A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement

Somanna A. Kollimada; Sambuddha Khan; Sreenath Balakrishna; Shilpa Raju; Suma Matad; G. K. Ananthasuresh

doi:10.1109/MARSS.2017.8001943

A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement

Somanna A. Kollimada
, Sambuddha Khan
, Sreenath Balakrishna
, Shilpa Raju
, Suma Matad
, G. K. Ananthasuresh

Indian Institute of Science Bangalore

Research output: Chapter in Book/Report/Conference proceedings › Conference proceeding › peer-review

Abstract

(We describe the fabrication, characterization and operation of a microfabricated, overhanging micromachined SU-8 device that can stretch and compress individual cells. This compliant device is composed of folded beams with a single actuation point. Upon actuation with a glass micropipette attached to an XYZ positioner, it can apply varying deformation to multiple cells simultaneously. The applied force is computed using the measured displacement of the compliant device. Cell-attachment is achieved by simply adding a suspension of cells on the device. The stiffness of the device was designed such that cells can deform the beams they are attached to. Measuring this deformation in the absence of actuation allows for quantifying the forces exerted by the cells with a resolution of 0.18 μΝ.)

Original language	English
Title of host publication	International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings
Editors	Sinan Haliyo, Sylvain Martel, Sergej Fatikow
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781538603468
DOIs	https://doi.org/10.1109/MARSS.2017.8001943
Publication status	Published - 3 Aug 2017
Externally published	Yes
Event	1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Montreal, Canada Duration: 17 Jul 2017 → 21 Jul 2017

Publication series

Name	International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings

Conference

Conference	1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017
Country/Territory	Canada
City	Montreal
Period	17/07/17 → 21/07/17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Keywords

Cell manipulation
Cellular force
Folded beam
Microfabrication
Su-8

Access to Document

10.1109/MARSS.2017.8001943

Cite this

Kollimada, S. A., Khan, S., Balakrishna, S., Raju, S., Matad, S., & Ananthasuresh, G. K. (2017). A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement. In S. Haliyo, S. Martel, & S. Fatikow (Eds.), International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings Article 8001943 (International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MARSS.2017.8001943

Kollimada, Somanna A. ; Khan, Sambuddha ; Balakrishna, Sreenath et al. / A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement. International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. editor / Sinan Haliyo ; Sylvain Martel ; Sergej Fatikow. Institute of Electrical and Electronics Engineers Inc., 2017. (International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings).

@inproceedings{4bac4ebdb45847e283a2554c40f6ff9d,

title = "A micro-mechanical compliant device for individual cell-stretching, compression, \& in-situ force-measurement",

abstract = "(We describe the fabrication, characterization and operation of a microfabricated, overhanging micromachined SU-8 device that can stretch and compress individual cells. This compliant device is composed of folded beams with a single actuation point. Upon actuation with a glass micropipette attached to an XYZ positioner, it can apply varying deformation to multiple cells simultaneously. The applied force is computed using the measured displacement of the compliant device. Cell-attachment is achieved by simply adding a suspension of cells on the device. The stiffness of the device was designed such that cells can deform the beams they are attached to. Measuring this deformation in the absence of actuation allows for quantifying the forces exerted by the cells with a resolution of 0.18 μΝ.)",

keywords = "Cell manipulation, Cellular force, Folded beam, Microfabrication, Su-8",

author = "Kollimada, \{Somanna A.\} and Sambuddha Khan and Sreenath Balakrishna and Shilpa Raju and Suma Matad and Ananthasuresh, \{G. K.\}",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 ; Conference date: 17-07-2017 Through 21-07-2017",

year = "2017",

month = aug,

day = "3",

doi = "10.1109/MARSS.2017.8001943",

language = "English",

series = "International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

editor = "Sinan Haliyo and Sylvain Martel and Sergej Fatikow",

booktitle = "International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings",

address = "United States",

}

Kollimada, SA, Khan, S, Balakrishna, S, Raju, S, Matad, S & Ananthasuresh, GK 2017, A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement. in S Haliyo, S Martel & S Fatikow (eds), International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings., 8001943, International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings, Institute of Electrical and Electronics Engineers Inc., 1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017, Montreal, Canada, 17/07/17. https://doi.org/10.1109/MARSS.2017.8001943

A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement. / Kollimada, Somanna A.; Khan, Sambuddha; Balakrishna, Sreenath et al.
International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. ed. / Sinan Haliyo; Sylvain Martel; Sergej Fatikow. Institute of Electrical and Electronics Engineers Inc., 2017. 8001943 (International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings).

Research output: Chapter in Book/Report/Conference proceedings › Conference proceeding › peer-review

TY - GEN

T1 - A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement

AU - Kollimada, Somanna A.

AU - Khan, Sambuddha

AU - Balakrishna, Sreenath

AU - Raju, Shilpa

AU - Matad, Suma

AU - Ananthasuresh, G. K.

PY - 2017/8/3

Y1 - 2017/8/3

N2 - (We describe the fabrication, characterization and operation of a microfabricated, overhanging micromachined SU-8 device that can stretch and compress individual cells. This compliant device is composed of folded beams with a single actuation point. Upon actuation with a glass micropipette attached to an XYZ positioner, it can apply varying deformation to multiple cells simultaneously. The applied force is computed using the measured displacement of the compliant device. Cell-attachment is achieved by simply adding a suspension of cells on the device. The stiffness of the device was designed such that cells can deform the beams they are attached to. Measuring this deformation in the absence of actuation allows for quantifying the forces exerted by the cells with a resolution of 0.18 μΝ.)

AB - (We describe the fabrication, characterization and operation of a microfabricated, overhanging micromachined SU-8 device that can stretch and compress individual cells. This compliant device is composed of folded beams with a single actuation point. Upon actuation with a glass micropipette attached to an XYZ positioner, it can apply varying deformation to multiple cells simultaneously. The applied force is computed using the measured displacement of the compliant device. Cell-attachment is achieved by simply adding a suspension of cells on the device. The stiffness of the device was designed such that cells can deform the beams they are attached to. Measuring this deformation in the absence of actuation allows for quantifying the forces exerted by the cells with a resolution of 0.18 μΝ.)

KW - Cell manipulation

KW - Cellular force

KW - Folded beam

KW - Microfabrication

KW - Su-8

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U2 - 10.1109/MARSS.2017.8001943

DO - 10.1109/MARSS.2017.8001943

M3 - Conference proceeding

AN - SCOPUS:85030256922

T3 - International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings

BT - International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings

A2 - Haliyo, Sinan

A2 - Martel, Sylvain

A2 - Fatikow, Sergej

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017

Y2 - 17 July 2017 through 21 July 2017

ER -

Kollimada SA, Khan S, Balakrishna S, Raju S, Matad S, Ananthasuresh GK. A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement. In Haliyo S, Martel S, Fatikow S, editors, International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2017. 8001943. (International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings). doi: 10.1109/MARSS.2017.8001943

A micro-mechanical compliant device for individual cell-stretching, compression, & in-situ force-measurement

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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