IRIS publication 722468
Enhancing Bile Tolerance Improves Survival and Persistence of Bifidobacterium and Lactococcus In The Murine Gastrointestinal Tract
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TY - JOUR - Watson, D, Sleator, RD, Hill, C, Gahan, CGM - 2008 - August - BMC Microbiology - Enhancing Bile Tolerance Improves Survival and Persistence of Bifidobacterium and Lactococcus In The Murine Gastrointestinal Tract - Validated - () - 8 - NA - Results: In vitro bile tolerance of both strains was significantly enhanced (P < 0.001), following heterologous expression of the Listeria monocytogenes bile resistance mechanism BilE. Strains harbouring bilE were also recovered at significantly higher levels (P < 0.001), than control strains from the faeces and intestines of mice (n = 5), following oral inoculation. Furthermore, a B. breve strain expressing bilE demonstrated increased efficacy relative to the wild-type strain in reducing oral L. monocytogenes infection in mice.. Conclusion: Collectively the data indicates that bile tolerance can be enhanced in Bifidobacterium and Lactococcus species through rational. Background: The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS) organism Lactococcus lactis NZ9000.. - DOI 10.1186/1471-2180-8-176 DA - 2008/08 ER -
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@article{V722468, = {Watson, D and Sleator, RD and Hill, C and Gahan, CGM }, = {2008}, = {August}, = {BMC Microbiology}, = {Enhancing Bile Tolerance Improves Survival and Persistence of Bifidobacterium and Lactococcus In The Murine Gastrointestinal Tract}, = {Validated}, = {()}, = {8}, = {NA}, = {{Results: In vitro bile tolerance of both strains was significantly enhanced (P < 0.001), following heterologous expression of the Listeria monocytogenes bile resistance mechanism BilE. Strains harbouring bilE were also recovered at significantly higher levels (P < 0.001), than control strains from the faeces and intestines of mice (n = 5), following oral inoculation. Furthermore, a B. breve strain expressing bilE demonstrated increased efficacy relative to the wild-type strain in reducing oral L. monocytogenes infection in mice.. Conclusion: Collectively the data indicates that bile tolerance can be enhanced in Bifidobacterium and Lactococcus species through rational. Background: The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS) organism Lactococcus lactis NZ9000..}}, = {DOI 10.1186/1471-2180-8-176}, source = {IRIS} }
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AUTHORS | Watson, D, Sleator, RD, Hill, C, Gahan, CGM | ||
YEAR | 2008 | ||
MONTH | August | ||
JOURNAL_CODE | BMC Microbiology | ||
TITLE | Enhancing Bile Tolerance Improves Survival and Persistence of Bifidobacterium and Lactococcus In The Murine Gastrointestinal Tract | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
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VOLUME | 8 | ||
ISSUE | NA | ||
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ABSTRACT | Results: In vitro bile tolerance of both strains was significantly enhanced (P < 0.001), following heterologous expression of the Listeria monocytogenes bile resistance mechanism BilE. Strains harbouring bilE were also recovered at significantly higher levels (P < 0.001), than control strains from the faeces and intestines of mice (n = 5), following oral inoculation. Furthermore, a B. breve strain expressing bilE demonstrated increased efficacy relative to the wild-type strain in reducing oral L. monocytogenes infection in mice.. Conclusion: Collectively the data indicates that bile tolerance can be enhanced in Bifidobacterium and Lactococcus species through rational. Background: The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS) organism Lactococcus lactis NZ9000.. | ||
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DOI_LINK | DOI 10.1186/1471-2180-8-176 | ||
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