Analysis of close proximity quenching of phosphorescent metalloporphyrin labels in oligonucleotide structures

M. Burke; P. J. O'Sullivan; G. V. Ponomarev; D. V. Yashunsky; D. B. Papkovsky

doi:10.1016/j.aca.2006.12.025

Analysis of close proximity quenching of phosphorescent metalloporphyrin labels in oligonucleotide structures

M. Burke
, P. J. O'Sullivan
, G. V. Ponomarev
, D. V. Yashunsky
, D. B. Papkovsky

School of Biochemistry and Cell Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Quenching of phosphorescent platinum(II) and palladium(II) coproporphyrin (MeCP) labelled oligonucleotides was investigated. Strong hybridization-specific quenching was observed in duplex DNA structures with a variety of quenchers and with two identical porphyrin labels when in close proximity. Classical resonance energy transfer mechanism was ruled out, since quenching did not correlate with spectral overlaps and lifetime changes were insignificant. Quenching of MeCP by the free quenchers in solution revealed that porphyrin-porphyrin quenching is predominantly static while other dyes quench dynamically. The results suggest that the quenching in DNA duplex proceeds via direct contact.

Original language	English
Pages (from-to)	139-146
Number of pages	8
Journal	Analytica Chimica Acta
Volume	585
Issue number	1
DOIs	https://doi.org/10.1016/j.aca.2006.12.025
Publication status	Published - 28 Feb 2007

Keywords

Close proximity quenching
Hybridization assays
Metalloporphyrin labels
Oligonucleotide probes
Phosphorescence

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10.1016/j.aca.2006.12.025

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title = "Analysis of close proximity quenching of phosphorescent metalloporphyrin labels in oligonucleotide structures",

abstract = "Quenching of phosphorescent platinum(II) and palladium(II) coproporphyrin (MeCP) labelled oligonucleotides was investigated. Strong hybridization-specific quenching was observed in duplex DNA structures with a variety of quenchers and with two identical porphyrin labels when in close proximity. Classical resonance energy transfer mechanism was ruled out, since quenching did not correlate with spectral overlaps and lifetime changes were insignificant. Quenching of MeCP by the free quenchers in solution revealed that porphyrin-porphyrin quenching is predominantly static while other dyes quench dynamically. The results suggest that the quenching in DNA duplex proceeds via direct contact.",

keywords = "Close proximity quenching, Hybridization assays, Metalloporphyrin labels, Oligonucleotide probes, Phosphorescence",

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T1 - Analysis of close proximity quenching of phosphorescent metalloporphyrin labels in oligonucleotide structures

AU - Burke, M.

AU - O'Sullivan, P. J.

AU - Ponomarev, G. V.

AU - Yashunsky, D. V.

AU - Papkovsky, D. B.

PY - 2007/2/28

Y1 - 2007/2/28

N2 - Quenching of phosphorescent platinum(II) and palladium(II) coproporphyrin (MeCP) labelled oligonucleotides was investigated. Strong hybridization-specific quenching was observed in duplex DNA structures with a variety of quenchers and with two identical porphyrin labels when in close proximity. Classical resonance energy transfer mechanism was ruled out, since quenching did not correlate with spectral overlaps and lifetime changes were insignificant. Quenching of MeCP by the free quenchers in solution revealed that porphyrin-porphyrin quenching is predominantly static while other dyes quench dynamically. The results suggest that the quenching in DNA duplex proceeds via direct contact.

AB - Quenching of phosphorescent platinum(II) and palladium(II) coproporphyrin (MeCP) labelled oligonucleotides was investigated. Strong hybridization-specific quenching was observed in duplex DNA structures with a variety of quenchers and with two identical porphyrin labels when in close proximity. Classical resonance energy transfer mechanism was ruled out, since quenching did not correlate with spectral overlaps and lifetime changes were insignificant. Quenching of MeCP by the free quenchers in solution revealed that porphyrin-porphyrin quenching is predominantly static while other dyes quench dynamically. The results suggest that the quenching in DNA duplex proceeds via direct contact.

KW - Close proximity quenching

KW - Hybridization assays

KW - Metalloporphyrin labels

KW - Oligonucleotide probes

KW - Phosphorescence

UR - https://www.scopus.com/pages/publications/33846643200

U2 - 10.1016/j.aca.2006.12.025

DO - 10.1016/j.aca.2006.12.025

M3 - Article

C2 - 17386658

AN - SCOPUS:33846643200

SN - 0003-2670

VL - 585

SP - 139

EP - 146

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

Analysis of close proximity quenching of phosphorescent metalloporphyrin labels in oligonucleotide structures

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