A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement

  • Somanna A. Kollimada
  • , Sambuddha Khan
  • , Sreenath Balakrishna
  • , Shilpa Raju
  • , Suma Matad
  • , G. K. Ananthasuresh

Research output: Chapter in Book/Report/Conference proceedingsChapterpeer-review

Abstract

(We describe the fabrication, characterization and operation of a microfabricated, overhanging micromachined SU-8 device that can stretch and compress individual cells. This compliant device is composed of folded beams with a single actuation point. Upon actuation with a glass micropipette attached to an XYZ positioner, it can apply varying deformation to multiple cells simultaneously. The applied force is computed using the measured displacement of the compliant device. Cell-attachment is achieved by simply adding a suspension of cells on the device. The stiffness of the device was designed such that cells can deform the beams they are attached to. Measuring this deformation in the absence of actuation allows for quantifying the forces exerted by the cells with a resolution of 0.18 μΝ.)

Original languageEnglish
Title of host publicationInternational Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings
EditorsSinan Haliyo, Sylvain Martel, Sergej Fatikow
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538603468
DOIs
Publication statusPublished - 3 Aug 2017
Externally publishedYes
Event1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Montreal, Canada
Duration: 17 Jul 201721 Jul 2017

Publication series

NameInternational Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings

Conference

Conference1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017
Country/TerritoryCanada
CityMontreal
Period17/07/1721/07/17

Keywords

  • Cell manipulation
  • Cellular force
  • Folded beam
  • Microfabrication
  • Su-8

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