Research Profile

John V (Eoin) Fleming

Biography

Total Career Publications: 21

Publication 2008-2014: 3

Total Citations: 595

h-index: 14

Total Career Research Income: €597,000

PhDs graduated (Career): 1

PhDs graduated (2008-2014): 1

Other relevant research information:


Research Interests

Research in my lab is focussed on the expression, function and degradation of ER associated proteins. Our published results relate to two proteins in particular, the histidine decarboxylase (HDC) enzyme that catalyzes the formation of histamine, and HFE, which is involved in regulating cellular iron metabolism. Treatment of histamine related diseases can conservatively be estimated to cost Irish patients many millions of euros every year, while the most common inherited disease in Ireland, hereditary haemachromatosis, is associated with mutations in the HFE gene. We are particularly interested in characterizing the manner in which these two proteins are degraded with the aim of providing new insights into how they are regulated physiologically, or how they could potentially be regulated pharmacologically.

Publications

Peer Reviewed Journals

 YearPublication
(2014)'The human ubiquitin conjugating enzyme UBE2J2 (Ubc6) is a substrate for proteasomal degradation'
Lam, SY,Murphy, C,Foley, LA,Ross, SA,Wang, TC,Fleming, JV (2014) 'The human ubiquitin conjugating enzyme UBE2J2 (Ubc6) is a substrate for proteasomal degradation'. Biochemical and Biophysical Research Communications, 451 :361-366 [DOI] [Details]
(2014)'Differential processing of mammalian L-histidine decarboxylase enzymes'
Fennell, LM,Fleming, JV (2014) 'Differential processing of mammalian L-histidine decarboxylase enzymes'. Biochemical and Biophysical Research Communications, 445 :304-309 [DOI] [Details]
(2010)'N-glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding'
Bhatt L, Murphy C, O'Driscoll LS, Carmo-Fonseca M, McCaffrey MW, Fleming JV; (2010) 'N-glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding'. The FEBS journal, 277 (15):3219-3234 [DOI] [Details]
(2007)'Chemical chaperones reduce endoplasmic reticulum stress and prevent mutant HFE aggregate formation'
de Almeida SF, Picarote G, Fleming JV, Carmo-Fonseca M, Azevedo JE, de Sousa M; (2007) 'Chemical chaperones reduce endoplasmic reticulum stress and prevent mutant HFE aggregate formation'. The Journal of biological chemistry, 282 (38):27905-27912 [DOI] [Details]
(2007)'Stimulation of an unfolded protein response impairs MHC class I expression'
de Almeida SF, Fleming JV, Azevedo JE, Carmo-Fonseca M, de Sousa M; (2007) 'Stimulation of an unfolded protein response impairs MHC class I expression'. Journal of immunology (Baltimore, Md. : 1950), 178 (6):3612-3619 [Details]
(2007)'Gastrin regulates the TFF2 promoter through gastrin-responsive cis-acting elements and multiple signaling pathways'
Tu S, Chi AL, Lim S, Cui G, Dubeykovskaya Z, Ai W, Fleming JV, Takaishi S, Wang TC; (2007) 'Gastrin regulates the TFF2 promoter through gastrin-responsive cis-acting elements and multiple signaling pathways'. American Journal of Physiology - Gastrointestinal and Liver Physiology, 292 (6):1-37 [DOI] [Details]
(2006)'Regulation of L-histidine decarboxylase and its role in carcinogenesis'
Ai W, Takaishi S, Wang TC, Fleming JV; (2006) 'Regulation of L-histidine decarboxylase and its role in carcinogenesis'. Progress In Nucleic Acid Research and Molecular Biology, Vol 81, 81 :231-270 [DOI] [Details]
(2004)'Gastrin-mediated activation of cyclin D1 transcription involves beta-catenin and CREB pathways in gastric cancer cells'
Pradeep A, Sharma C, Sathyanarayana P, Albanese C, Fleming JV, Wang TC, Wolfe MM, Baker KM, Pestell RG, Rana B; (2004) 'Gastrin-mediated activation of cyclin D1 transcription involves beta-catenin and CREB pathways in gastric cancer cells'. Oncogene, 23 (20):3689-3699 [DOI] [Details]
(2004)'Mapping of catalytically important residues in the rat L-histidine decarboxylase enzyme using bioinformatic and site-directed mutagenesis approaches'
Fleming JV, Sánchez-Jiménez F, Moya-García AA, Langlois MR, Wang TC; (2004) 'Mapping of catalytically important residues in the rat L-histidine decarboxylase enzyme using bioinformatic and site-directed mutagenesis approaches'. The Biochemical Journal, 379 (Pt 2):253-261 [DOI] [Details]
(2004)'The C-terminus of rat L-histidine decarboxylase specifically inhibits enzymic activity and disrupts pyridoxal phosphate-dependent interactions with L-histidine substrate analogues'
Fleming JV, Fajardo I, Langlois MR, Sánchez-Jiménez F, Wang TC; (2004) 'The C-terminus of rat L-histidine decarboxylase specifically inhibits enzymic activity and disrupts pyridoxal phosphate-dependent interactions with L-histidine substrate analogues'. The Biochemical Journal, 381 (Pt 3):769-778 [DOI] [Details]
(2003)'The production of 53-55-kDa isoforms is not required for rat L-histidine decarboxylase activity'
Fleming JV, Wang TC; (2003) 'The production of 53-55-kDa isoforms is not required for rat L-histidine decarboxylase activity'. The Journal of Biological Chemistry, 278 (1):686-694 [DOI] [Details]
(2002)'Identification and characterization of a third gastrin response element (GAS-RE3) in the human histidine decarboxylase gene promoter'
Raychowdhury, R., Fleming, J.V., McLoughlin, J.T. Bulitta, C.J. and Wang, T.C.. Biochem. Biophys. Res. Commun; (2002) 'Identification and characterization of a third gastrin response element (GAS-RE3) in the human histidine decarboxylase gene promoter'. Biochemical and Biophysical Research Communications, :1089-1095 [Details]
(2002)'Autoinduction of the trefoil factor 2 (TFF2) promoter requires an upstream cis-acting element'
Bulitta, C.J., Fleming, J.V., Raychowdhury, R., Taupin, D., Rosenberg, I. and Wang, T.C. ; (2002) 'Autoinduction of the trefoil factor 2 (TFF2) promoter requires an upstream cis-acting element'. Biochemical and Biophysical Research Communications, :366-374 [Details]
(2002)'Interaction of Early Growth Response Protein 1 (Egr-1), Sp1 and CREB at a proximal response element is critical for gastrin-dependent activation of the chromogranin A promoter'
Raychowdhury, R., Schafer, G., Fleming, J., Rosewicz, S., Wiedenmann, B., Wang, T.C., and Hocker; (2002) 'Interaction of Early Growth Response Protein 1 (Egr-1), Sp1 and CREB at a proximal response element is critical for gastrin-dependent activation of the chromogranin A promoter'. Journal of Molecular Endocrinology, :2802-2818 [Details]
(2001)'L-histidine decarboxylase decreases its own transcription through down-regulation of ERK activity'
Fleming, J.V., Colucci, R., Xavier, R, Wang, T.C.; (2001) 'L-histidine decarboxylase decreases its own transcription through down-regulation of ERK activity'. American Jnl. Physiology, [Details]
(2000)'Amino- and carboxy-terminal PEST domains mediate gastrin stabilization of rat L-histidine decarboxylase isoforms'
Fleming, J.V. and Wang, T.C.; (2000) 'Amino- and carboxy-terminal PEST domains mediate gastrin stabilization of rat L-histidine decarboxylase isoforms'. Molecular and Cellular Biology, :1463-1475 [Details]
(2000)'Gastrin is a target of the beta-catenin/TCF-4 growth-signaling pathway in a model of intestinal polyposis'
Koh, T.J., Bullita, C.J., Fleming, J.V., Vargo, A., Dockray, G.J. and Wang, T.C; (2000) 'Gastrin is a target of the beta-catenin/TCF-4 growth-signaling pathway in a model of intestinal polyposis'. Journal of Clinical Investigation, :533-539 [Details]
(1999)'Ovine arylalkylamine N-acetyl transferase (AANAT) in the pineal and pituitary glands: differences in function and regulation'
Fleming, J.V., Coon, S.L., Barret, P., Klein, D.C. and Morgan, P.J; (1999) 'Ovine arylalkylamine N-acetyl transferase (AANAT) in the pineal and pituitary glands: differences in function and regulation'. Endocrinology, :972-978 [Details]
(1998)'Partial sequences of two genes regulated by amino-acid supply identified by the use of RNA fingerprinting by arbitrarily primer PCR'
Rees, W.D., Harries, D.N. and Fleming J.V..; (1998) 'Partial sequences of two genes regulated by amino-acid supply identified by the use of RNA fingerprinting by arbitrarily primer PCR'. Journal of Nutritional Biochemistry, :136-141 [Details]
(1998)'The effect of growth arrest on differentiation and gene expression in F9 embryonal cells'
Fleming, J.V., Hay, S.M., Harries, D.N. and Rees, W.D; (1998) 'The effect of growth arrest on differentiation and gene expression in F9 embryonal cells'. Biochemical Journal, :573-579 [Details]
(1997)'The growth arrest genes gas5, gas6 and CHOP-10 (gadd153) are expressed in the mouse pre-implantation embryo'
Fleming J.V., Fontanier, N.C., Harries, D.N. and Rees,W.D.. ; (1997) 'The growth arrest genes gas5, gas6 and CHOP-10 (gadd153) are expressed in the mouse pre-implantation embryo'. Journal of molecular reproduction and development, :310-316 [Details]

Conference Publications

 YearPublication
(2013)BioIron
Fleming, J. V.,O'Driscoll, L.,Foley, L. (2013) DIFFERENTIAL CARBOHYDRATE PROCESSING OF THE WILD TYPE AND C282Y-MUTANT HFE PROTEINS BioIron   [Details]
(2013)BioIron
Foley, Louise,Fleming, J. V. (2013) OXIDATIVE STRESS DECREASES HFE PROTEIN LEVELS BioIron   [Details]
(2008)Importance of the proteases Caspase-4 and Caspase-12 in L-histidine decarboxylase
Bassani, G.A., Fleming, J.V., Sousa-Gallagher, M.J., Pico, G.A. (2008) . In: J. A. TEIXEIRA eds. Importance of the proteases Caspase-4 and Caspase-12 in L-histidine decarboxylase , pp.31-33 [Details]
(2002)Gastroenterology
Chi, A.L., Lim, S.-H., Takaishi, S., Fleming, J.V., Lee, C.-W., Wang, T.C; (2002) Gastrin transcriptionally regulates trefoil family factor 2 Gastroenterology [Details]
(2000)Journal of Reporduction and Fertility
Rassaz-Fontanier, N.C., Fleming J.V. and Rees W.D; (2000) Regulated expression of growth arrest genes in embryos developmentally retarded by culture in serum or ammonium containing media Journal of Reporduction and Fertility [Details]
(2000)Gastroenterology
Koh T, Bulitta, C.J., Fleming, J.V., Vargo, A., Dockray, G.J. and Wang, T.C. ; (2000) Gastrin is a downstream target of beta-catenin in the APC min model of intestinal adenoma Gastroenterology [Details]
(2000)Gastroenterology
Colucci, R., Fleming, J.V., Raychowdhury, R. and Wang, T.C; (2000) Feedback inhibition of histidine decarboxylase is mediated by decreased activation of GAS-RE elements Gastroenterology [Details]
(2000)Gastroenterology
Fleming, J.V., Sussman, J.S., Colucci, R., Bullita, C.J. and Wang, T.C.; (2000) Gastrin preferentially stabilizes L-histidine decarboxylase (HDC) isoforms that have been modified at both the amino- and carboxy-terminal ends Gastroenterology [Details]
(1999)Gastroenterology
Colucci, R., Fleming, J.V., Raychowdhury, R. and Wang, T.C; (1999) Feedback inhibition of histidine decarboxylase is mediated by decreased activation of GAS-RE elements Gastroenterology [Details]
(1999)Digestion
Colucci, R., Fleming, J.V., Raychowdhury, R. and Wang, T.C; (1999) Feedback inhibition of histidine decarboxylase is mediated by decreased activation of GAS-RE elements Digestion [Details]
(1999)Digestion
Fleming, J.V., Sussman, J.S. and Wang, T.C; (1999) Gastrin stimulation of L-histidine decarboxylase (HDC) activity leads to transcription independent increases in the 74kd HDC isoform Digestion [Details]
(1997)Theriongenology
Fontanier, N., Harries, D.N., Fleming, J.V. and Rees, W.D; (1997) The gene coding for the C/EBP homologous protein CHOP-10 (gadd153) is expressed in the mouse preimplantation embryo Theriongenology [Details]
(1996)Biochemical Society Transactions
Fleming J.V., Hay S.M. and Rees W.D; (1996) Effect of extracellular matrix on the expression of alkaline phosphatase activity by F9 embryonal carcinoma cells Biochemical Society Transactions [Details]
(1995)Journal of Reproduction and Fertility
Fleming J.V., Harries D.N. and Rees W.D; (1995) The gene coding for a ligand of the axl protein tyrosine kinase is expressed in murine and bovine pre-implantation embryos Journal of Reproduction and Fertility [Details]
(1995)Journal of Reproduction and Fertility
Fleming J.V., Harries D.N. and Rees W.D.; (1995) The growth arrest specific gene gas5: a marker for altered proliferation and differentiation in the in vitro cultured embryo Journal of Reproduction and Fertility [Details]

Professional Activities

Conference Contributions

 YearPublication
(2008)I Workshop Internacional em Biotecnologia, III Encontro Alfa-Valnatura, III Jornada Científica do Laboratório de Imunopatologia Keizo Asami (Lika),
Bassani, G.A., Fleming, J. V., Sousa-Gallagher, M.J. and Pico, G.A. ; (2008) Importance of the proteases Caspase-4 and Caspase-12 in L-histidine decarboxylase (Ind 45). [Conference Organising Committee Chairperson], I Workshop Internacional em Biotecnologia, III Encontro Alfa-Valnatura, III Jornada Científica do Laboratório de Imunopatologia Keizo Asami (Lika), Recife, Brazil , 05-MAY-08 - 07-MAY-08. [Details]
(2006)Institute of Molecular and Cellular Biology,
Fleming J.V; (2006) Hijacking the UPR - cellular regulation of histamine biosynthesis. [Invited Oral Presentation], Institute of Molecular and Cellular Biology, University of Porto, Portugal , 01-JAN-06 - 01-JAN-06. [Details]
(2000)Annual Workshop on Development Genetics,
Fleming J.V, Wang, T.C; (2000) Transgenic models for analyzing histidine decarboxylase expression and function. [Oral Presentation], Annual Workshop on Development Genetics, Albert Einstein College of Medicine, New York , 01-JAN-00 - 01-JAN-00. [Details]
(1997)British Neuro-Endocrine Society Annual Meeting,
Fleming J.V, Coon S.L, Barrett P, Klein D.C, Morgan P.J; (1997) Co-incident expression of NAT and MeI1a receptor mRNA in the sheep pars tuberalis. [Oral Presentation], British Neuro-Endocrine Society Annual Meeting, Cambridge, England , 01-JAN-97 - 01-JAN-97. [Details]
(1995)Scottish Initiative for Foetal and Post-natal Development, Autumn Meeting,
Fleming J.V, Harries D.N, Rees W.D; (1995) Culture conditions affect the expression of the growth arrest specific gene gas5 in the mouse embryo. [Oral Presentation], Scottish Initiative for Foetal and Post-natal Development, Autumn Meeting, Edinburgh, Scotland , 01-JAN-95 - 01-JAN-95. [Details]
(1995)Society for the study of Fertility (S.S.F) Winter Meeting,
Fleming J.V, Harries D.N, Rees W.D; (1995) The gene coding for a ligand of the axl protein tyrosine kinase is expressed in murine and bovine pre-implantation embryos. [Oral Presentation], Society for the study of Fertility (S.S.F) Winter Meeting, Oxford, England , 01-JAN-95 - 01-JAN-95. [Details]
(2005)Hijacking the UPR in the regulation of histamine biosynthesis,
Fleming, J.V; (2005) Hijacking the UPR in the regulation of histamine biosynthesis. [Oral Presentation], Hijacking the UPR in the regulation of histamine biosynthesis, IBMC, Porto, Portugal , 01-JAN-05 - 01-JAN-05. [Details]

Employment

 EmployerPositionFrom / To
University College Cork Lecturer01-MAR-06 /
Institute of Molecular Medicine, University of Lisbon, Portugal. Senior Scientist01-JAN-04 / 01-JAN-06
University of Massachusetts Medical School Research Assistant Professor01-JAN-00 / 01-JAN-04
Harvard Medical School and Massachusetts General Hospital Postdoctoral Researcher01-JAN-97 / 01-JAN-00

Education

 YearInstitutionQualificationSubject
1992University College Cork BSCBiochemistry
1997University of Aberdeen PHDUnknown

Journal Activities

 JournalRoleTo / From
Gastroenterology Referee-
Biochemical Journal Referee-
Cellular And Molecular Life Sciences Referee-
Leukemia Referee-

Teaching Activities

Teaching Interests

I teach to Biochemistry students, including on modules BC4010, BC4017 and BC4012. As a joint appointment between the Department of Biochemistry and the School of Pharmacy, I also have responsibility for co-ordinating the teaching of Biochemistry to Pharmacy student and lecture on a number of modules delivered to Pharmacy students including BC1443, BC3443, PF4109, PF4004 and PF6205.

Contact details

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School of Biological, Earth and Environmental Sciences

An Scoil Eolaíochtaí Bitheolaíocha, Domhaneolaíocha agus Comhshaoil

University College Cork, Distillery Fields, North Mall, Cork, Ireland T23 N73K

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