Analytical Techniques for Phytochemical Estimation in Fruit Juices

Analytical strategies for the detection of bioactive compounds, phytochemicals, contaminants, or any kind of adulteration in fruit juices must provide good protocols for sample preparation (clean-up, extraction, and derivatization), separation (conventional and instrumental chromatographic methods), and detection (i.e., ultraviolet-visible, diode array detectors, and flame ionization detectors). Many advanced technologies are available to date, and the application of the different methodologies for the analysis of a specific compound or a group of phytochemicals depends on the physicochemical properties of the analytes or their metabolites, the sample matrix, the extraction methods, and various spectroscopic and chromatographic techniques. This chapter focuses on the analytical protocols used in fruit juices and the multiple approaches developed to analyze a wide variety of compounds.