A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches

Eoin Gunnigle; Jean Baptiste Ramond; Aline Frossard; Mary Seeley; Don Cowan

doi:10.1016/j.mimet.2014.06.004

A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches

Eoin Gunnigle
, Jean Baptiste Ramond
, Aline Frossard
, Mary Seeley
, Don Cowan

Research output: Contribution to journal › Article › peer-review

Abstract

A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.

Original language	English
Pages (from-to)	118-123
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	103
DOIs	https://doi.org/10.1016/j.mimet.2014.06.004
Publication status	Published - Aug 2014
Externally published	Yes

Keywords

Co-extraction
Metaproteomics
Nucleic acids
Protein
T-RFLP

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10.1016/j.mimet.2014.06.004

Cite this

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title = "A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches",

abstract = "A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.",

keywords = "Co-extraction, Metaproteomics, Nucleic acids, Protein, T-RFLP",

author = "Eoin Gunnigle and Ramond, \{Jean Baptiste\} and Aline Frossard and Mary Seeley and Don Cowan",

year = "2014",

month = aug,

doi = "10.1016/j.mimet.2014.06.004",

language = "English",

volume = "103",

pages = "118--123",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches

AU - Gunnigle, Eoin

AU - Ramond, Jean Baptiste

AU - Frossard, Aline

AU - Seeley, Mary

AU - Cowan, Don

PY - 2014/8

Y1 - 2014/8

N2 - A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.

AB - A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.

KW - Co-extraction

KW - Metaproteomics

KW - Nucleic acids

KW - Protein

KW - T-RFLP

UR - https://www.scopus.com/pages/publications/84904625116

U2 - 10.1016/j.mimet.2014.06.004

DO - 10.1016/j.mimet.2014.06.004

M3 - Article

C2 - 24929037

AN - SCOPUS:84904625116

SN - 0167-7012

VL - 103

SP - 118

EP - 123

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches

Abstract

Keywords

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