TY  - JOUR
  - Motherway, MO,O'Driscoll, J,Fitzgerald, GF,van Sinderen, D
  - 2009
  - May
  - Microbial Biotechnology
  - Overcoming the restriction barrier to plasmid transformation and targeted mutagenesis in Bifidobacterium breve UCC2003
  - Validated
  - Altmetric: 1 ()
  - SITE-SPECIFIC ENDONUCLEASE SEQUENCE-ANALYSIS DNA METHYLTRANSFERASES MODIFICATION SYSTEM LACTOCOCCUS-LACTIS LONGUM CONSTRUCTION STRAINS CLONING GENES
  - 2
  - 321
  - 332
  - In silico analysis of the Bifidobacterium breve UCC2003 genome predicted two distinct loci, which encode three different restriction/modification systems, each comprising a modification methylase and a restriction endonuclease. Based on sequence homology and observed protection against restriction we conclude that the first restriction endonuclease, designated BbrI, is an isoschizomer of BbeI, the second, BbrII, is a neoschizomer of SalI, while the third, BbrIII, is an isoschizomer of PstI. Expression of each of the B. breve UCC2003 methylase-encoding genes in B. breve JCM 7017 established that BbrII and BbrIII are active and restrict incoming DNA. By exploiting knowledge on restriction/modification in B. breve UCC2003 we successfully increased the transformation efficiency to a level that allows the reliable generation of mutants by homologous recombination using a non-replicative plasmid.
  - 10.1111/j.1751-7915.2008.00071.x
DA  - 2009/05
ER  - 

@article{V243943095,
   = {Motherway,  MO and O'Driscoll,  J and Fitzgerald,  GF and van Sinderen,  D },
   = {2009},
   = {May},
   = {Microbial Biotechnology},
   = {Overcoming the restriction barrier to plasmid transformation and targeted mutagenesis in Bifidobacterium breve UCC2003},
   = {Validated},
   = {Altmetric: 1 ()},
   = {SITE-SPECIFIC ENDONUCLEASE SEQUENCE-ANALYSIS DNA METHYLTRANSFERASES MODIFICATION SYSTEM LACTOCOCCUS-LACTIS LONGUM CONSTRUCTION STRAINS CLONING GENES},
   = {2},
  pages = {321--332},
   = {{In silico analysis of the Bifidobacterium breve UCC2003 genome predicted two distinct loci, which encode three different restriction/modification systems, each comprising a modification methylase and a restriction endonuclease. Based on sequence homology and observed protection against restriction we conclude that the first restriction endonuclease, designated BbrI, is an isoschizomer of BbeI, the second, BbrII, is a neoschizomer of SalI, while the third, BbrIII, is an isoschizomer of PstI. Expression of each of the B. breve UCC2003 methylase-encoding genes in B. breve JCM 7017 established that BbrII and BbrIII are active and restrict incoming DNA. By exploiting knowledge on restriction/modification in B. breve UCC2003 we successfully increased the transformation efficiency to a level that allows the reliable generation of mutants by homologous recombination using a non-replicative plasmid.}},
   = {10.1111/j.1751-7915.2008.00071.x},
  source = {IRIS}
}