Research Profile

Brian McSharry

Biography

Brian McSharry graduated with a BSc in Biotechnology from the National University of Ireland, Galway (NUIG) before pursuing a PhD in Prof Gavin Wilkinson’s lab in Cardiff University studying human cytomegalovirus infection.  Following subsequent postdoctoral work at NUIG, Cardiff University and Trinity College Dublin, Brian moved to the University of Sydney in 2011 where he continued his studies into molecular mechanisms encoded by human herpesvirus infections to regulate host response  pathways. Brian returned from Australia in 2019 to take up a lecturer position in the School of Microbiology at University College Cork.

Research Interests

I am a molecular virologist with a specific interest in infections of DNA viruses, in particular, the herpesviruses; Human Cytomegalovirus (HCMV), Varicella Zoster Virus (VZV), the causative agent of chicken pox and shingles, and Herpes Simplex Virus (HSV).  I have also studied the immunomodulatory activity of adenovirus infection as well as the generation and use of replication deficient adenovirus vector systems.

Herpesviruses are important pathogens.  For example, HCMV is the leading infectious cause of congenital infection and is associated with serious disease, including sensorineural hearing loss and neurodevelopmental delays, in babies infected in utero. HCMV is also the most common infectious complication in both stem cell and solid organ transplantation, where it is associated with significant morbidity and mortality.

I am interested in how viruses control host response pathways with a focus on the identification of novel viral gene functions with immunoregulatory activity.  Natural Killer (NK) cells are known to be particularly important for effective control of herpesvirus infection. We have studied how virus infection can control NK cell responses through regulation of NK cell ligand expression on infected cells as well direct infection of this immune effector population to control their activity. I am also interested in how the interferon response regulates viral infection through the action of specific interferon stimulated genes.










Publications

Peer Reviewed Journals

 YearPublication
(2019)'Restriction of Human Cytomegalovirus Infection by Galectin-9'
Machala, EA;Avdic, S;Stern, L;Zajonc, DM;Benedict, CA;Blyth, E;Gottlieb, DJ;Abendroth, A;McSharry, BP;Slobedman, B (2019) 'Restriction of Human Cytomegalovirus Infection by Galectin-9'. Journal of Virology, 93 [DOI] [Details]
(2019)'Functional paralysis of human natural killer cells by alphaherpesviruses'
Campbell TM;McSharry BP;Steain M;Russell TA;Tscharke DC;Kennedy JJ;Slobedman B;Abendroth A; (2019) 'Functional paralysis of human natural killer cells by alphaherpesviruses'. Plos Pathogens, 15 (6) [DOI] [Details]
(2019)'Interferon-Independent Upregulation of Interferon-Stimulated Genes during Human Cytomegalovirus Infection is Dependent on IRF3 Expression'
Ashley CL;Abendroth A;McSharry BP;Slobedman B; (2019) 'Interferon-Independent Upregulation of Interferon-Stimulated Genes during Human Cytomegalovirus Infection is Dependent on IRF3 Expression'. Viruses, 11 (3) [DOI] [Details]
(2019)'Gal power: the diverse roles of galectins in regulating viral infections'
Machala EA;McSharry BP;Rouse BT;Abendroth A;Slobedman B; (2019) 'Gal power: the diverse roles of galectins in regulating viral infections'. Journal of General Virology, [DOI] [Details]
(2018)'Varicella-Zoster Virus ORF63 Protects Human Neuronal and Keratinocyte Cell Lines from Apoptosis and Changes Its Localization upon Apoptosis Induction'
Gerada, C;Steain, M;McSharry, BP;Slobedman, B;Abendroth, A (2018) 'Varicella-Zoster Virus ORF63 Protects Human Neuronal and Keratinocyte Cell Lines from Apoptosis and Changes Its Localization upon Apoptosis Induction'. Journal of Virology, 92 [DOI] [Details]
(2018)'Varicella zoster virus productively infects human natural killer cells and manipulates phenotype'
Campbell TM;McSharry BP;Steain M;Ashhurst TM;Slobedman B;Abendroth A; (2018) 'Varicella zoster virus productively infects human natural killer cells and manipulates phenotype'. Plos Pathogens, 14 (4) [DOI] [Details]
(2017)'Nuclear domain 10 components upregulated via interferon during human cytomegalovirus infection potently regulate viral infection'
Ashley CL;Glass MS;Abendroth A;McSharry BP;Slobedman B; (2017) 'Nuclear domain 10 components upregulated via interferon during human cytomegalovirus infection potently regulate viral infection'. Journal of General Virology, 98 (7) [DOI] [Details]
(2016)'Human Natural Killer cell expression of ULBP2 is associated with a mature functional phenotype'
Brennan K;McSharry BP;Keating S;Petrasca A;O'Reilly VP;Keane J;Doherty DG;Gardiner CM; (2016) 'Human Natural Killer cell expression of ULBP2 is associated with a mature functional phenotype'. Human Immunology, 77 (10) [DOI] [Details]
(2016)'Human Cytomegalovirus-Encoded Human Interleukin-10 (IL-10) Homolog Amplifies Its Immunomodulatory Potential by Upregulating Human IL-10 in Monocytes'
Avdic S;McSharry BP;Steain M;Poole E;Sinclair J;Abendroth A;Slobedman B; (2016) 'Human Cytomegalovirus-Encoded Human Interleukin-10 (IL-10) Homolog Amplifies Its Immunomodulatory Potential by Upregulating Human IL-10 in Monocytes'. Journal of Virology, 90 (8) [DOI] [Details]
(2016)'Cytomegalovirus Restructures Lipid Rafts via a US28/CDC42-Mediated Pathway, Enhancing Cholesterol Efflux from Host Cells'
Low H;Mukhamedova N;Cui HL;McSharry BP;Avdic S;Hoang A;Ditiatkovski M;Liu Y;Fu Y;Meikle PJ;Blomberg M;Polyzos KA;Miller WE;Religa P;Bukrinsky M;Soderberg-Naucler C;Slobedman B;Sviridov D; (2016) 'Cytomegalovirus Restructures Lipid Rafts via a US28/CDC42-Mediated Pathway, Enhancing Cholesterol Efflux from Host Cells'. Cell Reports, 16 (1) [DOI] [Details]
(2015)'Abrogation of the interferon response promotes more efficient human cytomegalovirus replication'
McSharry BP;Forbes SK;Avdic S;Randall RE;Wilkinson GW;Abendroth A;Slobedman B; (2015) 'Abrogation of the interferon response promotes more efficient human cytomegalovirus replication'. Journal of Virology, 89 (2) [DOI] [Details]
(2015)'Varicella-Zoster Virus and Herpes Simplex Virus 1 Differentially Modulate NKG2D Ligand Expression during Productive Infection'
Campbell TM;McSharry BP;Steain M;Slobedman B;Abendroth A; (2015) 'Varicella-Zoster Virus and Herpes Simplex Virus 1 Differentially Modulate NKG2D Ligand Expression during Productive Infection'. Journal of Virology, 89 (15) [DOI] [Details]
(2014)'Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon'
McSharry BP;Forbes SK;Cao JZ;Avdic S;Machala EA;Gottlieb DJ;Abendroth A;Slobedman B; (2014) 'Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon'. Journal of Virology, 88 (18) [DOI] [Details]
(2014)'Modulation of dendritic cell functions by viral IL-10 encoded by human cytomegalovirus'
Avdic S;McSharry BP;Slobedman B; (2014) 'Modulation of dendritic cell functions by viral IL-10 encoded by human cytomegalovirus'. Frontiers In Microbiology, 5 [DOI] [Details]
(2014)'Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation'
Fielding CA;Aicheler R;Stanton RJ;Wang EC;Han S;Seirafian S;Davies J;McSharry BP;Weekes MP;Antrobus PR;Prod'homme V;Blanchet FP;Sugrue D;Cuff S;Roberts D;Davison AJ;Lehner PJ;Wilkinson GW;Tomasec P; (2014) 'Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation'. Plos Pathogens, 10 (5) [DOI] [Details]
(2013)'Human cytomegalovirus interleukin-10 polarizes monocytes toward a deactivated M2c phenotype to repress host immune responses'
Avdic S;Cao JZ;McSharry BP;Clancy LE;Brown R;Steain M;Gottlieb DJ;Abendroth A;Slobedman B; (2013) 'Human cytomegalovirus interleukin-10 polarizes monocytes toward a deactivated M2c phenotype to repress host immune responses'. Journal of Virology, 87 (18) [DOI] [Details]
(2012)'Human cytomegalovirus UL40 signal peptide regulates cell surface expression of the NK cell ligands HLA-E and gpUL18'
Prod'homme V;Tomasec P;Cunningham C;Lemberg MK;Stanton RJ;McSharry BP;Wang EC;Cuff S;Martoglio B;Davison AJ;Braud VM;Wilkinson GW; (2012) 'Human cytomegalovirus UL40 signal peptide regulates cell surface expression of the NK cell ligands HLA-E and gpUL18'. Journal of immunology (Baltimore, Md. : 1950), 188 (6) [DOI] [Details]
(2012)'Human cytomegalovirus encoded homologs of cytokines, chemokines and their receptors: roles in immunomodulation'
McSharry BP;Avdic S;Slobedman B; (2012) 'Human cytomegalovirus encoded homologs of cytokines, chemokines and their receptors: roles in immunomodulation'. Viruses, 4 (11) [DOI] [Details]
(2011)'Innate immune genes synergize to predict increased risk of chronic disease in hepatitis C virus infection'
Dring MM;Morrison MH;McSharry BP;Guinan KJ;Hagan R; ;O'Farrelly C;Gardiner CM; (2011) 'Innate immune genes synergize to predict increased risk of chronic disease in hepatitis C virus infection'. Proceedings of The National Academy of Sciences of The United States of America, 108 (14) [DOI] [Details]
(2010)'Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication'
Stanton RJ;Baluchova K;Dargan DJ;Cunningham C;Sheehy O;Seirafian S;McSharry BP;Neale ML;Davies JA;Tomasec P;Davison AJ;Wilkinson GW; (2010) 'Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication'. Journal of Clinical Investigation, 120 (9) [DOI] [Details]
(2010)'Differential relocation and stability of PML-body components during productive human cytomegalovirus infection: detailed characterization by live-cell imaging'
Dimitropoulou P;Caswell R;McSharry BP;Greaves RF;Spandidos DA;Wilkinson GW;Sourvinos G; (2010) 'Differential relocation and stability of PML-body components during productive human cytomegalovirus infection: detailed characterization by live-cell imaging'. European Journal Of Cell Biology, 89 (10) [DOI] [Details]
(2010)'The TNF-like protein 1A-death receptor 3 pathway promotes macrophage foam cell formation in vitro'
McLaren JE;Calder CJ;McSharry BP;Sexton K;Salter RC;Singh NN;Wilkinson GW;Wang EC;Ramji DP; (2010) 'The TNF-like protein 1A-death receptor 3 pathway promotes macrophage foam cell formation in vitro'. Journal of immunology (Baltimore, Md. : 1950), 184 (10) [DOI] [Details]
(2010)'Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture'
Dargan DJ;Douglas E;Cunningham C;Jamieson F;Stanton RJ;Baluchova K;McSharry BP;Tomasec P;Emery VC;Percivalle E;Sarasini A;Gerna G;Wilkinson GW;Davison AJ; (2010) 'Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture'. Journal of General Virology, 91 (Pt 6) [DOI] [Details]
(2008)'Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation'
Lakdawala SS;Schwartz RA;Ferenchak K;Carson CT;McSharry BP;Wilkinson GW;Weitzman MD; (2008) 'Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation'. Journal of Virology, 82 (17) [DOI] [Details]
(2008)'Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function'
Stanton RJ;McSharry BP;Armstrong M;Tomasec P;Wilkinson GW; (2008) 'Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function'. Biotechniques, 45 (6) [DOI] [Details]
(2008)'Adenovirus E3/19K promotes evasion of NK cell recognition by intracellular sequestration of the NKG2D ligands major histocompatibility complex class I chain-related proteins A and B'
McSharry BP;Burgert HG;Owen DP;Stanton RJ;Prod'homme V;Sester M;Koebernick K;Groh V;Spies T;Cox S;Little AM;Wang EC;Tomasec P;Wilkinson GW; (2008) 'Adenovirus E3/19K promotes evasion of NK cell recognition by intracellular sequestration of the NKG2D ligands major histocompatibility complex class I chain-related proteins A and B'. Journal of Virology, 82 (9) [DOI] [Details]
(2008)'Modulation of natural killer cells by human cytomegalovirus'
Wilkinson GW;Tomasec P;Stanton RJ;Armstrong M;Prod'homme V;Aicheler R;McSharry BP;Rickards CR;Cochrane D;Llewellyn-Lacey S;Wang EC;Griffin CA;Davison AJ; (2008) 'Modulation of natural killer cells by human cytomegalovirus'. Journal of Clinical Virology, 41 (3) [DOI] [Details]
(2007)'Complex I binding by a virally encoded RNA regulates mitochondria-induced cell death'
Reeves MB;Davies AA;McSharry BP;Wilkinson GW;Sinclair JH; (2007) 'Complex I binding by a virally encoded RNA regulates mitochondria-induced cell death'. Science, 316 (5829) [DOI] [Details]
(2007)'Cytomegalovirus destruction of focal adhesions revealed in a high-throughput Western blot analysis of cellular protein expression'
Stanton RJ;McSharry BP;Rickards CR;Wang EC;Tomasec P;Wilkinson GW; (2007) 'Cytomegalovirus destruction of focal adhesions revealed in a high-throughput Western blot analysis of cellular protein expression'. Journal of Virology, 81 (15) [DOI] [Details]
(2007)'Adenovirus vector delivery stimulates natural killer cell recognition'
Tomasec P;Wang EC;Groh V;Spies T;McSharry BP;Aicheler RJ;Stanton RJ;Wilkinson GW; (2007) 'Adenovirus vector delivery stimulates natural killer cell recognition'. Journal of General Virology, 88 (Pt 4) [DOI] [Details]
(2005)'Characterization of a highly glycosylated form of the human cytomegalovirus HLA class I homologue gpUL18'
Griffin C;Wang EC;McSharry BP;Rickards C;Browne H;Wilkinson GW;Tomasec P; (2005) 'Characterization of a highly glycosylated form of the human cytomegalovirus HLA class I homologue gpUL18'. Journal of General Virology, 86 (Pt 11) [DOI] [Details]
(2005)'Downregulation of natural killer cell-activating ligand CD155 by human cytomegalovirus UL141'
Tomasec P;Wang EC;Davison AJ;Vojtesek B;Armstrong M;Griffin C;McSharry BP;Morris RJ;Llewellyn-Lacey S;Rickards C;Nomoto A;Sinzger C;Wilkinson GW; (2005) 'Downregulation of natural killer cell-activating ligand CD155 by human cytomegalovirus UL141'. Nature immunology, 6 (2) [DOI] [Details]
(2004)'Genetic content of wild-type human cytomegalovirus'
Dolan A;Cunningham C;Hector RD;Hassan-Walker AF;Lee L;Addison C;Dargan DJ;McGeoch DJ;Gatherer D;Emery VC;Griffiths PD;Sinzger C;McSharry BP;Wilkinson GW;Davison AJ; (2004) 'Genetic content of wild-type human cytomegalovirus'. Journal of General Virology, 85 (Pt 5) [DOI] [Details]
(2003)'Two novel spliced genes in human cytomegalovirus'
Akter P;Cunningham C;McSharry BP;Dolan A;Addison C;Dargan DJ;Hassan-Walker AF;Emery VC;Griffiths PD;Wilkinson GW;Davison AJ; (2003) 'Two novel spliced genes in human cytomegalovirus'. Journal of General Virology, 84 (Pt 5) [DOI] [Details]
(2003)'The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro'
McSharry BP;Tomasec P;Neale ML;Wilkinson GW; (2003) 'The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro'. Journal of General Virology, 84 (Pt 9) [DOI] [Details]
(2002)'UL40-mediated NK evasion during productive infection with human cytomegalovirus'
Wang EC;McSharry B;Retiere C;Tomasec P;Williams S;Borysiewicz LK;Braud VM;Wilkinson GW; (2002) 'UL40-mediated NK evasion during productive infection with human cytomegalovirus'. Proceedings of The National Academy of Sciences of The United States of America, 99 (11) [DOI] [Details]
(2001)'Human telomerase reverse transcriptase-immortalized MRC-5 and HCA2 human fibroblasts are fully permissive for human cytomegalovirus'
McSharry BP;Jones CJ;Skinner JW;Kipling D;Wilkinson GW; (2001) 'Human telomerase reverse transcriptase-immortalized MRC-5 and HCA2 human fibroblasts are fully permissive for human cytomegalovirus'. Journal of General Virology, 82 (Pt 4) [DOI] [Details]
(2000)'Surface expression of HLA-E, an inhibitor of natural killer cells, enhanced by human cytomegalovirus gpUL40'
Tomasec P;Braud VM;Rickards C;Powell MB;McSharry BP;Gadola S;Cerundolo V;Borysiewicz LK;McMichael AJ;Wilkinson GW; (2000) 'Surface expression of HLA-E, an inhibitor of natural killer cells, enhanced by human cytomegalovirus gpUL40'. Science, 287 (5455) [Details]

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School of Microbiology

Scoil na Micribhitheolaíochta

Microbiology Office, Room FSB452, 4th Floor Food Science & Technology Building, University College Cork, Cork T12 K8AF

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