Characterization of a virulent Lactobacillus sake phage PWH2

Renata G.K. Leuschner; Elke K. Arendt; Walter P. Hammes

doi:10.1007/BF00205063

Characterization of a virulent Lactobacillus sake phage PWH2

Renata G.K. Leuschner
, Elke K. Arendt
, Walter P. Hammes

University of Hohenheim

Research output: Contribution to journal › Article › peer-review

Abstract

A virulent bacteriophage PWH2 was isolated from fermented sausage. Out of 14 strains of Lactobacillus sake and 5 strains of L. curvatus tested as potential hosts, only L. sake Ls2 was sensitive. The plaques had a diameter of 0.5-1 mm. One-step growth kinetics of bacteriophage PWH2 revealed the following characteristics: a latent period of 1.5 h, a rise period of 1 h and a burst size of 110 (⁺_- 10) phages per cell. From electron micrographs it was deduced that bacteriophage PWH2 has an icosahedral head, a long non-contractile tail and a complex base plate. The genome is linear and 35 kbp in length. The structural proteins consist of three major and two minor proteins. After an infection of L. sake Ls2, isolates were obtained that were resistant to PWH2. By treatment with mitomycin C, isolate R4a was found to be lysogenic. DNA/DNA hybridisation proved homology of phage PWH2 with the chromosomal DNA of strain R4a.

Original language	English
Pages (from-to)	617-621
Number of pages	5
Journal	Applied Microbiology and Biotechnology
Volume	39
Issue number	4-5
DOIs	https://doi.org/10.1007/BF00205063
Publication status	Published - Jul 1993
Externally published	Yes

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title = "Characterization of a virulent Lactobacillus sake phage PWH2",

abstract = "A virulent bacteriophage PWH2 was isolated from fermented sausage. Out of 14 strains of Lactobacillus sake and 5 strains of L. curvatus tested as potential hosts, only L. sake Ls2 was sensitive. The plaques had a diameter of 0.5-1 mm. One-step growth kinetics of bacteriophage PWH2 revealed the following characteristics: a latent period of 1.5 h, a rise period of 1 h and a burst size of 110 (+- 10) phages per cell. From electron micrographs it was deduced that bacteriophage PWH2 has an icosahedral head, a long non-contractile tail and a complex base plate. The genome is linear and 35 kbp in length. The structural proteins consist of three major and two minor proteins. After an infection of L. sake Ls2, isolates were obtained that were resistant to PWH2. By treatment with mitomycin C, isolate R4a was found to be lysogenic. DNA/DNA hybridisation proved homology of phage PWH2 with the chromosomal DNA of strain R4a.",

author = "Leuschner, \{Renata G.K.\} and Arendt, \{Elke K.\} and Hammes, \{Walter P.\}",

year = "1993",

month = jul,

doi = "10.1007/BF00205063",

language = "English",

volume = "39",

pages = "617--621",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

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number = "4-5",

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TY - JOUR

T1 - Characterization of a virulent Lactobacillus sake phage PWH2

AU - Leuschner, Renata G.K.

AU - Arendt, Elke K.

AU - Hammes, Walter P.

PY - 1993/7

Y1 - 1993/7

N2 - A virulent bacteriophage PWH2 was isolated from fermented sausage. Out of 14 strains of Lactobacillus sake and 5 strains of L. curvatus tested as potential hosts, only L. sake Ls2 was sensitive. The plaques had a diameter of 0.5-1 mm. One-step growth kinetics of bacteriophage PWH2 revealed the following characteristics: a latent period of 1.5 h, a rise period of 1 h and a burst size of 110 (+- 10) phages per cell. From electron micrographs it was deduced that bacteriophage PWH2 has an icosahedral head, a long non-contractile tail and a complex base plate. The genome is linear and 35 kbp in length. The structural proteins consist of three major and two minor proteins. After an infection of L. sake Ls2, isolates were obtained that were resistant to PWH2. By treatment with mitomycin C, isolate R4a was found to be lysogenic. DNA/DNA hybridisation proved homology of phage PWH2 with the chromosomal DNA of strain R4a.

AB - A virulent bacteriophage PWH2 was isolated from fermented sausage. Out of 14 strains of Lactobacillus sake and 5 strains of L. curvatus tested as potential hosts, only L. sake Ls2 was sensitive. The plaques had a diameter of 0.5-1 mm. One-step growth kinetics of bacteriophage PWH2 revealed the following characteristics: a latent period of 1.5 h, a rise period of 1 h and a burst size of 110 (+- 10) phages per cell. From electron micrographs it was deduced that bacteriophage PWH2 has an icosahedral head, a long non-contractile tail and a complex base plate. The genome is linear and 35 kbp in length. The structural proteins consist of three major and two minor proteins. After an infection of L. sake Ls2, isolates were obtained that were resistant to PWH2. By treatment with mitomycin C, isolate R4a was found to be lysogenic. DNA/DNA hybridisation proved homology of phage PWH2 with the chromosomal DNA of strain R4a.

UR - https://www.scopus.com/pages/publications/0027291574

U2 - 10.1007/BF00205063

DO - 10.1007/BF00205063

M3 - Article

AN - SCOPUS:0027291574

SN - 0175-7598

VL - 39

SP - 617

EP - 621

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 4-5

ER -

Characterization of a virulent Lactobacillus sake phage PWH2

Abstract

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