IRIS publication 280546861
Asymptomatic carriage of Clostridium difficile in an Irish continuing care institution for the elderly: prevalence and characteristics
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TY - JOUR - Ryan, J.,Murphy, C.,Twomey, C.,Paul Ross, R.,Rea, M. C.,MacSharry, J.,Sheil, B.,Shanahan, F. - 2010 - June - Irish Journal of Medical Science - Asymptomatic carriage of Clostridium difficile in an Irish continuing care institution for the elderly: prevalence and characteristics - Validated - () - 179 - 2 - 245 - 250 - INTRODUCTION: Clostridium difficile is an increasing cause of nosocomial diarrhoea and colitis. The aim of this study was to identify the prevalence and characteristics of asymptomatic C. difficile carriage in a continuing care institution for the elderly. METHODS: Stool samples were collected from 100 asymptomatic patients, whose median age was 83 years. Samples were tested for C. difficile using traditional culturing methods, 16s rDNA and 16s-23s intergenic spacer (IGS) rDNA sequencing, and analysed for toxin production and toxin genes. RESULTS: The prevalence of C. difficile carriage was 10/100 (10%) following culture and 16s and IGS sequencing. An additional seven isolates, initially identified as C. difficile, were subsequently identified by IGS rDNA sequencing as C. sordellii of the 10% that tested positive for C. difficile, seven tested positive for toxin A and B. A significant number of C. difficile carriers had recent antibiotic exposure compared with non-carriers, P = 0.046. CONCLUSION: The prevalence of asymptomatic C. difficile carriage in this institution was 10%, 7% of which were toxin positive. This study underscores the importance of increased vigilance for C. difficile using microbial and molecular methodology and identifies patients at increased risk following antibiotic administration.INTRODUCTION: Clostridium difficile is an increasing cause of nosocomial diarrhoea and colitis. The aim of this study was to identify the prevalence and characteristics of asymptomatic C. difficile carriage in a continuing care institution for the elderly. METHODS: Stool samples were collected from 100 asymptomatic patients, whose median age was 83 years. Samples were tested for C. difficile using traditional culturing methods, 16s rDNA and 16s-23s intergenic spacer (IGS) rDNA sequencing, and analysed for toxin production and toxin genes. RESULTS: The prevalence of C. difficile carriage was 10/100 (10%) following culture and 16s and IGS sequencing. An additional seven isolates, initially identified as C. difficile, were subsequently identified by IGS rDNA sequencing as C. sordellii of the 10% that tested positive for C. difficile, seven tested positive for toxin A and B. A significant number of C. difficile carriers had recent antibiotic exposure compared with non-carriers, P = 0.046. CONCLUSION: The prevalence of asymptomatic C. difficile carriage in this institution was 10%, 7% of which were toxin positive. This study underscores the importance of increased vigilance for C. difficile using microbial and molecular methodology and identifies patients at increased risk following antibiotic administration. - 0021-12650021-1265 DA - 2010/06 ER -
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@article{V280546861, = {Ryan, J. and Murphy, C. and Twomey, C. and Paul Ross, R. and Rea, M. C. and MacSharry, J. and Sheil, B. and Shanahan, F. }, = {2010}, = {June}, = {Irish Journal of Medical Science}, = {Asymptomatic carriage of Clostridium difficile in an Irish continuing care institution for the elderly: prevalence and characteristics}, = {Validated}, = {()}, = {179}, = {2}, pages = {245--250}, = {{INTRODUCTION: Clostridium difficile is an increasing cause of nosocomial diarrhoea and colitis. The aim of this study was to identify the prevalence and characteristics of asymptomatic C. difficile carriage in a continuing care institution for the elderly. METHODS: Stool samples were collected from 100 asymptomatic patients, whose median age was 83 years. Samples were tested for C. difficile using traditional culturing methods, 16s rDNA and 16s-23s intergenic spacer (IGS) rDNA sequencing, and analysed for toxin production and toxin genes. RESULTS: The prevalence of C. difficile carriage was 10/100 (10%) following culture and 16s and IGS sequencing. An additional seven isolates, initially identified as C. difficile, were subsequently identified by IGS rDNA sequencing as C. sordellii of the 10% that tested positive for C. difficile, seven tested positive for toxin A and B. A significant number of C. difficile carriers had recent antibiotic exposure compared with non-carriers, P = 0.046. CONCLUSION: The prevalence of asymptomatic C. difficile carriage in this institution was 10%, 7% of which were toxin positive. This study underscores the importance of increased vigilance for C. difficile using microbial and molecular methodology and identifies patients at increased risk following antibiotic administration.INTRODUCTION: Clostridium difficile is an increasing cause of nosocomial diarrhoea and colitis. The aim of this study was to identify the prevalence and characteristics of asymptomatic C. difficile carriage in a continuing care institution for the elderly. METHODS: Stool samples were collected from 100 asymptomatic patients, whose median age was 83 years. Samples were tested for C. difficile using traditional culturing methods, 16s rDNA and 16s-23s intergenic spacer (IGS) rDNA sequencing, and analysed for toxin production and toxin genes. RESULTS: The prevalence of C. difficile carriage was 10/100 (10%) following culture and 16s and IGS sequencing. An additional seven isolates, initially identified as C. difficile, were subsequently identified by IGS rDNA sequencing as C. sordellii of the 10% that tested positive for C. difficile, seven tested positive for toxin A and B. A significant number of C. difficile carriers had recent antibiotic exposure compared with non-carriers, P = 0.046. CONCLUSION: The prevalence of asymptomatic C. difficile carriage in this institution was 10%, 7% of which were toxin positive. This study underscores the importance of increased vigilance for C. difficile using microbial and molecular methodology and identifies patients at increased risk following antibiotic administration.}}, issn = {0021-12650021-1265}, source = {IRIS} }
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AUTHORS | Ryan, J.,Murphy, C.,Twomey, C.,Paul Ross, R.,Rea, M. C.,MacSharry, J.,Sheil, B.,Shanahan, F. | ||
YEAR | 2010 | ||
MONTH | June | ||
JOURNAL_CODE | Irish Journal of Medical Science | ||
TITLE | Asymptomatic carriage of Clostridium difficile in an Irish continuing care institution for the elderly: prevalence and characteristics | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | |||
VOLUME | 179 | ||
ISSUE | 2 | ||
START_PAGE | 245 | ||
END_PAGE | 250 | ||
ABSTRACT | INTRODUCTION: Clostridium difficile is an increasing cause of nosocomial diarrhoea and colitis. The aim of this study was to identify the prevalence and characteristics of asymptomatic C. difficile carriage in a continuing care institution for the elderly. METHODS: Stool samples were collected from 100 asymptomatic patients, whose median age was 83 years. Samples were tested for C. difficile using traditional culturing methods, 16s rDNA and 16s-23s intergenic spacer (IGS) rDNA sequencing, and analysed for toxin production and toxin genes. RESULTS: The prevalence of C. difficile carriage was 10/100 (10%) following culture and 16s and IGS sequencing. An additional seven isolates, initially identified as C. difficile, were subsequently identified by IGS rDNA sequencing as C. sordellii of the 10% that tested positive for C. difficile, seven tested positive for toxin A and B. A significant number of C. difficile carriers had recent antibiotic exposure compared with non-carriers, P = 0.046. CONCLUSION: The prevalence of asymptomatic C. difficile carriage in this institution was 10%, 7% of which were toxin positive. This study underscores the importance of increased vigilance for C. difficile using microbial and molecular methodology and identifies patients at increased risk following antibiotic administration.INTRODUCTION: Clostridium difficile is an increasing cause of nosocomial diarrhoea and colitis. The aim of this study was to identify the prevalence and characteristics of asymptomatic C. difficile carriage in a continuing care institution for the elderly. METHODS: Stool samples were collected from 100 asymptomatic patients, whose median age was 83 years. Samples were tested for C. difficile using traditional culturing methods, 16s rDNA and 16s-23s intergenic spacer (IGS) rDNA sequencing, and analysed for toxin production and toxin genes. RESULTS: The prevalence of C. difficile carriage was 10/100 (10%) following culture and 16s and IGS sequencing. An additional seven isolates, initially identified as C. difficile, were subsequently identified by IGS rDNA sequencing as C. sordellii of the 10% that tested positive for C. difficile, seven tested positive for toxin A and B. A significant number of C. difficile carriers had recent antibiotic exposure compared with non-carriers, P = 0.046. CONCLUSION: The prevalence of asymptomatic C. difficile carriage in this institution was 10%, 7% of which were toxin positive. This study underscores the importance of increased vigilance for C. difficile using microbial and molecular methodology and identifies patients at increased risk following antibiotic administration. | ||
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ISBN_ISSN | 0021-12650021-1265 | ||
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