TY  - JOUR
  - O'Connell Motherway M, Zomer A, Leahy SC, Reunanen J, Bottacini F, Claesson MJ, O'Brien F, Flynn K, Casey PG, Munoz JA, Kearney B, Houston AM, O'Mahony C, Higgins DG, Shanahan F, Palva A, de Vos WM, Fitzgerald GF, Ventura M, O'Toole PW, van Sinderen D
  - 2011
  - July
  - Proceedings of The National Academy of Sciences of The United States of America
  - Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor.
  - Validated
  - Altmetric: 1 ()
  - 108
  - 27
  - 11217
  - 11222
  - Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tad(2003)." Mutational analysis demonstrated that the tad(2003) gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.
  - 10.1073/pnas.1105380108
DA  - 2011/07
ER  - 

@article{V112824891,
   = {O'Connell Motherway M,  Zomer A and  Leahy SC,  Reunanen J and  Bottacini F,  Claesson MJ and  O'Brien F,  Flynn K and  Casey PG,  Munoz JA and  Kearney B,  Houston AM and  O'Mahony C,  Higgins DG and  Shanahan F,  Palva A and  de Vos WM,  Fitzgerald GF and  Ventura M,  O'Toole PW and  van Sinderen D },
   = {2011},
   = {July},
   = {Proceedings of The National Academy of Sciences of The United States of America},
   = {Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor.},
   = {Validated},
   = {Altmetric: 1 ()},
   = {108},
   = {27},
  pages = {11217--11222},
   = {{Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tad(2003)." Mutational analysis demonstrated that the tad(2003) gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.}},
   = {10.1073/pnas.1105380108},
  source = {IRIS}
}