TY - CHAP
T1 - A smartphone-based colorimetric reader for human C-reactive protein immunoassay
AU - Venkatesh, A. G.
AU - van Oordt, Thomas
AU - Schneider, E. Marion
AU - Zengerle, Roland
AU - von Stetten, Felix
AU - Luong, John H.T.
AU - Vashist, Sandeep Kumar
N1 - Publisher Copyright:
© Springer Science+Business Media LLC 2017.
PY - 2017
Y1 - 2017
N2 - A smartphone-based colorimetric reader (SBCR), comprising a Samsung Galaxy SIII mini, a gadget (iPAD mini, iPAD4, or iPhone 5s) and a custom-made dark hood and base holder assembly, is used for human Creactive protein (CRP) immunoassay. A 96-well microtiter plate (MTP) is positioned on the gadget’s screensaver to provide white light-based bottom illumination only in the specific regions corresponding to the well’s bottom. The images captured by the smartphone’s back camera are analyzed by a novel image processing algorithm. Based on one-step kinetics-based human C-reactive protein immunoassay (IA), SBCR is evaluated and compared with a commercial MTP reader (MTPR). For analysis of CRP spiked in diluted human whole blood and plasma as well as CRP in clinical plasma samples, SBCR exhibits the same precision, dynamic range, detection limit, and sensitivity as MTPR for the developed IA (DIA). Considering its compactness, low cost, advanced features and a remarkable computing power, SBCR is an ideal point-of care (POC) colorimetric detection device for the next-generation of cost-effective POC testing (POCT).
AB - A smartphone-based colorimetric reader (SBCR), comprising a Samsung Galaxy SIII mini, a gadget (iPAD mini, iPAD4, or iPhone 5s) and a custom-made dark hood and base holder assembly, is used for human Creactive protein (CRP) immunoassay. A 96-well microtiter plate (MTP) is positioned on the gadget’s screensaver to provide white light-based bottom illumination only in the specific regions corresponding to the well’s bottom. The images captured by the smartphone’s back camera are analyzed by a novel image processing algorithm. Based on one-step kinetics-based human C-reactive protein immunoassay (IA), SBCR is evaluated and compared with a commercial MTP reader (MTPR). For analysis of CRP spiked in diluted human whole blood and plasma as well as CRP in clinical plasma samples, SBCR exhibits the same precision, dynamic range, detection limit, and sensitivity as MTPR for the developed IA (DIA). Considering its compactness, low cost, advanced features and a remarkable computing power, SBCR is an ideal point-of care (POC) colorimetric detection device for the next-generation of cost-effective POC testing (POCT).
KW - Graphene
KW - Human C-reactive protein
KW - Monitoring inflammation
KW - One-step kinetics
KW - Sandwich immunoassay
KW - Smartphone-based colorimetric reader
UR - https://www.scopus.com/pages/publications/85014992427
U2 - 10.1007/978-1-4939-6848-0_21
DO - 10.1007/978-1-4939-6848-0_21
M3 - Chapter
C2 - 28281266
AN - SCOPUS:85014992427
T3 - Methods in Molecular Biology
SP - 343
EP - 356
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -