A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

Khaled A. Mahmoud; John H.T. Luong

doi:10.1080/00032711003653924

A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

Khaled A. Mahmoud
, John H.T. Luong

National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.

Original language	English
Pages (from-to)	1680-1687
Number of pages	8
Journal	Analytical Letters
Volume	43
Issue number	10-11
DOIs	https://doi.org/10.1080/00032711003653924
Publication status	Published - Jul 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Electrochemistry
Ferrocene
Gold nanoparticle
Hiv-1
Pepstatin
Protease
Single-walled carbon nanotube

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10.1080/00032711003653924

Cite this

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title = "A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode",

abstract = "A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.",

keywords = "Electrochemistry, Ferrocene, Gold nanoparticle, Hiv-1, Pepstatin, Protease, Single-walled carbon nanotube",

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year = "2010",

month = jul,

doi = "10.1080/00032711003653924",

language = "English",

volume = "43",

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T1 - A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

AU - Mahmoud, Khaled A.

AU - Luong, John H.T.

PY - 2010/7

Y1 - 2010/7

N2 - A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.

AB - A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.

KW - Electrochemistry

KW - Ferrocene

KW - Gold nanoparticle

KW - Hiv-1

KW - Pepstatin

KW - Protease

KW - Single-walled carbon nanotube

UR - https://www.scopus.com/pages/publications/77954856822

U2 - 10.1080/00032711003653924

DO - 10.1080/00032711003653924

M3 - Article

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SN - 0003-2719

VL - 43

SP - 1680

EP - 1687

JO - Analytical Letters

JF - Analytical Letters

IS - 10-11

ER -

A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

Abstract

UN SDGs

Keywords

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