Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysate from unicorn leatherjacket skin

Supatra Karnjanapratum; Yvonne C. O'Callaghan; Soottawat Benjakul; Nora O'Brien

doi:10.1002/jsfa.7504

Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysate from unicorn leatherjacket skin

Supatra Karnjanapratum
, Yvonne C. O'Callaghan
, Soottawat Benjakul
, Nora O'Brien

School of Food and Nutritional Sciences

Prince of Songkla University

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: The in vitro cellular bioactivities including, antioxidant, immunomodulatory and antiproliferative effects of a gelatin hydrolysate (GH) prepared from unicorn leatherjacket skin, using partially purified glycyl endopeptidase, were investigated in order to optimize the use of fish skin waste products as functional food ingredients. RESULTS: GH under the tested concentrations (750-1500 μg mL^-1) protected against H₂O₂-induced DNA damage in U937 cells. GH also protected against the H₂O₂-induced reduction in cellular antioxidant enzyme activities, superoxide dismutase and catalase, in HepG2 cells. GH demonstrated immunomodulatory potential by reducing pro-inflammatory cytokine (interleukin-6 (IL-6) and IL-1β) production and nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Cell proliferation in human colon cancer (Caco-2) cells was significantly reduced in a dose-dependent manner following incubation with GH. CONCLUSION: These results indicate that GH has several bioactivities which support its potential as a promising functional food ingredient with various health benefits.

Original language	English
Pages (from-to)	3220-3226
Number of pages	7
Journal	Journal of the Science of Food and Agriculture
Volume	96
Issue number	9
DOIs	https://doi.org/10.1002/jsfa.7504 https://doi.org/10.1002/jsfa.7504
Publication status	Published - 1 Jul 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Animals
Antioxidants/analysis
Caco-2 Cells/drug effects
Catalase/metabolism
Cell Proliferation/drug effects
Cysteine Endopeptidases/chemistry
Cytokines/metabolism
DNA Damage/drug effects
Dietary Supplements
Dose-Response Relationship, Drug
Enzyme Activation
Gelatin/chemistry
Hep G2 Cells
Humans
Lipopolysaccharides/pharmacology
Macrophages/drug effects
Mice
Nitric Oxide/biosynthesis
Perciformes/metabolism
Protein Hydrolysates/chemistry
RAW 264.7 Cells
Skin/chemistry
Superoxide Dismutase/metabolism
U937 Cells

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title = "Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysate from unicorn leatherjacket skin",

abstract = "BACKGROUND: The in vitro cellular bioactivities including, antioxidant, immunomodulatory and antiproliferative effects of a gelatin hydrolysate (GH) prepared from unicorn leatherjacket skin, using partially purified glycyl endopeptidase, were investigated in order to optimize the use of fish skin waste products as functional food ingredients. RESULTS: GH under the tested concentrations (750-1500 μg mL-1) protected against H2O2-induced DNA damage in U937 cells. GH also protected against the H2O2-induced reduction in cellular antioxidant enzyme activities, superoxide dismutase and catalase, in HepG2 cells. GH demonstrated immunomodulatory potential by reducing pro-inflammatory cytokine (interleukin-6 (IL-6) and IL-1β) production and nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Cell proliferation in human colon cancer (Caco-2) cells was significantly reduced in a dose-dependent manner following incubation with GH. CONCLUSION: These results indicate that GH has several bioactivities which support its potential as a promising functional food ingredient with various health benefits.",

keywords = "Animals, Antioxidants/analysis, Caco-2 Cells/drug effects, Catalase/metabolism, Cell Proliferation/drug effects, Cysteine Endopeptidases/chemistry, Cytokines/metabolism, DNA Damage/drug effects, Dietary Supplements, Dose-Response Relationship, Drug, Enzyme Activation, Gelatin/chemistry, Hep G2 Cells, Humans, Lipopolysaccharides/pharmacology, Macrophages/drug effects, Mice, Nitric Oxide/biosynthesis, Perciformes/metabolism, Protein Hydrolysates/chemistry, RAW 264.7 Cells, Skin/chemistry, Superoxide Dismutase/metabolism, U937 Cells",

author = "Supatra Karnjanapratum and O'Callaghan, \{Yvonne C.\} and Soottawat Benjakul and Nora O'Brien",

note = "Publisher Copyright: {\textcopyright} 2016 Society of Chemical Industry.",

year = "2016",

month = jul,

day = "1",

doi = "10.1002/jsfa.7504",

language = "English",

volume = "96",

pages = "3220--3226",

journal = "Journal of the Science of Food and Agriculture",

issn = "0022-5142",

publisher = "John Wiley and Sons Ltd",

number = "9",

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TY - JOUR

T1 - Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysate from unicorn leatherjacket skin

AU - Karnjanapratum, Supatra

AU - O'Callaghan, Yvonne C.

AU - Benjakul, Soottawat

AU - O'Brien, Nora

PY - 2016/7/1

Y1 - 2016/7/1

N2 - BACKGROUND: The in vitro cellular bioactivities including, antioxidant, immunomodulatory and antiproliferative effects of a gelatin hydrolysate (GH) prepared from unicorn leatherjacket skin, using partially purified glycyl endopeptidase, were investigated in order to optimize the use of fish skin waste products as functional food ingredients. RESULTS: GH under the tested concentrations (750-1500 μg mL-1) protected against H2O2-induced DNA damage in U937 cells. GH also protected against the H2O2-induced reduction in cellular antioxidant enzyme activities, superoxide dismutase and catalase, in HepG2 cells. GH demonstrated immunomodulatory potential by reducing pro-inflammatory cytokine (interleukin-6 (IL-6) and IL-1β) production and nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Cell proliferation in human colon cancer (Caco-2) cells was significantly reduced in a dose-dependent manner following incubation with GH. CONCLUSION: These results indicate that GH has several bioactivities which support its potential as a promising functional food ingredient with various health benefits.

AB - BACKGROUND: The in vitro cellular bioactivities including, antioxidant, immunomodulatory and antiproliferative effects of a gelatin hydrolysate (GH) prepared from unicorn leatherjacket skin, using partially purified glycyl endopeptidase, were investigated in order to optimize the use of fish skin waste products as functional food ingredients. RESULTS: GH under the tested concentrations (750-1500 μg mL-1) protected against H2O2-induced DNA damage in U937 cells. GH also protected against the H2O2-induced reduction in cellular antioxidant enzyme activities, superoxide dismutase and catalase, in HepG2 cells. GH demonstrated immunomodulatory potential by reducing pro-inflammatory cytokine (interleukin-6 (IL-6) and IL-1β) production and nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Cell proliferation in human colon cancer (Caco-2) cells was significantly reduced in a dose-dependent manner following incubation with GH. CONCLUSION: These results indicate that GH has several bioactivities which support its potential as a promising functional food ingredient with various health benefits.

KW - Animals

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KW - Caco-2 Cells/drug effects

KW - Catalase/metabolism

KW - Cell Proliferation/drug effects

KW - Cysteine Endopeptidases/chemistry

KW - Cytokines/metabolism

KW - DNA Damage/drug effects

KW - Dietary Supplements

KW - Dose-Response Relationship, Drug

KW - Enzyme Activation

KW - Gelatin/chemistry

KW - Hep G2 Cells

KW - Humans

KW - Lipopolysaccharides/pharmacology

KW - Macrophages/drug effects

KW - Mice

KW - Nitric Oxide/biosynthesis

KW - Perciformes/metabolism

KW - Protein Hydrolysates/chemistry

KW - RAW 264.7 Cells

KW - Skin/chemistry

KW - Superoxide Dismutase/metabolism

KW - U937 Cells

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M3 - Article

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SN - 0022-5142

VL - 96

SP - 3220

EP - 3226

JO - Journal of the Science of Food and Agriculture

JF - Journal of the Science of Food and Agriculture

IS - 9

ER -

Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysate from unicorn leatherjacket skin

Abstract

UN SDGs

Keywords

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