A Highly Sensitive Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilized on Cadmium Sulfide Quantum Dots/Chitosan Composite Modified Glassy Carbon Electrode

Nutthaya Butwong; Lin Zhou; Eric Moore; Supalax Srijaranai; John H.T. Luong; Jeremy D. Glennon

doi:10.1002/elan.201400353

A Highly Sensitive Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilized on Cadmium Sulfide Quantum Dots/Chitosan Composite Modified Glassy Carbon Electrode

Nutthaya Butwong
, Lin Zhou
, Eric Moore
, Supalax Srijaranai
, John H.T. Luong
, Jeremy D. Glennon

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

An L-cysteine capped cadmium sulfide-chitosan nanocomposite has been synthesized, characterized and used for surface modification of a glassy carbon electrode. With direct electron transfer, hemoglobin (Hb) adsorbed strongly on the nanocomposite and displayed excellent bioelectrocatalysis for H₂O₂. The biosensor was capable of reducing H₂O₂ at -0.35V, with a detection limit of 3.13nM, linearity in the range of 15nM to 10μM and a response time of less than 2s. The Michaelis-Menten constant (K_M) was 0.57nM, attesting high bioelectrocatalysis of immobilized Hb for H₂O₂. Reproducibility of the fabrication method was very satisfactory with a relative standard deviation of 5.3%. The biosensor lost only 6.5% of its original response after 7days when stored in a pH7.4 PBS at 4°C.

Original language	English
Pages (from-to)	2465-2473
Number of pages	9
Journal	Electroanalysis
Volume	26
Issue number	11
DOIs	https://doi.org/10.1002/elan.201400353
Publication status	Published - 1 Nov 2014

Keywords

Cadmium sulphide
Chitosan
Hemoglobin
Hydrogen peroxide
Quantum dots

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10.1002/elan.201400353

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title = "A Highly Sensitive Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilized on Cadmium Sulfide Quantum Dots/Chitosan Composite Modified Glassy Carbon Electrode",

abstract = "An L-cysteine capped cadmium sulfide-chitosan nanocomposite has been synthesized, characterized and used for surface modification of a glassy carbon electrode. With direct electron transfer, hemoglobin (Hb) adsorbed strongly on the nanocomposite and displayed excellent bioelectrocatalysis for H2O2. The biosensor was capable of reducing H2O2 at -0.35V, with a detection limit of 3.13nM, linearity in the range of 15nM to 10μM and a response time of less than 2s. The Michaelis-Menten constant (KM) was 0.57nM, attesting high bioelectrocatalysis of immobilized Hb for H2O2. Reproducibility of the fabrication method was very satisfactory with a relative standard deviation of 5.3\%. The biosensor lost only 6.5\% of its original response after 7days when stored in a pH7.4 PBS at 4°C.",

keywords = "Cadmium sulphide, Chitosan, Hemoglobin, Hydrogen peroxide, Quantum dots",

author = "Nutthaya Butwong and Lin Zhou and Eric Moore and Supalax Srijaranai and Luong, \{John H.T.\} and Glennon, \{Jeremy D.\}",

note = "Publisher Copyright: {\textcopyright} 2014 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.",

year = "2014",

month = nov,

day = "1",

doi = "10.1002/elan.201400353",

language = "English",

volume = "26",

pages = "2465--2473",

journal = "Electroanalysis",

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TY - JOUR

T1 - A Highly Sensitive Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilized on Cadmium Sulfide Quantum Dots/Chitosan Composite Modified Glassy Carbon Electrode

AU - Butwong, Nutthaya

AU - Zhou, Lin

AU - Moore, Eric

AU - Srijaranai, Supalax

AU - Luong, John H.T.

AU - Glennon, Jeremy D.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - An L-cysteine capped cadmium sulfide-chitosan nanocomposite has been synthesized, characterized and used for surface modification of a glassy carbon electrode. With direct electron transfer, hemoglobin (Hb) adsorbed strongly on the nanocomposite and displayed excellent bioelectrocatalysis for H2O2. The biosensor was capable of reducing H2O2 at -0.35V, with a detection limit of 3.13nM, linearity in the range of 15nM to 10μM and a response time of less than 2s. The Michaelis-Menten constant (KM) was 0.57nM, attesting high bioelectrocatalysis of immobilized Hb for H2O2. Reproducibility of the fabrication method was very satisfactory with a relative standard deviation of 5.3%. The biosensor lost only 6.5% of its original response after 7days when stored in a pH7.4 PBS at 4°C.

AB - An L-cysteine capped cadmium sulfide-chitosan nanocomposite has been synthesized, characterized and used for surface modification of a glassy carbon electrode. With direct electron transfer, hemoglobin (Hb) adsorbed strongly on the nanocomposite and displayed excellent bioelectrocatalysis for H2O2. The biosensor was capable of reducing H2O2 at -0.35V, with a detection limit of 3.13nM, linearity in the range of 15nM to 10μM and a response time of less than 2s. The Michaelis-Menten constant (KM) was 0.57nM, attesting high bioelectrocatalysis of immobilized Hb for H2O2. Reproducibility of the fabrication method was very satisfactory with a relative standard deviation of 5.3%. The biosensor lost only 6.5% of its original response after 7days when stored in a pH7.4 PBS at 4°C.

KW - Cadmium sulphide

KW - Chitosan

KW - Hemoglobin

KW - Hydrogen peroxide

KW - Quantum dots

UR - https://www.scopus.com/pages/publications/84910661467

U2 - 10.1002/elan.201400353

DO - 10.1002/elan.201400353

M3 - Article

AN - SCOPUS:84910661467

SN - 1040-0397

VL - 26

SP - 2465

EP - 2473

JO - Electroanalysis

JF - Electroanalysis

IS - 11

ER -

A Highly Sensitive Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilized on Cadmium Sulfide Quantum Dots/Chitosan Composite Modified Glassy Carbon Electrode

Abstract

Keywords

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