Boron doped diamond biosensor for detection of Escherichia coli

Ehsan Majid; Keith B. Male; John H.T. Luong

doi:10.1021/jf8013958

Boron doped diamond biosensor for detection of Escherichia coli

Ehsan Majid
, Keith B. Male
, John H.T. Luong

School of Chemistry

National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

Abstract

o-Nitrophenol, released from o-nitrophenyl-β-D-galactopyranose as catalyzed by β-galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl-β-D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50°C. A biphasic calibration plot was observed: 4 × 10⁴ to 2 × 10⁵ cells/mL and 2 × 10⁵ to 6 × 10⁶ cells/mL for the first and second region, respectively. The detection limit was 4 × 10⁴ cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by ∼40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.

Original language	English
Pages (from-to)	7691-7695
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	56
Issue number	17
DOIs	https://doi.org/10.1021/jf8013958
Publication status	Published - 10 Sep 2008

Keywords

β-galactosidase
Boron doped diamond
E. coli
Electrochemical detection
o-nitrophenyl-β-D-galactopyranose

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10.1021/jf8013958

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@article{4d8654f0074a4cb89d4cf1552b549d1d,

title = "Boron doped diamond biosensor for detection of Escherichia coli",

abstract = "o-Nitrophenol, released from o-nitrophenyl-β-D-galactopyranose as catalyzed by β-galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl-β-D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50°C. A biphasic calibration plot was observed: 4 × 104 to 2 × 105 cells/mL and 2 × 105 to 6 × 106 cells/mL for the first and second region, respectively. The detection limit was 4 × 104 cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by ∼40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.",

keywords = "β-galactosidase, Boron doped diamond, E. coli, Electrochemical detection, o-nitrophenyl-β-D-galactopyranose",

author = "Ehsan Majid and Male, \{Keith B.\} and Luong, \{John H.T.\}",

year = "2008",

month = sep,

day = "10",

doi = "10.1021/jf8013958",

language = "English",

volume = "56",

pages = "7691--7695",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "17",

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TY - JOUR

T1 - Boron doped diamond biosensor for detection of Escherichia coli

AU - Majid, Ehsan

AU - Male, Keith B.

AU - Luong, John H.T.

PY - 2008/9/10

Y1 - 2008/9/10

N2 - o-Nitrophenol, released from o-nitrophenyl-β-D-galactopyranose as catalyzed by β-galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl-β-D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50°C. A biphasic calibration plot was observed: 4 × 104 to 2 × 105 cells/mL and 2 × 105 to 6 × 106 cells/mL for the first and second region, respectively. The detection limit was 4 × 104 cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by ∼40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.

AB - o-Nitrophenol, released from o-nitrophenyl-β-D-galactopyranose as catalyzed by β-galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl-β-D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50°C. A biphasic calibration plot was observed: 4 × 104 to 2 × 105 cells/mL and 2 × 105 to 6 × 106 cells/mL for the first and second region, respectively. The detection limit was 4 × 104 cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by ∼40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.

KW - β-galactosidase

KW - Boron doped diamond

KW - E. coli

KW - Electrochemical detection

KW - o-nitrophenyl-β-D-galactopyranose

UR - https://www.scopus.com/pages/publications/52649125334

U2 - 10.1021/jf8013958

DO - 10.1021/jf8013958

M3 - Article

C2 - 18698792

AN - SCOPUS:52649125334

SN - 0021-8561

VL - 56

SP - 7691

EP - 7695

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 17

ER -

Boron doped diamond biosensor for detection of Escherichia coli

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Keywords

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