IRIS publication 235379704
Differential expression of neurokinin-1 receptor by human mucosal and peripheral lymphoid cells
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TY - JOUR - Goode, T.,O'Connell, J.,Ho, W. Z.,O'Sullivan, G. C.,Collins, J. K.,Douglas, S. D.,Shanahan, F. - 2000 - May - Clinical And Diagnostic Laboratory Immunology - Differential expression of neurokinin-1 receptor by human mucosal and peripheral lymphoid cells - Validated - () - 7 - 3 - 371 - 376 - Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-l receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM), Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-IR was evident in LPMC but not in PBMC, PBL, monocytes, or MDM, However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM, This level of expression was found to represent one NK-IR mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC, These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation.Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-l receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM), Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-IR was evident in LPMC but not in PBMC, PBL, monocytes, or MDM, However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM, This level of expression was found to represent one NK-IR mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC, These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation. - 1071-412X1071-412X - ://WOS:000086873100008://WOS:000086873100008 DA - 2000/05 ER -
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@article{V235379704, = {Goode, T. and O'Connell, J. and Ho, W. Z. and O'Sullivan, G. C. and Collins, J. K. and Douglas, S. D. and Shanahan, F. }, = {2000}, = {May}, = {Clinical And Diagnostic Laboratory Immunology}, = {Differential expression of neurokinin-1 receptor by human mucosal and peripheral lymphoid cells}, = {Validated}, = {()}, = {7}, = {3}, pages = {371--376}, = {{Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-l receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM), Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-IR was evident in LPMC but not in PBMC, PBL, monocytes, or MDM, However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM, This level of expression was found to represent one NK-IR mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC, These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation.Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-l receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM), Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-IR was evident in LPMC but not in PBMC, PBL, monocytes, or MDM, However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM, This level of expression was found to represent one NK-IR mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC, These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation.}}, issn = {1071-412X1071-412X}, = {://WOS:000086873100008://WOS:000086873100008}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | Goode, T.,O'Connell, J.,Ho, W. Z.,O'Sullivan, G. C.,Collins, J. K.,Douglas, S. D.,Shanahan, F. | ||
YEAR | 2000 | ||
MONTH | May | ||
JOURNAL_CODE | Clinical And Diagnostic Laboratory Immunology | ||
TITLE | Differential expression of neurokinin-1 receptor by human mucosal and peripheral lymphoid cells | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | |||
VOLUME | 7 | ||
ISSUE | 3 | ||
START_PAGE | 371 | ||
END_PAGE | 376 | ||
ABSTRACT | Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-l receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM), Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-IR was evident in LPMC but not in PBMC, PBL, monocytes, or MDM, However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM, This level of expression was found to represent one NK-IR mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC, These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation.Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-l receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM), Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-IR was evident in LPMC but not in PBMC, PBL, monocytes, or MDM, However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM, This level of expression was found to represent one NK-IR mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC, These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation. | ||
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ISBN_ISSN | 1071-412X1071-412X | ||
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URL | ://WOS:000086873100008://WOS:000086873100008 | ||
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