Bifidobacterium longum modified recombinant HU protein as a vector for nonviral delivery of dna to HEK293 human cell culture

E. V. Khokhlova; B. A. Efimov; L. I. Kafarskaia; K. A. Pavlov; A. N. Shkoporov

doi:10.1007/s10517-011-1424-7

Bifidobacterium longum modified recombinant HU protein as a vector for nonviral delivery of dna to HEK293 human cell culture

E. V. Khokhlova
, B. A. Efimov
, L. I. Kafarskaia
, K. A. Pavlov
, A. N. Shkoporov

Pirogov Russian National Research Medical University

Research output: Contribution to journal › Article › peer-review

Abstract

Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding histone-like protein HU were obtained. The resultant recombinant proteins bind to plasmid DNA in vitro and provide intracellular delivery and expression of the reporter genetic construction with GFP gene in cultured HEK293 human cells.

Original language	English
Pages (from-to)	717-721
Number of pages	5
Journal	Bulletin of Experimental Biology and Medicine
Volume	151
Issue number	6
DOIs	https://doi.org/10.1007/s10517-011-1424-7
Publication status	Published - Oct 2011
Externally published	Yes

Keywords

bifi dobacteria
cell-penetrating peptides
histonelike proteins
nonviral delivery of DNA
PTD domains

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10.1007/s10517-011-1424-7

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title = "Bifidobacterium longum modified recombinant HU protein as a vector for nonviral delivery of dna to HEK293 human cell culture",

abstract = "Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding histone-like protein HU were obtained. The resultant recombinant proteins bind to plasmid DNA in vitro and provide intracellular delivery and expression of the reporter genetic construction with GFP gene in cultured HEK293 human cells.",

keywords = "bifi dobacteria, cell-penetrating peptides, histonelike proteins, nonviral delivery of DNA, PTD domains",

author = "Khokhlova, \{E. V.\} and Efimov, \{B. A.\} and Kafarskaia, \{L. I.\} and Pavlov, \{K. A.\} and Shkoporov, \{A. N.\}",

year = "2011",

month = oct,

doi = "10.1007/s10517-011-1424-7",

language = "English",

volume = "151",

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T1 - Bifidobacterium longum modified recombinant HU protein as a vector for nonviral delivery of dna to HEK293 human cell culture

AU - Khokhlova, E. V.

AU - Efimov, B. A.

AU - Kafarskaia, L. I.

AU - Pavlov, K. A.

AU - Shkoporov, A. N.

PY - 2011/10

Y1 - 2011/10

N2 - Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding histone-like protein HU were obtained. The resultant recombinant proteins bind to plasmid DNA in vitro and provide intracellular delivery and expression of the reporter genetic construction with GFP gene in cultured HEK293 human cells.

AB - Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding histone-like protein HU were obtained. The resultant recombinant proteins bind to plasmid DNA in vitro and provide intracellular delivery and expression of the reporter genetic construction with GFP gene in cultured HEK293 human cells.

KW - bifi dobacteria

KW - cell-penetrating peptides

KW - histonelike proteins

KW - nonviral delivery of DNA

KW - PTD domains

UR - https://www.scopus.com/pages/publications/80755128164

U2 - 10.1007/s10517-011-1424-7

DO - 10.1007/s10517-011-1424-7

M3 - Article

C2 - 22485216

AN - SCOPUS:80755128164

SN - 0007-4888

VL - 151

SP - 717

EP - 721

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 6

ER -

Bifidobacterium longum modified recombinant HU protein as a vector for nonviral delivery of dna to HEK293 human cell culture

Abstract

Keywords

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