TY - JOUR
T1 - Biosensors on the basis of luminescent oxygen sensor
T2 - The use of microporous light-scattering support materials
AU - Papkovsky, Dmitri B.
AU - Ovchinnikov, Alexandr N.
AU - Ogurtsov, Vladimir I.
AU - Ponomarev, Gelii V.
AU - Korpela, Timo
PY - 1998/8/31
Y1 - 1998/8/31
N2 - New sensor technology and materials for luminescence-based oxygen sensors are described, which utilise microporous light-scattering support in sensor fabrication. Such materials display a number of new features and advantages over conventional oxygen sensor membranes, such as, significantly increased levels of luminescent signals obtainable from the sensor, better mechanical, physical and optical properties and a high capacity for loading biomaterial. The new oxygen sensor technology enables simple and robust procedures of sensor fabrication, as well as saving of the sensing materials, bringing down sensor photobleaching, and improvement in reliability of measurements and signal resolution. Biosensors developed on the basis of the above luminescent oxygen-sensitive materials used as chemical transducers, are described. Direct immobilisation of oxidase enzymes of the oxygen sensor membranes allows simple assays of metabolites. In particular, new batch-type enzyme biosensor for glucose has been investigated, using a new heterogeneous assay format. The latter allows simple, fast and highly reproducible analysis of important metabolites.
AB - New sensor technology and materials for luminescence-based oxygen sensors are described, which utilise microporous light-scattering support in sensor fabrication. Such materials display a number of new features and advantages over conventional oxygen sensor membranes, such as, significantly increased levels of luminescent signals obtainable from the sensor, better mechanical, physical and optical properties and a high capacity for loading biomaterial. The new oxygen sensor technology enables simple and robust procedures of sensor fabrication, as well as saving of the sensing materials, bringing down sensor photobleaching, and improvement in reliability of measurements and signal resolution. Biosensors developed on the basis of the above luminescent oxygen-sensitive materials used as chemical transducers, are described. Direct immobilisation of oxidase enzymes of the oxygen sensor membranes allows simple assays of metabolites. In particular, new batch-type enzyme biosensor for glucose has been investigated, using a new heterogeneous assay format. The latter allows simple, fast and highly reproducible analysis of important metabolites.
KW - Luminescent
KW - Oxygen sensor
KW - Sensor membrane
UR - https://www.scopus.com/pages/publications/0032130927
U2 - 10.1016/S0925-4005(98)00171-3
DO - 10.1016/S0925-4005(98)00171-3
M3 - Article
AN - SCOPUS:0032130927
SN - 0925-4005
VL - 51
SP - 137
EP - 145
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 1-3
ER -