Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells

K. Rahme; J. Guo; S. Biswas; C. M. O'Driscoll; J. D. Holmes

Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells

K. Rahme
, J. Guo
, S. Biswas
, C. M. O'Driscoll
, J. D. Holmes

School of Chemistry

Research output: Chapter in Book/Report/Conference proceedings › Conference proceeding › peer-review

Abstract

Herein we describe a simple method for the synthesis of different sizes of polyethylenimine-capped gold nanoparticles (AuNPs-PEI) in water and assess their potential to deliver siRNA to cancer cells. AuNP-PEI with diameters ranging between 25-150 ran have been synthesised in aqueous solutions using PEI (25 KD and 2KD) as capping ligands, and hydroxylamine-O-sulfonic acid or ascorbic acid as reducing agents. Different parameters were found to affect the final size of nanoparticles core (i.e. gold salt concentrations, PEI molecular weight/concentrations, and temperature). The obtained AuNP-PEIs were characterized using UV-visible spectroscopy, Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS). The ability of AuNP-PEIs to complex siRNA was analysed by gel electrophoresis, while the potential application of AuNP-PEIs in siRNA delivery was investigated using PC-3 prostate cancer cells.

Original language	English
Title of host publication	Advanced Materials - TechConnect Briefs 2017
Editors	Matthew Laudon, Fiona Case, Bart Romanowicz, Fiona Case
Publisher	TechConnect
Pages	159-162
Number of pages	4
ISBN (Electronic)	9780997511789
Publication status	Published - 2017
Event	11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference - Washington, United States Duration: 14 May 2017 → 17 May 2017

Publication series

Name	Advanced Materials - TechConnect Briefs 2017
Volume	1

Conference

Conference	11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference
Country/Territory	United States
City	Washington
Period	14/05/17 → 17/05/17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer
Gold nanoparticles
Polymers
SiRNA delivery
Stabilization

Cite this

@inproceedings{659ca21a292641f09e918e1bf73eca02,

title = "Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells",

abstract = "Herein we describe a simple method for the synthesis of different sizes of polyethylenimine-capped gold nanoparticles (AuNPs-PEI) in water and assess their potential to deliver siRNA to cancer cells. AuNP-PEI with diameters ranging between 25-150 ran have been synthesised in aqueous solutions using PEI (25 KD and 2KD) as capping ligands, and hydroxylamine-O-sulfonic acid or ascorbic acid as reducing agents. Different parameters were found to affect the final size of nanoparticles core (i.e. gold salt concentrations, PEI molecular weight/concentrations, and temperature). The obtained AuNP-PEIs were characterized using UV-visible spectroscopy, Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS). The ability of AuNP-PEIs to complex siRNA was analysed by gel electrophoresis, while the potential application of AuNP-PEIs in siRNA delivery was investigated using PC-3 prostate cancer cells.",

keywords = "Cancer, Gold nanoparticles, Polymers, SiRNA delivery, Stabilization",

author = "K. Rahme and J. Guo and S. Biswas and O'Driscoll, \{C. M.\} and Holmes, \{J. D.\}",

year = "2017",

language = "English",

series = "Advanced Materials - TechConnect Briefs 2017",

publisher = "TechConnect",

pages = "159--162",

editor = "Matthew Laudon and Fiona Case and Bart Romanowicz and Fiona Case",

booktitle = "Advanced Materials - TechConnect Briefs 2017",

note = "11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference ; Conference date: 14-05-2017 Through 17-05-2017",

}

Rahme, K, Guo, J, Biswas, S, O'Driscoll, CM & Holmes, JD 2017, Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells. in M Laudon, F Case, B Romanowicz & F Case (eds), Advanced Materials - TechConnect Briefs 2017. Advanced Materials - TechConnect Briefs 2017, vol. 1, TechConnect, pp. 159-162, 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference, Washington, United States, 14/05/17.

Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells. / Rahme, K.; Guo, J.; Biswas, S. et al.
Advanced Materials - TechConnect Briefs 2017. ed. / Matthew Laudon; Fiona Case; Bart Romanowicz; Fiona Case. TechConnect, 2017. p. 159-162 (Advanced Materials - TechConnect Briefs 2017; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedings › Conference proceeding › peer-review

TY - GEN

T1 - Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells

AU - Rahme, K.

AU - Guo, J.

AU - Biswas, S.

AU - O'Driscoll, C. M.

AU - Holmes, J. D.

PY - 2017

Y1 - 2017

N2 - Herein we describe a simple method for the synthesis of different sizes of polyethylenimine-capped gold nanoparticles (AuNPs-PEI) in water and assess their potential to deliver siRNA to cancer cells. AuNP-PEI with diameters ranging between 25-150 ran have been synthesised in aqueous solutions using PEI (25 KD and 2KD) as capping ligands, and hydroxylamine-O-sulfonic acid or ascorbic acid as reducing agents. Different parameters were found to affect the final size of nanoparticles core (i.e. gold salt concentrations, PEI molecular weight/concentrations, and temperature). The obtained AuNP-PEIs were characterized using UV-visible spectroscopy, Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS). The ability of AuNP-PEIs to complex siRNA was analysed by gel electrophoresis, while the potential application of AuNP-PEIs in siRNA delivery was investigated using PC-3 prostate cancer cells.

AB - Herein we describe a simple method for the synthesis of different sizes of polyethylenimine-capped gold nanoparticles (AuNPs-PEI) in water and assess their potential to deliver siRNA to cancer cells. AuNP-PEI with diameters ranging between 25-150 ran have been synthesised in aqueous solutions using PEI (25 KD and 2KD) as capping ligands, and hydroxylamine-O-sulfonic acid or ascorbic acid as reducing agents. Different parameters were found to affect the final size of nanoparticles core (i.e. gold salt concentrations, PEI molecular weight/concentrations, and temperature). The obtained AuNP-PEIs were characterized using UV-visible spectroscopy, Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS). The ability of AuNP-PEIs to complex siRNA was analysed by gel electrophoresis, while the potential application of AuNP-PEIs in siRNA delivery was investigated using PC-3 prostate cancer cells.

KW - Cancer

KW - Gold nanoparticles

KW - Polymers

KW - SiRNA delivery

KW - Stabilization

UR - https://www.scopus.com/pages/publications/85029436925

M3 - Conference proceeding

AN - SCOPUS:85029436925

T3 - Advanced Materials - TechConnect Briefs 2017

SP - 159

EP - 162

BT - Advanced Materials - TechConnect Briefs 2017

A2 - Laudon, Matthew

A2 - Case, Fiona

A2 - Romanowicz, Bart

A2 - Case, Fiona

PB - TechConnect

T2 - 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference

Y2 - 14 May 2017 through 17 May 2017

ER -

Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells

Abstract

Publication series

Conference

UN SDGs

Keywords

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