Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures

Marcel A.K. Jansen; Hilbert Booij; Jan H.N. Schel; Sacco C. de Vries

doi:10.1007/BF00232184

Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures

Marcel A.K. Jansen
, Hilbert Booij
, Jan H.N. Schel
, Sacco C. de Vries

Wageningen University & Research

Research output: Contribution to journal › Article › peer-review

Abstract

An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10^-3 M were transferred to hormone-free medium containing 10^-2 M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10^-4 M calcium were transferred to hormone-free medium with 10^-3 M calcium. At calcium concentrations between 6·10^-3 and 10^-2 M globular stage somatic embryos were found in cultures supplemented with 2·10^-6 M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations.

Original language	English
Pages (from-to)	221-223
Number of pages	3
Journal	Plant Cell Reports
Volume	9
Issue number	4
DOIs	https://doi.org/10.1007/BF00232184
Publication status	Published - Aug 1990
Externally published	Yes

Keywords

Calcium
Daucus carota
Somatic embryogenesis

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10.1007/BF00232184

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title = "Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures",

abstract = "An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10-3 M were transferred to hormone-free medium containing 10-2 M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10-4 M calcium were transferred to hormone-free medium with 10-3 M calcium. At calcium concentrations between 6·10-3 and 10-2 M globular stage somatic embryos were found in cultures supplemented with 2·10-6 M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations.",

keywords = "Calcium, Daucus carota, Somatic embryogenesis",

author = "Jansen, \{Marcel A.K.\} and Hilbert Booij and Schel, \{Jan H.N.\} and \{de Vries\}, \{Sacco C.\}",

year = "1990",

month = aug,

doi = "10.1007/BF00232184",

language = "English",

volume = "9",

pages = "221--223",

journal = "Plant Cell Reports",

issn = "0721-7714",

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}

TY - JOUR

T1 - Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures

AU - Jansen, Marcel A.K.

AU - Booij, Hilbert

AU - Schel, Jan H.N.

AU - de Vries, Sacco C.

PY - 1990/8

Y1 - 1990/8

N2 - An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10-3 M were transferred to hormone-free medium containing 10-2 M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10-4 M calcium were transferred to hormone-free medium with 10-3 M calcium. At calcium concentrations between 6·10-3 and 10-2 M globular stage somatic embryos were found in cultures supplemented with 2·10-6 M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations.

AB - An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10-3 M were transferred to hormone-free medium containing 10-2 M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10-4 M calcium were transferred to hormone-free medium with 10-3 M calcium. At calcium concentrations between 6·10-3 and 10-2 M globular stage somatic embryos were found in cultures supplemented with 2·10-6 M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations.

KW - Calcium

KW - Daucus carota

KW - Somatic embryogenesis

UR - https://www.scopus.com/pages/publications/34249959455

U2 - 10.1007/BF00232184

DO - 10.1007/BF00232184

M3 - Article

AN - SCOPUS:34249959455

SN - 0721-7714

VL - 9

SP - 221

EP - 223

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 4

ER -

Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures

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Keywords

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