TY  - JOUR
  - O'Sullivan, O,Coakley, M,Lakshminarayanan, B,Claesson, MJ,Stanton, C,O'Toole, PW,Ross, RP,ELDERMET Consortium
  - 2011
  - August
  - Journal of Applied Microbiology
  - Correlation of rRNA gene amplicon pyrosequencing and bacterial culture for microbial compositional analysis of faecal samples from elderly Irish subjects
  - Validated
  - WOS: 18 ()
  - bacterial culture bifidobacteria elderly Irish lactobacilli sequencing HUMAN INTESTINAL MICROBIOTA GUT MICROBIOTA BIFIDOBACTERIA DIVERSITY AGE COMMUNITIES FLORA SUPPLEMENTATION IDENTIFICATION LACTOBACILLUS
  - 111
  - 467
  - 473
  - Aims: The aim of this investigation was to establish the degree of correlation between measurements from culture-dependent microbiological techniques and from next generation sequencing technologies.Methods and Results: Data generated by both techniques were collected from faecal samples from 185 elderly Irish people involved in the ongoing ELDER-MET study (http://eldermet.ucc.ie). The results for three groups of intestinal bacteria were compared. Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were enumerated on selective media through culture-dependent techniques, whereas proportions of these bacteria were determined through sequencing technology against the background of other bacteria. The Spearman's rank correlation coefficient determined a good correlation between results from culture-dependent microbiology and culture-independent techniques for all three bacterial groups assessed (correlation coefficients for Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were 0.380, 0.366 and 0.437, respectively).Conclusion: Correlation between the two methods implies that a single method is capable of profiling intestinal Bifidobacterium, Lactobacillus and Enterobacteriaceae populations. However, both methods have advantages that justify their use in tandem.Significance and Impact of the Study: This is the first extensive study to compare bacterial counts from culture-dependent microbiological techniques and from next generation sequencing technologies.
  - 10.1111/j.1365-2672.2011.05067.x
DA  - 2011/08
ER  - 

@article{V243944085,
   = {O'Sullivan,  O and Coakley,  M and Lakshminarayanan,  B and Claesson,  MJ and Stanton,  C and O'Toole,  PW and Ross,  RP and ELDERMET Consortium },
   = {2011},
   = {August},
   = {Journal of Applied Microbiology},
   = {Correlation of rRNA gene amplicon pyrosequencing and bacterial culture for microbial compositional analysis of faecal samples from elderly Irish subjects},
   = {Validated},
   = {WOS: 18 ()},
   = {bacterial culture bifidobacteria elderly Irish lactobacilli sequencing HUMAN INTESTINAL MICROBIOTA GUT MICROBIOTA BIFIDOBACTERIA DIVERSITY AGE COMMUNITIES FLORA SUPPLEMENTATION IDENTIFICATION LACTOBACILLUS},
   = {111},
  pages = {467--473},
   = {{Aims: The aim of this investigation was to establish the degree of correlation between measurements from culture-dependent microbiological techniques and from next generation sequencing technologies.Methods and Results: Data generated by both techniques were collected from faecal samples from 185 elderly Irish people involved in the ongoing ELDER-MET study (http://eldermet.ucc.ie). The results for three groups of intestinal bacteria were compared. Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were enumerated on selective media through culture-dependent techniques, whereas proportions of these bacteria were determined through sequencing technology against the background of other bacteria. The Spearman's rank correlation coefficient determined a good correlation between results from culture-dependent microbiology and culture-independent techniques for all three bacterial groups assessed (correlation coefficients for Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were 0.380, 0.366 and 0.437, respectively).Conclusion: Correlation between the two methods implies that a single method is capable of profiling intestinal Bifidobacterium, Lactobacillus and Enterobacteriaceae populations. However, both methods have advantages that justify their use in tandem.Significance and Impact of the Study: This is the first extensive study to compare bacterial counts from culture-dependent microbiological techniques and from next generation sequencing technologies.}},
   = {10.1111/j.1365-2672.2011.05067.x},
  source = {IRIS}
}