Characterization of virus-like particles associated with the human faecal and caecal microbiota

Lesley Hoyles; Anne L. McCartney; Horst Neve; Glenn R. Gibson; Jeremy D. Sanderson; Knut J. Heller; Douwe van Sinderen

doi:10.1016/j.resmic.2014.10.006

Characterization of virus-like particles associated with the human faecal and caecal microbiota

Lesley Hoyles
, Anne L. McCartney
, Horst Neve
, Glenn R. Gibson
, Jeremy D. Sanderson
, Knut J. Heller
, Douwe van Sinderen

Research output: Contribution to journal › Article › peer-review

Abstract

This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.

Original language	English
Pages (from-to)	803-812
Number of pages	10
Journal	Research in Microbiology
Volume	165
Issue number	10
DOIs	https://doi.org/10.1016/j.resmic.2014.10.006
Publication status	Published - 1 Dec 2014

Keywords

Bacteriophages
Caecum
Faeces
Gastrointestinal tract
Microbial ecology
Transmission electron microscopy

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10.1016/j.resmic.2014.10.006

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title = "Characterization of virus-like particles associated with the human faecal and caecal microbiota",

abstract = "This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.",

keywords = "Bacteriophages, Caecum, Faeces, Gastrointestinal tract, Microbial ecology, Transmission electron microscopy",

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AU - Hoyles, Lesley

AU - McCartney, Anne L.

AU - Neve, Horst

AU - Gibson, Glenn R.

AU - Sanderson, Jeremy D.

AU - Heller, Knut J.

AU - van Sinderen, Douwe

PY - 2014/12/1

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AB - This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.

KW - Bacteriophages

KW - Caecum

KW - Faeces

KW - Gastrointestinal tract

KW - Microbial ecology

KW - Transmission electron microscopy

UR - https://www.scopus.com/pages/publications/84918533502

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EP - 812

JO - Research in Microbiology

JF - Research in Microbiology

IS - 10

ER -

Characterization of virus-like particles associated with the human faecal and caecal microbiota

Abstract

Keywords

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