Bienzymatic assembly formed @ Pt nano sensing framework detecting acetylcholine in aqueous phase

N. Chauhan; S. Tiwari; T. Narayan; U. Jain

doi:10.1016/j.apsusc.2018.04.056

Bienzymatic assembly formed @ Pt nano sensing framework detecting acetylcholine in aqueous phase

N. Chauhan
, S. Tiwari
, T. Narayan
, U. Jain

Amity University, Noida

Research output: Contribution to journal › Article › peer-review

Abstract

Acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized on platinum nanoparticles (PtNPs) and metallic organic framework (MOF) modified Au electrode to construct an acetylcholine (ACh) biosensor. The morphology and structure of the nanoparticles modified working electrode were characterized by Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), Cyclic voltammetry (CVs) and Electrochemical Impedance Spectroscopy (EIS) techniques. The prepared biosensor exhibit a wide linear range of 0.01–500 μM for acetylthiocholine chloride (ATCl) and a low detection limit of 0.01 μM. No interferences were observed for other possible compounds/substances present in the serum samples. For detection of ACh in clinical (serum) samples the results of the nanoparticle based biosensor were well correlate with the results obtained from HPLC method. The half-life of the working electrode was 4 months, when stored at 4 °C.

Original language	English
Pages (from-to)	154-160
Number of pages	7
Journal	Applied Surface Science
Volume	474
DOIs	https://doi.org/10.1016/j.apsusc.2018.04.056
Publication status	Published - 30 Apr 2019
Externally published	Yes

Keywords

Acetylcholine
Acetylcholineesterase
Choline oxidase
Metallic organic framework
Platinum nanoparticles

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10.1016/j.apsusc.2018.04.056

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@article{88f1d0e230c04ca59943b5e2826fc32b,

title = "Bienzymatic assembly formed @ Pt nano sensing framework detecting acetylcholine in aqueous phase",

abstract = "Acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized on platinum nanoparticles (PtNPs) and metallic organic framework (MOF) modified Au electrode to construct an acetylcholine (ACh) biosensor. The morphology and structure of the nanoparticles modified working electrode were characterized by Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), Cyclic voltammetry (CVs) and Electrochemical Impedance Spectroscopy (EIS) techniques. The prepared biosensor exhibit a wide linear range of 0.01–500 μM for acetylthiocholine chloride (ATCl) and a low detection limit of 0.01 μM. No interferences were observed for other possible compounds/substances present in the serum samples. For detection of ACh in clinical (serum) samples the results of the nanoparticle based biosensor were well correlate with the results obtained from HPLC method. The half-life of the working electrode was 4 months, when stored at 4 °C.",

keywords = "Acetylcholine, Acetylcholineesterase, Choline oxidase, Metallic organic framework, Platinum nanoparticles",

author = "N. Chauhan and S. Tiwari and T. Narayan and U. Jain",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2019",

month = apr,

day = "30",

doi = "10.1016/j.apsusc.2018.04.056",

language = "English",

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journal = "Applied Surface Science",

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TY - JOUR

T1 - Bienzymatic assembly formed @ Pt nano sensing framework detecting acetylcholine in aqueous phase

AU - Chauhan, N.

AU - Tiwari, S.

AU - Narayan, T.

AU - Jain, U.

PY - 2019/4/30

Y1 - 2019/4/30

N2 - Acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized on platinum nanoparticles (PtNPs) and metallic organic framework (MOF) modified Au electrode to construct an acetylcholine (ACh) biosensor. The morphology and structure of the nanoparticles modified working electrode were characterized by Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), Cyclic voltammetry (CVs) and Electrochemical Impedance Spectroscopy (EIS) techniques. The prepared biosensor exhibit a wide linear range of 0.01–500 μM for acetylthiocholine chloride (ATCl) and a low detection limit of 0.01 μM. No interferences were observed for other possible compounds/substances present in the serum samples. For detection of ACh in clinical (serum) samples the results of the nanoparticle based biosensor were well correlate with the results obtained from HPLC method. The half-life of the working electrode was 4 months, when stored at 4 °C.

AB - Acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized on platinum nanoparticles (PtNPs) and metallic organic framework (MOF) modified Au electrode to construct an acetylcholine (ACh) biosensor. The morphology and structure of the nanoparticles modified working electrode were characterized by Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), Cyclic voltammetry (CVs) and Electrochemical Impedance Spectroscopy (EIS) techniques. The prepared biosensor exhibit a wide linear range of 0.01–500 μM for acetylthiocholine chloride (ATCl) and a low detection limit of 0.01 μM. No interferences were observed for other possible compounds/substances present in the serum samples. For detection of ACh in clinical (serum) samples the results of the nanoparticle based biosensor were well correlate with the results obtained from HPLC method. The half-life of the working electrode was 4 months, when stored at 4 °C.

KW - Acetylcholine

KW - Acetylcholineesterase

KW - Choline oxidase

KW - Metallic organic framework

KW - Platinum nanoparticles

UR - https://www.scopus.com/pages/publications/85045110023

U2 - 10.1016/j.apsusc.2018.04.056

DO - 10.1016/j.apsusc.2018.04.056

M3 - Article

AN - SCOPUS:85045110023

SN - 0169-4332

VL - 474

SP - 154

EP - 160

JO - Applied Surface Science

JF - Applied Surface Science

ER -

Bienzymatic assembly formed @ Pt nano sensing framework detecting acetylcholine in aqueous phase

Abstract

Keywords

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