathway in negative-strand virus assembly'
Bruce, EA,Stuart, A,McCaffrey, MW,Digard, P (2012) 'Role of the Rab11 pathway in negative-strand virus assembly'. Biochemical Society Transactions, 40 :1409-1415 [DOI] [Details]
(2012)'Rab11 proteins in health and disease'
Kelly EE, Horgan CP, McCaffrey MW (2012) 'Rab11 proteins in health and disease'. Biochemical Society Transactions, 40 (6):1360-1367 [DOI] [Details]
(2012)'The Rab family of proteins: 25 years on'
Kelly EE, Horgan CP, Goud B, McCaffrey MW (2012) 'The Rab family of proteins: 25 years on'. Biochemical Society Transactions, 40 (6):1337-1347 [DOI] [Details]
(2012)'Tumor Susceptibility Gene 101 (TSG101) Is a Novel Binding-Partner for the Class II Rab11-FIPs'
Horgan, CP,Hanscom, SR,Kelly, EE,McCaffrey, MW (2012) 'Tumor Susceptibility Gene 101 (TSG101) Is a Novel Binding-Partner for the Class II Rab11-FIPs'. Plos One, 7 [DOI] [Details]
(2012)'Diacylglycerol kinase alpha controls RCP-dependent integrin trafficking to promote invasive migration'
Rainero, E,Caswell, PT,Muller, PAJ,Grindlay, J,McCaffrey, MW,Zhang, QF,Wakelam, MJO,Vousden, KH,Graziani, A,Norman, JC (2012) 'Diacylglycerol kinase alpha controls RCP-dependent integrin trafficking to promote invasive migration'. The Journal of Cell Biology, 196 :277-295 [DOI] [Details]
(2012)'Endosomal trafficking in animal cytokinesis'
Horgan CP, McCaffrey MW (2012) 'Endosomal trafficking in animal cytokinesis'. Frontiers in bioscience (Scholar edition), 4 :547-555 [Details]
(2012)'Rab30 is required for the morphological integrity of the Golgi apparatus'
Kelly, EE,Giordano, F,Horgan, CP,Jollivet, F,Raposo, G,McCaffrey, MW (2012) 'Rab30 is required for the morphological integrity of the Golgi apparatus'. Biology of The Cell / Under The Auspices of The European Cell Biology Organization, 104 :84-101 [DOI] [Details]
(2011)'Rab GTPases and microtubule motors'
Horgan, CP,McCaffrey, MW (2011) 'Rab GTPases and microtubule motors'. Biochemical Society Transactions, 39 :1202-1206 [DOI] [Details]
(2011)'Myosin Va Is Required for P Body but Not Stress Granule Formation'
Lindsay, AJ,McCaffrey, MW; (2011) 'Myosin Va Is Required for P Body but Not Stress Granule Formation'. The Journal of Biological Chemistry, 286 :11519-11528 [DOI] [Details]
(2011)'Dynein LIC1 localizes to the mitotic spindle and midbody and LIC2 localizes to spindle poles during cell division'
Horgan CP, Hanscom SR, McCaffrey MW; (2011) 'Dynein LIC1 localizes to the mitotic spindle and midbody and LIC2 localizes to spindle poles during cell division'. Cell biology international, 35 (2):171-178 [DOI] [Details]
(2011)'The role of endosomal-recycling in long-term potentiation'
Kelly EE, Horgan CP, McCaffrey MW, Young P; (2011) 'The role of endosomal-recycling in long-term potentiation'. Cellular and Molecular Life Sciences, 68 (2):185-194 [DOI] [Details]
(2010)'N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding'
Bhatt, L,Murphy, C,O'Driscoll, LS,Carmo-Fonseca, M,McCaffrey, MW,Fleming, JV; (2010) 'N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding'. Febs Journal, 277 :3219-3234 [DOI] [Details]
(2010)'Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis'
Schwertassek U, Buckley DA, Xu CF, Lindsay AJ, McCaffrey MW, Neubert TA, Tonks NK; (2010) 'Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis'. Febs Journal, 277 (11):2463-2473 [DOI] [Details]
(2010)'Rab11-FIP3 binds dynein light intermediate chain 2 and its overexpression fragments the Golgi complex'
Horgan, CP; Hanscom, SR; Jolly, RS; Futter, CE; McCaffrey, MW; (2010) 'Rab11-FIP3 binds dynein light intermediate chain 2 and its overexpression fragments the Golgi complex'. Biochemical and Biophysical Research Communications, [DOI] [Details]
(2010)'Rab11-FIP3 links the Rab11 GTPase and cytoplasmic dynein to mediate transport to the endosomal-recycling compartment'
Horgan, CP; Hanscom, SR; Jolly, RS; Futter, CE; McCaffrey, MW; (2010) 'Rab11-FIP3 links the Rab11 GTPase and cytoplasmic dynein to mediate transport to the endosomal-recycling compartment'. Journal of Cell Science, 123 (Pt 2):181-191 [DOI] [Details]
(2009)'The dynamic Rab11-FIPs'
Horgan, CP; McCaffrey, MW; (2009) 'The dynamic Rab11-FIPs'. Biochemical Society Transactions, 37 (Pt 5):1032-1036 [DOI] [Details]
(2009)'Class I Rab11-Family Interacting Proteins are binding targets for the Rab14 GTPase'
Kelly EE, Horgan CP, Adams C, Patzer TM, Ní Shúilleabháin DM, Norman JC, McCaffrey MW; (2009) 'Class I Rab11-Family Interacting Proteins are binding targets for the Rab14 GTPase'. Biology of The Cell / Under The Auspices of The European Cell Biology Organization, [DOI] [Details]
(2009)'Myosin Vb localises to nucleoli and associates with the RNA polymerase I transcription complex'
Lindsay AJ, McCaffrey MW; (2009) 'Myosin Vb localises to nucleoli and associates with the RNA polymerase I transcription complex'. Cell Motility and The Cytoskeleton, [DOI] [Details]
(2009)'Knockdown of beta 2-microglobulin perturbs the subcellular distribution of HFE and hepcidin'
Bhatt, L,Horgan, CP,McCaffrey, MW; (2009) 'Knockdown of beta 2-microglobulin perturbs the subcellular distribution of HFE and hepcidin'. Biochemical and Biophysical Research Communications, 378 :727-731 [DOI] [Details]
(2008)'Rab-coupling protein coordinates recycling of alpha5beta1 integrin and EGFR1 to promote cell migration in 3D microenvironments'
Caswell PT, Chan M, Lindsay AJ, McCaffrey MW, Boettiger D, Norman JC; (2008) 'Rab-coupling protein coordinates recycling of alpha5beta1 integrin and EGFR1 to promote cell migration in 3D microenvironments'. The Journal of Cell Biology, 183 (1):143-155 [DOI] [Details]
(2007)'Rip11 is a Rab11-and AS160-RabGAP-binding protein required for insulin-stimulated glucose uptake in adipocytes'
Welsh, GI,Leney, SE,Lloyd-Lewis, B,Wherlock, M,Lindsay, AJ,McCaffrey, MW,Tavare, JM (2007) 'Rip11 is a Rab11-and AS160-RabGAP-binding protein required for insulin-stimulated glucose uptake in adipocytes'. Journal of Cell Science, 120 :4197-4208 [DOI] [Details]
(2007)'Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments'
Caswell PT, Spence HJ, Parsons M, White DP, Clark K, Cheng KW, Mills GB, Humphries MJ, Messent AJ, Anderson KI, McCaffrey MW, Ozanne BW, Norman JC; (2007) 'Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments'. Developmental Cell, 13 (4):496-510 [DOI] [Details]
(2007)'The Hereditary Hemochromatosis protein HFE and its chaperone beta 2-microglobulin localise predominantly to the endosomal-recycling compartment'
Bhatt, L,Horgan, CP,Walsh, M,McCaffrey, MW; (2007) 'The Hereditary Hemochromatosis protein HFE and its chaperone beta 2-microglobulin localise predominantly to the endosomal-recycling compartment'. Biochemical and Biophysical Research Communications, 359 :277-284 [DOI] [Details]
(2007)'Rab11-FIP3 is critical for the structural integrity of the endosomal recycling compartment'
Horgan CP, Oleksy A, Zhdanov AV, Lall PY, White IJ, Khan AR, Futter CE, McCaffrey JG, McCaffrey MW; (2007) 'Rab11-FIP3 is critical for the structural integrity of the endosomal recycling compartment'. Traffic (Copenhagen, Denmark), 8 (4):414-430 [DOI] [Details]
(2006)'Crystal structure of Rab11 in complex with Rab11 family interacting protein 2'
Jagoe, WN,Lindsay, AJ,Read, RJ,Mccoy, AJ,McCaffrey, MW,Khan, AR; (2006) 'Crystal structure of Rab11 in complex with Rab11 family interacting protein 2'. Structure, 14 :1273-1283 [DOI] [Details]
(2006)'Purification, crystallization and preliminary X-ray diffraction studies of Rab11 in complex with Rab11-FIP2'
Jagoe WN, Jackson SR, Lindsay AJ, McCaffrey MW, Khan AR; (2006) 'Purification, crystallization and preliminary X-ray diffraction studies of Rab11 in complex with Rab11-FIP2'. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 62 (Pt 7):692-694 [DOI] [Details]
(2005)'Rab coupling protein is selectively degraded by calpain in a Ca2+-dependent manner'
Marie, N,Lindsay, AJ,McCaffrey, MW; (2005) 'Rab coupling protein is selectively degraded by calpain in a Ca2+-dependent manner'. The Biochemical Journal, 389 :223-231 [DOI] [Details]
(2004)'Rab coupling protein associates with phagosomes and regulates recycling from the phagosomal compartment'
Damiani, MT,Pavarotti, M,Leiva, N,Lindsay, AJ,McCaffrey, MW,Colombo, MI; (2004) 'Rab coupling protein associates with phagosomes and regulates recycling from the phagosomal compartment'. Traffic (Copenhagen, Denmark), 5 :785-797 [DOI] [Details]
(2004)'The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane'
Lindsay AJ, McCaffrey MW; (2004) 'The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane'. Journal of Cell Science, 117 (Pt 19):4365-4375 [DOI] [Details]
(2004)'Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis'
Lindsay AJ, McCaffrey MW; (2004) 'Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis'. Febs Letters, 571 (1-3):86-92 [DOI] [Details]
(2004)'Rab11-FIP3 localises to a Rab11 positive preicentrosomal compartment during interphrase and to the cleavage furrow during cytokinesis'
Horgan, CP; Walsh, M; Zurawski, TH; McCaffrey, MW; (2004) 'Rab11-FIP3 localises to a Rab11 positive preicentrosomal compartment during interphrase and to the cleavage furrow during cytokinesis'. Biochem Biophys Res Commun, 319 :83-94 [Details]
(2004)'Distinct localization and functional properties of two mammalian class V myosins during cell cycle progression'
Lindsay, AJ,McCaffrey, MW (2004) 'Distinct localization and functional properties of two mammalian class V myosins during cell cycle progression'. Biology of The Cell / Under The Auspices of The European Cell Biology Organization, 15 :29-29 [Details]
(2003)'The mechanism of internalization of glycosylphosphatidylinositol-anchored prion protein'
Sunyach, C,Jen, A,Deng, J,Fitzgerald, KT,Frobert, Y,Grassi, J,McCaffrey, MW,Morris, R; (2003) 'The mechanism of internalization of glycosylphosphatidylinositol-anchored prion protein'. The Embo Journal, 22 :3591-3601 [Details]
(2002)'Rab11-FIP4 interacts with Rab11 in a GTP-dependent manner and its overexpression condenses the Rab11 positive compartment in HeLa cells'
Wallace, DME,Lindsay, AJ,Hendrick, AG,McCaffrey, MW; (2002) 'Rab11-FIP4 interacts with Rab11 in a GTP-dependent manner and its overexpression condenses the Rab11 positive compartment in HeLa cells'. Biochemical and Biophysical Research Communications, 299 :770-779 [DOI] [Details]
(2002)'Rab11-FIP2 functions in transferrin recycling and associates with endosomal membranes via its COOH-terminal domain'
Lindsay, AJ,McCaffrey, MW; (2002) 'Rab11-FIP2 functions in transferrin recycling and associates with endosomal membranes via its COOH-terminal domain'. The Journal of Biological Chemistry, 277 :27193-27199 [DOI] [Details]
(2002)'The novel Rab11-FIP/Rip/RCP family of proteins displays extensive homo- and hetero-interacting abilities'
Wallace, DM,Lindsay, AJ,Hendrick, AG,McCaffrey, MW; (2002) 'The novel Rab11-FIP/Rip/RCP family of proteins displays extensive homo- and hetero-interacting abilities'. Biochemical and Biophysical Research Communications, 292 :909-915 [DOI] [Details]
(2002)'Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein'
Lindsay, AJ,Hendrick, AG,Cantalupo, G,Senic-Matuglia, F,Goud, B,Bucci, C,McCaffrey, MW; (2002) 'Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein'. The Journal of Biological Chemistry, 277 :12190-12199 [DOI] [Details]
(2001)'Rab4 affects both recycling and degradative endosomal trafficking'
McCaffrey, MW; Bielli, A; Cantalupa, G; Mora, S; Roberti, V; Santilla, RM; Drummond, F; Bucci, C.; (2001) 'Rab4 affects both recycling and degradative endosomal trafficking'. FEBS Letters, 495 :21-30 [Details]
(2001)'The small GTPase Rab4A interacts with the central region of cytoplasmic dynein light intermediate chain-1'
Bielli, A; Thornqvist, PO; Hendrick, AG; Finn, R; Fitzgeraldm K; McCaffrey, MW.; (2001) 'The small GTPase Rab4A interacts with the central region of cytoplasmic dynein light intermediate chain-1'. Biochem Biophys Res Commun, 281 (*):1141-1153 [Details]
(1998)'Distinct Rab binding domains mediate the interaction of Raaptin-5 with GTP-bound rab4 and rab5'
Vitale, G; Rybin, V; Christoforidis, S; Thronqvist, PO; McCaffrey, MW; Stenmark, H; Zerial, M.; (1998) 'Distinct Rab binding domains mediate the interaction of Raaptin-5 with GTP-bound rab4 and rab5'. The EMBO Journal, 17 :1941-1951 [Details]
(1995)'Construction of a recombinant HRab1A baculovirus'
Drummond, FJ, McCaffrey MW (1995) 'Construction of a recombinant HRab1A baculovirus'. Biochemical Society Transactions, 23 :380-380 [Details]
(1995)'Studies on the GTP binding ability of the human Rab4p, expressed in E. coli'
O'Brien F, McCaffrey MW (1995) 'Studies on the GTP binding ability of the human Rab4p, expressed in E. coli'. Biochemical Society Transactions, 23 :377-377 [Details]
(1993)'Comparison of the biochemical properties of unprocessed and processed forms of Rab6p'
Yang C, Mollat P, McCaffrey M (1993) 'Comparison of the biochemical properties of unprocessed and processed forms of Rab6p'. European Journal of Biochemistry, 217 :1027-1037 [Details]
(1991)'The small GTP-binding protein Rho1p is localized on the Golgi apparatus and post-Golgi vesicles in S. cerevisiae'
McCaffrey M, Johnson J, Goud B, Myers A, Rossier J, Popoff M, Madaule P, Boquet P (1991) 'The small GTP-binding protein Rho1p is localized on the Golgi apparatus and post-Golgi vesicles in S. cerevisiae'. Journal of Cell Biology, 115 :309-319 [Details]
(1991)'Small GTP-binding proteins and their role in transport'
Goud, B; McCaffrey, MW; (1991) 'Small GTP-binding proteins and their role in transport'. Current Opinion Cell Biology, 4 :626-633 [Details]
(1991)'Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2'
Bailly E, McCaffrey M, Touchot N, Zahraoui A, Goud B, Bornens M; (1991) 'Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2'. Nature, 350 (6320):715-718 [DOI] [Details]

Book Chapters

 YearPublication
(2005)'Purification and functional properties of Rab11-FIP3'
Horgan CP, Zurawski TH, McCaffrey MW; (2005) 'Purification and functional properties of Rab11-FIP3' In: Methods in enzymology. [DOI] [Details]
(2005)'Pruification and Functional properties of Rab11-FIP2'
Lindsay, A.J., McCaffrey, M.W.; (2005) 'Pruification and Functional properties of Rab11-FIP2' In: Methods in Enzymology. USA: Academic Press. [Details]
(2004)'The Rab family'
McCaffrey, M.W. and Lindsay, A.J; (2004) 'The Rab family' In: Encyclopedia of Biological Chemistry, Vol. 3. London: Academic Press/Elsevier Science. [Details]
(1998)'Intracelluar membrane trafic in From Cell to Brain: Intra- and Inter-Cellular Communication'
McCaffrey, M; Goud, B; (1998) 'Intracelluar membrane trafic in From Cell to Brain: Intra- and Inter-Cellular Communication' In: The Central Nervous System. UK: Elsevier Science. [Details]
(1995)'Rho1p function in Saccharomyces cerevisiae'
Madaule, P., Johnson J., McCaffrey, M., Boquet, P, Myers A.; (1995) 'Rho1p function in Saccharomyces cerevisiae' In: The Handbook of small GTPases. UK: Oxford University Press. [Details]
(2005)'Functional Properties of the Rab-Binding Domain of Rab Coupling Protein'
Lindsay AJ, Marie N, McCaffrey MW (2005) 'Functional Properties of the Rab-Binding Domain of Rab Coupling Protein' In: W. Balch, C. Der, A. Hall (eds). Methods in Enzymology. NA: Academic Press. [Details]

Professional Activities

Professional Associations

 AssociationFunctionFrom / To
Biochemical Society Council Member01-JAN-15 /
Biochemical Society - Cell Biology Panel Chair11-AUG-11 / 31-DEC-14
Biochemical Society - Cell Biology Panel Vice Chair01-JAN-08 / 31-DEC-10
Biochemical Society - Cell Biology Panel Member01-JAN-04 / 31-DEC-07
Biochemical Society Member01-JAN-92 / 31-DEC-25

Conference Contributions

 YearPublication
(2009)'Dynamic Cell' meeting of Biochemical Society/British Society for Cell Biology,
McCaffrey, M.W; (2009) Rabs in dynamic cellular processes session. [Conference Organising Committee Chairperson], 'Dynamic Cell' meeting of Biochemical Society/British Society for Cell Biology, Edinburgh, UK , 01-APR-09 - 04-APR-09. [Details]
(2009)3rd UCC Biochemistry Department 'Talking Science' Research Day,
Horgan CP*, Hanscom SR and McCaffrey MW (2009) FIP3 Mediates Transport of Material to the Endosomal-Recycling Compartment by Linking the Rab11 GTPase to Cytoplasmic Dynein. [Poster Presentation], 3rd UCC Biochemistry Department 'Talking Science' Research Day, Cork, Ireland , 09-APR-09 - 09-APR-09. [Details]
(2009)Joint Biochemical Society/British Society for Cell Biology Meeting - ‘The Dynamic Cell’,
Horgan CP*, Hanscom SR and McCaffrey MW (2009) FIP3 Mediates Transport of Material to the Endosomal-Recycling Compartment by Linking the Rab11 GTPase to Cytoplasmic Dynein. [Poster Presentation], Joint Biochemical Society/British Society for Cell Biology Meeting - ‘The Dynamic Cell’, Edinburgh, Scotland , 01-APR-09 - 04-APR-09. [Details]
(2008)Science Foundation Ireland (SFI) Science Summit 2008,
Horgan CP, Hanscom SR and McCaffrey MW* (2008) FIP3 Mediates Transport of Material to the Endosomal-Recycling Compartment by Linking the Rab11 GTPase to Cytoplasmic Dynein. [Poster Presentation], Science Foundation Ireland (SFI) Science Summit 2008, Kilkenny, Ireland , 17-NOV-08 - 18-NOV-08. [Details]
(2008)Towards an understanding of Rab11 controlled cellular function,
McCaffrey, M.W; (2008) Towards an understanding of Rab11 controlled cellular function. [Oral Presentation], Towards an understanding of Rab11 controlled cellular function, Curie Institute, Paris , 03-OCT-08 - 03-OCT-08. [Details]
(2008)Rab11, the Rab11-FIPs and motor proteins,
McCaffrey, M.W; (2008) Rab11, the Rab11-FIPs and motor proteins. [Oral Presentation], Rab11, the Rab11-FIPs and motor proteins, Beatson Institute Glasgow , 23-SEP-08 - 23-SEP-08. [Details]
(2008)Beatson Institute Workshop – Vesicular Transport and Cancer,
Horgan CP*, Hanscom SR and McCaffrey MW (2008) FIP3 Mediates Transport of Material to the Endosomal-Recycling Compartment by Linking the Rab11 GTPase to Cytoplasmic Dynein. [Poster Presentation], Beatson Institute Workshop – Vesicular Transport and Cancer, Glasgow, Scotland , 21-SEP-08 - 23-SEP-08. [Details]
(2008)2nd UCC Biochemistry Department 'Talking Science' Research Day,
Horgan CP*, Oleksy A, Zhdanov AV, Lall PY, White IJ, Khan AR, Futter CE, McCaffrey JG and McCaffrey MW (2008) Rab11-FIP3 is an Indispensable Component of the Endosomal-Recycling Compartment Machinery. [Poster Presentation], 2nd UCC Biochemistry Department 'Talking Science' Research Day, Cork, Ireland , 15-AUG-08 - 15-AUG-08. [Details]
(2008)Inaugural UCC Biochemistry Department 'Talking Science' Research Day,
Horgan CP* and McCaffrey MW (2008) Rab11-FIP3 – A Lot Done, More to Do. [Invited Oral Presentation], Inaugural UCC Biochemistry Department 'Talking Science' Research Day, Cork, Ireland , 08-FEB-08 - 08-FEB-08. [Details]
(2008)Inaugural UCC Biochemistry Department 'Talking Science' Research Day,
Oleksy A*, Horgan CP, Zhdanov AV, Lall PY, White IJ, Khan AR, Futter CE, McCaffrey JG and McCaffrey MW (2008) Rab11-FIP3 serves a critical role in maintaining the structural integrity of the endosomal-recycling compartment (ERC). [Poster Presentation], Inaugural UCC Biochemistry Department 'Talking Science' Research Day, Cork, Ireland , 08-FEB-08 - 08-FEB-08. [Details]
(2007)Science Foundation Ireland (SFI) Science Summit 2007,
Horgan CP*, Oleksy A, Zhdanov AV, Lall PY, White IJ, Khan AR, Futter CE, McCaffrey JG and McCaffrey MW (2007) A Rab11-Mediated Role for Rab11-FIP3 in Maintaining the Structural Integrity of the Endosomal-Recycling Compartment. [Poster Presentation], Science Foundation Ireland (SFI) Science Summit 2007, Dublin, Ireland , 26-NOV-07 - 27-NOV-07. [Details]
(2007)EMBO workshop: 'Endocytic systems: mechanism and function',
Oleksy A*, Horgan CP, Zhdanov AV, Lall PY, White IJ, Khan AR, Futter CE, McCaffrey JG and McCaffrey MW (2007) Rab11-FIP3 serves a critical role in maintaining the structural integrity of the endosomal-recycling compartment (ERC). [Poster Presentation], EMBO workshop: 'Endocytic systems: mechanism and function', Geneva, Switzerland , 18-SEP-07 - 23-SEP-07. [Details]
(2007)Joint American Society for Cell Biology (ASCB)/European Cytoskeleton Forum (ECF) Summer Meeting. “Dynamic Interplay between Cytoskeletal and Membrane Systems,
Horgan CP*, Oleksy A, Zhdanov AV, Lall PY, White IJ, Khan AR, Futter CE, McCaffrey JG and McCaffrey MW (2007) A Rab11-Mediated Role for Rab11-FIP3 in Maintaining the Structural Integrity of the Endosomal-Recycling Compartment. [Poster Presentation], Joint American Society for Cell Biology (ASCB)/European Cytoskeleton Forum (ECF) Summer Meeting. “Dynamic Interplay between Cytoskeletal and Membrane Systems, Dijon, France , 27-JUN-07 - 30-JUN-07. [Details]
(2007)Irish Association for Cancer Research (IACR) Annual Meeting,
Horgan CP* and McCaffrey MW (2007) Rab11-FIP3 couples Rab11 and ARF6 to deliver endosomally-derived material to the cleavage furrow/midbody during cytokinesis. [Poster Presentation], Irish Association for Cancer Research (IACR) Annual Meeting, Cork, Ireland , 02-MAR-07 - 03-MAR-07. [Details]
(2006)The American Society for Cell Biology (ASCB) Annual Meeting,
Horgan CP, Lindsay AJ*, Zhdanov AV, Oleksy A, Lall PY, McCaffrey JG and McCaffrey MW (2006) Rab11-FIP3 is required to maintain the endosomal-recycling compartment. [Poster Presentation], The American Society for Cell Biology (ASCB) Annual Meeting, San Diego, California, USA , 09-DEC-06 - 13-DEC-06. [Details]
(2006)Royal Microscopical Society – British Society for Cell Biology (RMS-BSCB), Imaging Membrane Dynamics Meeting,
Horgan CP*, Zhdanov AV, Oleksy A, Lall PY, McCaffrey JG and McCaffrey MW (2006) Rab11-FIP3 is critical for the structural integrity of the endosomal-recycling compartment. [Oral Presentation], Royal Microscopical Society – British Society for Cell Biology (RMS-BSCB), Imaging Membrane Dynamics Meeting, Egham, England , 14-SEP-06 - 17-SEP-06. [Details]
(2005)Irish Research Council for Science, Engineering and Technology (IRCSET) National Research Symposium,
Horgan CP* and McCaffrey MW (2005) Characterisation of the Rab11-Family Interacting Protein 3 (Rab11-FIP3) in Eukaryotic membrane trafficking. [Poster Presentation], Irish Research Council for Science, Engineering and Technology (IRCSET) National Research Symposium, Dublin, Ireland , 03-NOV-05 - 03-NOV-05. [Details]
(2005)Irish Area Section (IAS) Biochemical Society Pre-doctoral Meeting,
Horgan CP* and McCaffrey MW (2005) Characterisation of the Rab11-FIP3 protein in endosomal-recycling and cytokinesis. [Invited Oral Presentation], Irish Area Section (IAS) Biochemical Society Pre-doctoral Meeting, Maynooth, Ireland , 07-OCT-05 - 07-OCT-05. [Details]
(2004)Irish Research Council for Science, Engineering and Technology (IRCSET) National Research Symposium,
Horgan CP*, Walsh M, Zurawski TH and McCaffrey MW (2004) Rab11-FIP3, an endosomal-recycling compartment associated protein, relocates to the cleavage furrow during cytokinesis. [Poster Presentation], Irish Research Council for Science, Engineering and Technology (IRCSET) National Research Symposium, Dublin, Ireland , 02-NOV-04 - 02-NOV-04. [Details]
(2004)Joint EMBO/FEBS Workshop, organised by the EURESCO Office - European Science Foundation (ESF) 'Frontiers of Cellular Microbiology and Cell Biology; Spatial and Temporal Dynamics of the Endomembrane System',
Horgan CP*, Walsh M, Zurawski TH and McCaffrey MW (2004) Rab11-FIP3, an endosomal-recycling compartment associated protein, relocates to the cleavage furrow during cytokinesis. [Poster Presentation], Joint EMBO/FEBS Workshop, organised by the EURESCO Office - European Science Foundation (ESF) 'Frontiers of Cellular Microbiology and Cell Biology; Spatial and Temporal Dynamics of the Endomembrane System', San Feliu de Guixols, Spain , 16-OCT-04 - 21-OCT-04. [Details]
(2004)Biosciences Institute (BSI) Seminar Series 2004,
Horgan CP* and McCaffrey MW (2004) Towards an understanding of Rab11-FIP3 function…. [Invited Oral Presentation], Biosciences Institute (BSI) Seminar Series 2004, Cork, Ireland , 05-OCT-04 - 05-OCT-04. [Details]
(2004)The American Society for Cell Biology (ASCB) Summer Meeting on Cytokinesis,
Horgan CP, Walsh M, Zurawski TH and McCaffrey MW* (2004) Rab11-FIP3 localises to a Rab11-positive pericentrosomal compartment during interphase and to the cleavage furrow during cytokinesis. [Poster Presentation], The American Society for Cell Biology (ASCB) Summer Meeting on Cytokinesis, University of Vermont, Burlington, USA , 22-JUL-04 - 25-JUL-04. [Details]
(2003)Irish Research Council for Science, Engineering and Technology (IRCSET) National Research Symposium,
Horgan CP* and McCaffrey MW (2003) Characterisation of the Rab11-FIP3 protein in eukaryotic membrane trafficking. [Poster Presentation], Irish Research Council for Science, Engineering and Technology (IRCSET) National Research Symposium, Dublin, Ireland , 07-NOV-03 - 07-NOV-03. [Details]

Committees

 CommitteeFunctionFrom / To
Biochemical Society (UK & Irl) Chair of the Cell Biology Panel2011 / 2014

Employment

 EmployerPositionFrom / To
University College Cork Associate Professor in Biochemistry01-OCT-09 /
Curie Institute, Paris Visiting Scientist01-JAN-98 / 31-DEC-98
Pasteur Institute, Paris Post Doctoral Fellow01-JAN-90 / 31-DEC-91
CNRS, Gif-sur-Yvette, France Post Doctoral Fellow01-JAN-88 / 31-DEC-89

Education

 YearInstitutionQualificationSubject
1983National University of Ireland BScMicrobiology
1987University of London PhD

Languages

 LanguageReadingWritingSpeaking
Spanish FunctionalFunctionalFunctional
French FluentFunctionalFluent

Outreach Activities

 Description

I participate actively in the SFI Outreach Programme of scientists visiting schools to speak to students at both primary and post-primary level.

For further information on such outreach talks that I have given please click here.

Journal Activities

 JournalRoleTo / From
Biol Cell Referee-
Biochemical Society Transactions Referee-
Febs Lett Referee-
J Cell Sci Referee-
Traffic Referee-

Other Activities

 Description

Ph.D. Theses External Exammining: United Kingdon: University of Liverpool; University of Sheffield; University of Bristol. France: University of Paris Sud/Institut Curie, Paris. Ireland: University of Dublin, Trinity College.

Grant Referee for the following granting bodies: Wellcome Trust, UK; Medical Research Council, UK; BBSRC, UK; British Heart Foundation, UK

Conference Organisation: 1) Organised the "Rab" Session of the 2009 Dynamic Cell, Edinburgh, UK. 2) Organised the "Rab GTPases in Health and Disease" conference, 2012, UCC, Cork, Ireland.

1998, Sabbatical year working as a visiting student on development of GFP-Rab stable lines and two-hybrid work in Curie Institut, Paris.


From 1991-1997, short (4-6 weeks) training visits were made each summer to different laboratories such as the Pasteur Inst in Paris, EMBL in Heidelberg and the Curie Institute also in Paris to acquire new techniques such as immunofluorescence and confocal microscopy, and two-hybrid methodology.

Teaching Activities

Teaching Interests

I currently teach various aspects of molecular biology, cell signalling and cell biology to undergraduate science & medical students. An outline of my courses and all courses taught by staff in the Department of Biochemistry are available at UCC Book of Modules.

Current Postgraduate Students

 StudentDegree Type
Artemiuk Patrycja Anna Masters Degree by Research
Timoney Jennifer Anne Doctoral Degree (Structured)

Modules Taught

 Term (ID))TitleLinkSubject
2015Cancer Biology/Molecular Oncology BC4009Cancer Biology/Molecular Oncology
2015Cell Signalling BC3004Cell Signalling

Research Information

External Collaborators

 NameOrganisation/ InstitutionCountry
Anton Bennett Yale UniversityU.S.A.
Paul Digard The Roslin InstituteUNITED KINGDOM
Terje Espevik & Harald Husebye The Norwegian University of Science & TechnologyNORWAY
Clare Futter University College LondonUNITED KINGDOM
Bruno Goud Institut CurieFRANCE
Clare Jolly University College LondonUNITED KINGDOM
Amir Khan Trinity CollegeIRELAND
Yuichiro Nakatsu & Makoto Takeda National Institute of Infectious DiseasesJAPAN
Jim Norman Beatson Institute for Cancer ResearchUNITED KINGDOM
Graca Raposo Institut CurieFRANCE
Trina Schroer Johns Hopkins UniversityU.S.A.
Jeremy Tavare University of BristolUNITED KINGDOM

Contact details

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School of Biochemistry and Cell Biology

Scoil na Bithcheimice agus na Cillbhitheolaíochta

University College Cork, Western Road, Cork.

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