Altered expression and secretion of colonic Interleukin-6 in a stress-sensitive animal model of brain-gut axis dysfunction

Dervla O'Malley; Timothy G. Dinan; John F. Cryan

doi:10.1016/j.jneuroim.2011.04.003

Altered expression and secretion of colonic Interleukin-6 in a stress-sensitive animal model of brain-gut axis dysfunction

Dervla O'Malley
, Timothy G. Dinan
, John F. Cryan

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

Interleukin-6 (IL-6) can activate gastrointestinal submucosal neurons, with associated implications for motility and secretory function. Patients with irritable bowel syndrome (IBS) have elevated levels of circulating IL-6. Colons from the Wistar Kyoto (WKY) rat model of IBS secrete more IL-6 (12.84. pg/ml) than control Sprague Dawley (SD) colons (5.55. pg/ml) and WKY secretions stimulated calcium responses in naïve submucosal neurons of greater amplitude. Recombinant IL-6 activated more submucosal neurons in WKY tissue preparations (p < 0.05). These data demonstrate that WKY colonic supernatants activate submucosal neurons using an IL-6-dependent mechanism, thereby providing a link between gastrointestinal dysfunction and alterations in IL-6 levels.

Original language	English
Pages (from-to)	48-55
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	235
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2011.04.003
Publication status	Published - Jun 2011

Keywords

Interleukin-6
Irritable bowel syndrome
Submucosal neurons
Wistar Kyoto rat

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10.1016/j.jneuroim.2011.04.003

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title = "Altered expression and secretion of colonic Interleukin-6 in a stress-sensitive animal model of brain-gut axis dysfunction",

abstract = "Interleukin-6 (IL-6) can activate gastrointestinal submucosal neurons, with associated implications for motility and secretory function. Patients with irritable bowel syndrome (IBS) have elevated levels of circulating IL-6. Colons from the Wistar Kyoto (WKY) rat model of IBS secrete more IL-6 (12.84. pg/ml) than control Sprague Dawley (SD) colons (5.55. pg/ml) and WKY secretions stimulated calcium responses in na{\"i}ve submucosal neurons of greater amplitude. Recombinant IL-6 activated more submucosal neurons in WKY tissue preparations (p < 0.05). These data demonstrate that WKY colonic supernatants activate submucosal neurons using an IL-6-dependent mechanism, thereby providing a link between gastrointestinal dysfunction and alterations in IL-6 levels.",

keywords = "Interleukin-6, Irritable bowel syndrome, Submucosal neurons, Wistar Kyoto rat",

author = "Dervla O'Malley and Dinan, \{Timothy G.\} and Cryan, \{John F.\}",

year = "2011",

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AU - Dinan, Timothy G.

AU - Cryan, John F.

PY - 2011/6

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AB - Interleukin-6 (IL-6) can activate gastrointestinal submucosal neurons, with associated implications for motility and secretory function. Patients with irritable bowel syndrome (IBS) have elevated levels of circulating IL-6. Colons from the Wistar Kyoto (WKY) rat model of IBS secrete more IL-6 (12.84. pg/ml) than control Sprague Dawley (SD) colons (5.55. pg/ml) and WKY secretions stimulated calcium responses in naïve submucosal neurons of greater amplitude. Recombinant IL-6 activated more submucosal neurons in WKY tissue preparations (p < 0.05). These data demonstrate that WKY colonic supernatants activate submucosal neurons using an IL-6-dependent mechanism, thereby providing a link between gastrointestinal dysfunction and alterations in IL-6 levels.

KW - Interleukin-6

KW - Irritable bowel syndrome

KW - Submucosal neurons

KW - Wistar Kyoto rat

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Altered expression and secretion of colonic Interleukin-6 in a stress-sensitive animal model of brain-gut axis dysfunction

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Keywords

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