TY  - JOUR
  - Vegge, CS,Vogensen, FK,McGrath, S,Neve, H,van Sinderen, D,Brondsted, L
  - 2006
  - January
  - Journal of Bacteriology
  - Identification of the lower baseplate protein as the antireceptor of the temperate lactococcal bacteriophages TP901-1 and Tuc2009
  - Validated
  - ()
  - GRAM-POSITIVE BACTERIA PHAGE TP901-1 COMPARATIVE GENOMICS MOLECULAR CHARACTERIZATION LACTIC STREPTOCOCCI DNA-SEQUENCE TAIL GENE RECEPTOR SPECIFICITY
  - 188
  - 55
  - 63
  - The first step in the infection process of tailed phages is recognition and binding to the host receptor. This interaction is mediated by the phage antireceptor located in the distal tail structure. The temperate Lactococcus lactis phage TP901-1 belongs to the P335 species of the Siphoviridae family, which also includes the related phage Tuc2009. The distal tail structure of TP901-1 is well characterized and contains a double-disk baseplate and a central tail fiber. The structural tail proteins of TP901-1 and Tuc2009 are highly similar, but the phages have different host ranges and must therefore encode different antireceptors. In order to identify the antireceptors of TP901-1 and Tuc2009, a chimeric phage was generated in which the gene encoding the TP901-1 lower baseplate protein (bppL(TP901-1)) was exchanged with the analogous gene (orf53(2009)) of phage Tuc2009. The chimeric phage (TP901-1C) infected the Tuc2009 host strain efficiently and thus displayed an altered host range compared to TP901-1. Genomic analysis and sequencing verified that TP901-1C is a TP901-1 derivative containing the orf53(2009) gene in exchange for bppL(TP901-1); however, a new sequence in the late promoter region was also discovered. Protein analysis confirmed that TP901-1C contains ORF53,009 and not the lower baseplate protein BPLTP901-1 and it was concluded that Bpp(TP901-1) and ORF53(2009) constitute antireceptor proteins of TP901-1 and Tuc2009, respectively. Electron micrographs revealed altered baseplate morphology of TP901-IC compared to that of the parental phage.
  - DOI 10.1128/JB.1.55-63.2006
DA  - 2006/01
ER  - 

@article{V43336754,
   = {Vegge,  CS and Vogensen,  FK and McGrath,  S and Neve,  H and van Sinderen,  D and Brondsted,  L },
   = {2006},
   = {January},
   = {Journal of Bacteriology},
   = {Identification of the lower baseplate protein as the antireceptor of the temperate lactococcal bacteriophages TP901-1 and Tuc2009},
   = {Validated},
   = {()},
   = {GRAM-POSITIVE BACTERIA PHAGE TP901-1 COMPARATIVE GENOMICS MOLECULAR CHARACTERIZATION LACTIC STREPTOCOCCI DNA-SEQUENCE TAIL GENE RECEPTOR SPECIFICITY},
   = {188},
  pages = {55--63},
   = {{The first step in the infection process of tailed phages is recognition and binding to the host receptor. This interaction is mediated by the phage antireceptor located in the distal tail structure. The temperate Lactococcus lactis phage TP901-1 belongs to the P335 species of the Siphoviridae family, which also includes the related phage Tuc2009. The distal tail structure of TP901-1 is well characterized and contains a double-disk baseplate and a central tail fiber. The structural tail proteins of TP901-1 and Tuc2009 are highly similar, but the phages have different host ranges and must therefore encode different antireceptors. In order to identify the antireceptors of TP901-1 and Tuc2009, a chimeric phage was generated in which the gene encoding the TP901-1 lower baseplate protein (bppL(TP901-1)) was exchanged with the analogous gene (orf53(2009)) of phage Tuc2009. The chimeric phage (TP901-1C) infected the Tuc2009 host strain efficiently and thus displayed an altered host range compared to TP901-1. Genomic analysis and sequencing verified that TP901-1C is a TP901-1 derivative containing the orf53(2009) gene in exchange for bppL(TP901-1); however, a new sequence in the late promoter region was also discovered. Protein analysis confirmed that TP901-1C contains ORF53,009 and not the lower baseplate protein BPLTP901-1 and it was concluded that Bpp(TP901-1) and ORF53(2009) constitute antireceptor proteins of TP901-1 and Tuc2009, respectively. Electron micrographs revealed altered baseplate morphology of TP901-IC compared to that of the parental phage.}},
   = {DOI 10.1128/JB.1.55-63.2006},
  source = {IRIS}
}