Antibiotic selection leads to inadvertent selection of nfxC-type phenotypic mutants in Pseudomonas aeruginosa

Marlies J. Mooij; Hazel F. O'Connor; Zhe Xian Tian; Yi Ping Wang; Claire Adams; John P. Morrissey; Fergal O'Gara

doi:10.1111/j.1758-2229.2010.00175.x

Antibiotic selection leads to inadvertent selection of nfxC-type phenotypic mutants in Pseudomonas aeruginosa

Marlies J. Mooij
, Hazel F. O'Connor
, Zhe Xian Tian
, Yi Ping Wang
, Claire Adams
, John P. Morrissey
, Fergal O'Gara

School of Microbiology

University College Cork
Peking University

Research output: Contribution to journal › Article › peer-review

Abstract

Spontaneous nfxC-type phenotypic mutants of Pseudomonas aeruginosa are characterized by chloramphenicol resistance and increased expression of the multidrug efflux pump, MexEF-OprN. These mutants frequently arise during the propagation of bacteria in laboratory conditions. This study shows that some of the discrepancies in transcriptomic/phenotypic profiles of two rsmA mutants, PAZH13 (rsmA mutant in strain PAO1) and PAKδrsmA, are due to the nfxC-type nature of PAZH13. This indicates that awareness of the possible occurrence of these spontaneous nfxC-type phenotypes is necessary. Screening of mexE levels could provide clarity when an nfxC-type phenotype is suspected.

Original language	English
Pages (from-to)	461-464
Number of pages	4
Journal	Environmental Microbiology Reports
Volume	2
Issue number	3
DOIs	https://doi.org/10.1111/j.1758-2229.2010.00175.x
Publication status	Published - Jun 2010

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10.1111/j.1758-2229.2010.00175.x

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title = "Antibiotic selection leads to inadvertent selection of nfxC-type phenotypic mutants in Pseudomonas aeruginosa",

abstract = "Spontaneous nfxC-type phenotypic mutants of Pseudomonas aeruginosa are characterized by chloramphenicol resistance and increased expression of the multidrug efflux pump, MexEF-OprN. These mutants frequently arise during the propagation of bacteria in laboratory conditions. This study shows that some of the discrepancies in transcriptomic/phenotypic profiles of two rsmA mutants, PAZH13 (rsmA mutant in strain PAO1) and PAKδrsmA, are due to the nfxC-type nature of PAZH13. This indicates that awareness of the possible occurrence of these spontaneous nfxC-type phenotypes is necessary. Screening of mexE levels could provide clarity when an nfxC-type phenotype is suspected.",

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year = "2010",

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language = "English",

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T1 - Antibiotic selection leads to inadvertent selection of nfxC-type phenotypic mutants in Pseudomonas aeruginosa

AU - Mooij, Marlies J.

AU - O'Connor, Hazel F.

AU - Tian, Zhe Xian

AU - Wang, Yi Ping

AU - Adams, Claire

AU - Morrissey, John P.

AU - O'Gara, Fergal

PY - 2010/6

Y1 - 2010/6

N2 - Spontaneous nfxC-type phenotypic mutants of Pseudomonas aeruginosa are characterized by chloramphenicol resistance and increased expression of the multidrug efflux pump, MexEF-OprN. These mutants frequently arise during the propagation of bacteria in laboratory conditions. This study shows that some of the discrepancies in transcriptomic/phenotypic profiles of two rsmA mutants, PAZH13 (rsmA mutant in strain PAO1) and PAKδrsmA, are due to the nfxC-type nature of PAZH13. This indicates that awareness of the possible occurrence of these spontaneous nfxC-type phenotypes is necessary. Screening of mexE levels could provide clarity when an nfxC-type phenotype is suspected.

AB - Spontaneous nfxC-type phenotypic mutants of Pseudomonas aeruginosa are characterized by chloramphenicol resistance and increased expression of the multidrug efflux pump, MexEF-OprN. These mutants frequently arise during the propagation of bacteria in laboratory conditions. This study shows that some of the discrepancies in transcriptomic/phenotypic profiles of two rsmA mutants, PAZH13 (rsmA mutant in strain PAO1) and PAKδrsmA, are due to the nfxC-type nature of PAZH13. This indicates that awareness of the possible occurrence of these spontaneous nfxC-type phenotypes is necessary. Screening of mexE levels could provide clarity when an nfxC-type phenotype is suspected.

UR - https://www.scopus.com/pages/publications/78649729735

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DO - 10.1111/j.1758-2229.2010.00175.x

M3 - Article

AN - SCOPUS:78649729735

SN - 1758-2229

VL - 2

SP - 461

EP - 464

JO - Environmental Microbiology Reports

JF - Environmental Microbiology Reports

IS - 3

ER -

Antibiotic selection leads to inadvertent selection of nfxC-type phenotypic mutants in Pseudomonas aeruginosa

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