An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain

Gerard Clarke; Siobhain O'Mahony; Gwen Malone; Timothy G. Dinan

doi:10.1016/j.jneumeth.2006.09.006

An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain

Gerard Clarke
, Siobhain O'Mahony
, Gwen Malone
, Timothy G. Dinan

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

The amino acid neurotransmitters γ-aminobutyric acid (GABA) and glutamate have been implicated in mood disorders and high performance liquid chromatography (HPLC) is an important method in their quantitation. This paper describes a method which employs either electrochemical or fluorescent detection of the amino acid derivatives formed by a reaction with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ions. Elution is based on an isocratic protocol and is achieved in 20 min or less. The method is reproducible, shows excellent linearity and generates derivatives which are stable for up to 16 h. Successful application of the method to a maternal deprivation study has identified the hippocampus and the brainstem as possible sites of long-term alteration in this paradigm.

Original language	English
Pages (from-to)	223-230
Number of pages	8
Journal	Journal of Neuroscience Methods
Volume	160
Issue number	2
DOIs	https://doi.org/10.1016/j.jneumeth.2006.09.006
Publication status	Published - 15 Mar 2007

Keywords

GABA
Glutamate
High performance liquid chromatography
Isocratic protocol
Maternal deprivation
Naphthalene-2,3-dicarboxaldehyde

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10.1016/j.jneumeth.2006.09.006

Cite this

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title = "An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain",

abstract = "The amino acid neurotransmitters γ-aminobutyric acid (GABA) and glutamate have been implicated in mood disorders and high performance liquid chromatography (HPLC) is an important method in their quantitation. This paper describes a method which employs either electrochemical or fluorescent detection of the amino acid derivatives formed by a reaction with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ions. Elution is based on an isocratic protocol and is achieved in 20 min or less. The method is reproducible, shows excellent linearity and generates derivatives which are stable for up to 16 h. Successful application of the method to a maternal deprivation study has identified the hippocampus and the brainstem as possible sites of long-term alteration in this paradigm.",

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AU - Dinan, Timothy G.

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AB - The amino acid neurotransmitters γ-aminobutyric acid (GABA) and glutamate have been implicated in mood disorders and high performance liquid chromatography (HPLC) is an important method in their quantitation. This paper describes a method which employs either electrochemical or fluorescent detection of the amino acid derivatives formed by a reaction with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ions. Elution is based on an isocratic protocol and is achieved in 20 min or less. The method is reproducible, shows excellent linearity and generates derivatives which are stable for up to 16 h. Successful application of the method to a maternal deprivation study has identified the hippocampus and the brainstem as possible sites of long-term alteration in this paradigm.

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KW - Glutamate

KW - High performance liquid chromatography

KW - Isocratic protocol

KW - Maternal deprivation

KW - Naphthalene-2,3-dicarboxaldehyde

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An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain

Abstract

Keywords

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