An improved method for detection of Salmonella in foodstuffs using anti-Salmonella antibody

E. Prusak-Sochaczewski; J. H.T. Luong

doi:10.1016/0141-0229(89)90002-1

An improved method for detection of Salmonella in foodstuffs using anti-Salmonella antibody

E. Prusak-Sochaczewski
, J. H.T. Luong

National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid and reliable procedure was developed for detection of Salmonella spp. in foodstuffs. After pre-enrichment in nutrient broth for 6 h and an 18-h selective enrichment in Rappaport-Vassiliadis medium, cells were collected on a membrane filter. The presence of Salmonella cells was detected with enzyme-labeled polyclonal antibody to Salmonella common structural antigen (CSA-1). The detection method did not appear to be influenced by the presence of Escherichia coli, a commonly found interfering organisms in contaminated foodstuffs.

Original language	English
Pages (from-to)	645-649
Number of pages	5
Journal	Enzyme and Microbial Technology
Volume	11
Issue number	10
DOIs	https://doi.org/10.1016/0141-0229(89)90002-1
Publication status	Published - Oct 1989
Externally published	Yes

Keywords

antibody
antigen
detection
membrane
Salmonella

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10.1016/0141-0229(89)90002-1

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abstract = "A rapid and reliable procedure was developed for detection of Salmonella spp. in foodstuffs. After pre-enrichment in nutrient broth for 6 h and an 18-h selective enrichment in Rappaport-Vassiliadis medium, cells were collected on a membrane filter. The presence of Salmonella cells was detected with enzyme-labeled polyclonal antibody to Salmonella common structural antigen (CSA-1). The detection method did not appear to be influenced by the presence of Escherichia coli, a commonly found interfering organisms in contaminated foodstuffs.",

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AU - Luong, J. H.T.

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N2 - A rapid and reliable procedure was developed for detection of Salmonella spp. in foodstuffs. After pre-enrichment in nutrient broth for 6 h and an 18-h selective enrichment in Rappaport-Vassiliadis medium, cells were collected on a membrane filter. The presence of Salmonella cells was detected with enzyme-labeled polyclonal antibody to Salmonella common structural antigen (CSA-1). The detection method did not appear to be influenced by the presence of Escherichia coli, a commonly found interfering organisms in contaminated foodstuffs.

AB - A rapid and reliable procedure was developed for detection of Salmonella spp. in foodstuffs. After pre-enrichment in nutrient broth for 6 h and an 18-h selective enrichment in Rappaport-Vassiliadis medium, cells were collected on a membrane filter. The presence of Salmonella cells was detected with enzyme-labeled polyclonal antibody to Salmonella common structural antigen (CSA-1). The detection method did not appear to be influenced by the presence of Escherichia coli, a commonly found interfering organisms in contaminated foodstuffs.

KW - antibody

KW - antigen

KW - detection

KW - membrane

KW - Salmonella

UR - https://www.scopus.com/pages/publications/0024748338

U2 - 10.1016/0141-0229(89)90002-1

DO - 10.1016/0141-0229(89)90002-1

M3 - Article

AN - SCOPUS:0024748338

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VL - 11

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JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 10

ER -

An improved method for detection of Salmonella in foodstuffs using anti-Salmonella antibody

Abstract

Keywords

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