@inbook{9e3fa95b6a804c8a87b00c0642a447d4,
title = "Bioconjugated gold nanoparticles enhance siRNA delivery in prostate cancer cells",
abstract = "Here we describe a simple way to create a gold nanoparticle (AuNP)-based non-viral delivery system to deliver siRNA into prostate cancer cells. Therefore, positively charged polyethylenimine (PEI)-capped AuNPs were synthesized in water and further conjugated with the targeting ligand (folic acid) for folate receptors (AuNPs-PEI-FA). The AuNPs-PEI-FA could effectively complex small interfering RNA (siRNA) through electrostatic interaction. Flow cytometry displayed that AuNPs-PEI-FA could specifically deliver siRNA into LNCaP cells, a prostate cancer cell line overexpressing prostate-specific membrane antigen (PSMA) that exhibits a hydrolase enzymatic activity with a folate substrate. In contrast, internalization of siRNA into PC-3 cells, a prostate cancer cell line not expressing PSMA or folate receptors, was not achieved using AuNPs-PEI-FA.siRNA. Following endolysosomal escape, the AuNPs-PEI-FA-.siRNA formulation resulted in significant endogenous gene silencing when compared to the nontargeted formulation, suggesting the potential of AuNPs-PEI-FA for targeted delivery of therapeutic siRNAs in the treatment of prostate cancer.",
keywords = "Gene therapy, Gold nanoparticles, Non-viral siRNA delivery, Prostate cancer, Receptor-mediated internalization, Targeting ligands",
author = "Kamil Rahme and Jianfeng Guo and Holmes, \{Justin D.\}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9220-1\_21",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "291--301",
booktitle = "Methods in Molecular Biology",
}
