TY  - JOUR
  - Ventura, M,Zhang, ZD,Cronin, M,Canchaya, C,Kenny, JG,Fitzgerald, GF,van Sinderen, D
  - 2005
  - May
  - Journal of Bacteriology
  - The ClgR protein regulates transcription of the clpP operon in Bifidobacterium breve UCC 2003
  - Validated
  - ()
  - LACTIC-ACID BACTERIA HEAT-SHOCK RESPONSE STREPTOMYCES-LIVIDANS ESCHERICHIA-COLI MOLECULAR CHAPERONES BACILLUS-SUBTILIS GENE-EXPRESSION UCC 2003 IDENTIFICATION STRESS
  - 187
  - 8411
  - 8426
  - Five clp genes (clpC, clpB, clpPl, clpP2, and clpX), representing chaperone- and protease-encoding genes, were previously identified in Bifidobacterium breve UCC 2003. In the present study, we characterize the B. breve UCC 2003 clpP locus, which consists of two paralogous genes, designated clwpP1 and clpP2, whose deduced protein products display significant similarity to characterized CIpP peptidases. Transcriptional analyses showed that the clpP1 and clpP2 genes are transcribed in response to moderate heat shock as a bicistronic unit with a single promoter. The role of a clgR homologue, known to control the regulation of clpP gene expression in Streptomyces lividans and Corynebacterium glutamicum, was investigated by gel mobility shift assays and DNase I footprint experiments. We show that ClgR, which in its purified form appears to exist as a dimer, requires a proteinaceous cofactor to assist in specific binding to a 30-bp region of the clpP promoter region. In pull-down experiments, a 56-kDa protein copurified with ClgR, providing evidence that the two proteins also interact in vivo and that the copurified protein represents the cofactor required for ClgR activity. The prediction of the ClgR three-dimensional structure provides further insights into the binding mode of this protein to the clpPI promoter region and highlights the key amino acid residues believed to be involved in the protein-DNA interaction.
  - DOI 10.1128/JB.187.24.8411-8426.2005
DA  - 2005/05
ER  - 

@article{V43336989,
   = {Ventura,  M and Zhang,  ZD and Cronin,  M and Canchaya,  C and Kenny,  JG and Fitzgerald,  GF and van Sinderen,  D },
   = {2005},
   = {May},
   = {Journal of Bacteriology},
   = {The ClgR protein regulates transcription of the clpP operon in Bifidobacterium breve UCC 2003},
   = {Validated},
   = {()},
   = {LACTIC-ACID BACTERIA HEAT-SHOCK RESPONSE STREPTOMYCES-LIVIDANS ESCHERICHIA-COLI MOLECULAR CHAPERONES BACILLUS-SUBTILIS GENE-EXPRESSION UCC 2003 IDENTIFICATION STRESS},
   = {187},
  pages = {8411--8426},
   = {{Five clp genes (clpC, clpB, clpPl, clpP2, and clpX), representing chaperone- and protease-encoding genes, were previously identified in Bifidobacterium breve UCC 2003. In the present study, we characterize the B. breve UCC 2003 clpP locus, which consists of two paralogous genes, designated clwpP1 and clpP2, whose deduced protein products display significant similarity to characterized CIpP peptidases. Transcriptional analyses showed that the clpP1 and clpP2 genes are transcribed in response to moderate heat shock as a bicistronic unit with a single promoter. The role of a clgR homologue, known to control the regulation of clpP gene expression in Streptomyces lividans and Corynebacterium glutamicum, was investigated by gel mobility shift assays and DNase I footprint experiments. We show that ClgR, which in its purified form appears to exist as a dimer, requires a proteinaceous cofactor to assist in specific binding to a 30-bp region of the clpP promoter region. In pull-down experiments, a 56-kDa protein copurified with ClgR, providing evidence that the two proteins also interact in vivo and that the copurified protein represents the cofactor required for ClgR activity. The prediction of the ClgR three-dimensional structure provides further insights into the binding mode of this protein to the clpPI promoter region and highlights the key amino acid residues believed to be involved in the protein-DNA interaction.}},
   = {DOI 10.1128/JB.187.24.8411-8426.2005},
  source = {IRIS}
}