TY  - JOUR
  - Wilson, CM,Aggio, RBM,O'Toole, PW,Villas-Boas, S,Tannock, GW
  - 2012
  - January
  - Journal of Bacteriology
  - Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23
  - Validated
  - ()
  - OXIDATIVE STRESS MICROBIAL-CELLS BACTERIA THIOREDOXIN GENE
  - 194
  - 1743
  - 1746
  - Autoinducer-2 (AI-2)-mediated quorum sensing has been extensively studied in relation to the regulation of microbial behavior. There are, however, two potential roles for the AI-2 synthase (LuxS). The first is in the production of AI-2 and the second is as an enzyme in the activated methyl cycle, where it catalyzes the conversion of S-ribosylhomocysteine to homocysteine. The by-product of the reaction catalyzed by LuxS is (S)-4,5-dihydroxy-2,3-pentanedione, which spontaneously forms the furanones known collectively as AI-2. The mammalian gut contains a complex collection of bacterial species so a method of interspecies communication might influence community structure and function. Lactobacillus reuteri 100-23 is an autochthonous inhabitant of the rodent forestomach, where it adheres to the nonsecretory epithelium, forming a biofilm. Microarray comparisons of gene expression profiles of the L. reuteri 100-23 wild type and a luxS mutant under different culture conditions revealed altered transcription of genes encoding proteins associated with cysteine biosynthesis/oxidative stress response, urease activity, and sortase-dependent proteins. Metabolomic analysis showed that the luxS mutation affected cellular levels of fermentation products, fatty acids and amino acids. Cell density-dependent changes (log phase versus stationary phase growth) in gene transcription were not detected, indicating that AI-2 was unlikely to be involved in gene regulation mediated by quorum sensing in L. reuteri 100-23.
  - DOI 10.1128/JB.06318-11
DA  - 2012/01
ER  - 

@article{V160747965,
   = {Wilson,  CM and Aggio,  RBM and O'Toole,  PW and Villas-Boas,  S and Tannock,  GW },
   = {2012},
   = {January},
   = {Journal of Bacteriology},
   = {Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23},
   = {Validated},
   = {()},
   = {OXIDATIVE STRESS MICROBIAL-CELLS BACTERIA THIOREDOXIN GENE},
   = {194},
  pages = {1743--1746},
   = {{Autoinducer-2 (AI-2)-mediated quorum sensing has been extensively studied in relation to the regulation of microbial behavior. There are, however, two potential roles for the AI-2 synthase (LuxS). The first is in the production of AI-2 and the second is as an enzyme in the activated methyl cycle, where it catalyzes the conversion of S-ribosylhomocysteine to homocysteine. The by-product of the reaction catalyzed by LuxS is (S)-4,5-dihydroxy-2,3-pentanedione, which spontaneously forms the furanones known collectively as AI-2. The mammalian gut contains a complex collection of bacterial species so a method of interspecies communication might influence community structure and function. Lactobacillus reuteri 100-23 is an autochthonous inhabitant of the rodent forestomach, where it adheres to the nonsecretory epithelium, forming a biofilm. Microarray comparisons of gene expression profiles of the L. reuteri 100-23 wild type and a luxS mutant under different culture conditions revealed altered transcription of genes encoding proteins associated with cysteine biosynthesis/oxidative stress response, urease activity, and sortase-dependent proteins. Metabolomic analysis showed that the luxS mutation affected cellular levels of fermentation products, fatty acids and amino acids. Cell density-dependent changes (log phase versus stationary phase growth) in gene transcription were not detected, indicating that AI-2 was unlikely to be involved in gene regulation mediated by quorum sensing in L. reuteri 100-23.}},
   = {DOI 10.1128/JB.06318-11},
  source = {IRIS}
}