TY  - JOUR
  - Wilson, CM,Loach, D,Lawley, B,Bell, T,Sims, IM,O'Toole, PW,Zomer, A,Tannock, GW
  - 2014
  - October
  - Applied and Environmental Microbiology
  - Lactobacillus reuteri 100-23 Modulates Urea Hydrolysis in the Murine Stomach
  - Validated
  - WOS: 19 ()
  - MOUSE GASTROINTESTINAL-TRACT VIVO EXPRESSION TECHNOLOGY DNA-MICROARRAY DATA GENE-EXPRESSION HELICOBACTER-PYLORI ACID RESISTANCE SMALL-BOWEL REQUIREMENTS FORESTOMACH GLUTAMATE
  - 80
  - 6104
  - 6113
  - Comparisons of in vivo (mouse stomach) and in vitro (laboratory culture) transcriptomes of Lactobacillus reuteri strain 100-23 were made by microarray analysis. These comparisons revealed the upregulation of genes associated with acid tolerance, including urease production, in the mouse stomach. Inactivation of the ureC gene reduced the acid tolerance of strain 100-23 in vitro, and the mutant was outcompeted by the wild type in the gut of ex-Lactobacillus-free mice. Urine analysis showed that stable iso-tope-labeled urea, administered by gavage, was metabolized to a greater extent in Lactobacillus-free mice than animals colonized by strain 100-23. This surprising observation was associated with higher levels of urease activity and fecal-type bacteria in the stomach digesta of Lactobacillus-free mice. Despite the modulation of urea hydrolysis in the stomach, recycling of urea nitrogen in the murine host was not affected since the essential amino acid isoleucine, labeled with a stable isotope, was detected in the livers of both Lactobacillus-free and 100-23-colonized animals. Therefore, our experiments reveal a new and unexpected impact of Lactobacillus colonization on urea hydrolysis in the murine gut.
  - 10.1128/AEM.01876-14
DA  - 2014/10
ER  - 

@article{V279268917,
   = {Wilson,  CM and Loach,  D and Lawley,  B and Bell,  T and Sims,  IM and O'Toole,  PW and Zomer,  A and Tannock,  GW },
   = {2014},
   = {October},
   = {Applied and Environmental Microbiology},
   = {Lactobacillus reuteri 100-23 Modulates Urea Hydrolysis in the Murine Stomach},
   = {Validated},
   = {WOS: 19 ()},
   = {MOUSE GASTROINTESTINAL-TRACT VIVO EXPRESSION TECHNOLOGY DNA-MICROARRAY DATA GENE-EXPRESSION HELICOBACTER-PYLORI ACID RESISTANCE SMALL-BOWEL REQUIREMENTS FORESTOMACH GLUTAMATE},
   = {80},
  pages = {6104--6113},
   = {{Comparisons of in vivo (mouse stomach) and in vitro (laboratory culture) transcriptomes of Lactobacillus reuteri strain 100-23 were made by microarray analysis. These comparisons revealed the upregulation of genes associated with acid tolerance, including urease production, in the mouse stomach. Inactivation of the ureC gene reduced the acid tolerance of strain 100-23 in vitro, and the mutant was outcompeted by the wild type in the gut of ex-Lactobacillus-free mice. Urine analysis showed that stable iso-tope-labeled urea, administered by gavage, was metabolized to a greater extent in Lactobacillus-free mice than animals colonized by strain 100-23. This surprising observation was associated with higher levels of urease activity and fecal-type bacteria in the stomach digesta of Lactobacillus-free mice. Despite the modulation of urea hydrolysis in the stomach, recycling of urea nitrogen in the murine host was not affected since the essential amino acid isoleucine, labeled with a stable isotope, was detected in the livers of both Lactobacillus-free and 100-23-colonized animals. Therefore, our experiments reveal a new and unexpected impact of Lactobacillus colonization on urea hydrolysis in the murine gut.}},
   = {10.1128/AEM.01876-14},
  source = {IRIS}
}