Cloning and expression of rat GFAP cDNA in Escherichia coli

V. P. Chekhonin; K. A. Pavlov; A. N. Shkoporov; B. A. Yefimov; O. I. Gurina; T. B. Dmitrieva

doi:10.1007/s10517-007-0019-9

Cloning and expression of rat GFAP cDNA in Escherichia coli

V. P. Chekhonin
, K. A. Pavlov
, A. N. Shkoporov
, B. A. Yefimov
, O. I. Gurina
, T. B. Dmitrieva

Research output: Contribution to journal › Article › peer-review

Abstract

A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.

Original language	English
Pages (from-to)	68-71
Number of pages	4
Journal	Bulletin of Experimental Biology and Medicine
Volume	143
Issue number	1
DOIs	https://doi.org/10.1007/s10517-007-0019-9
Publication status	Published - Jan 2007
Externally published	Yes

Keywords

Central nervous system
Escherichia coli
GFAP recombinant protein
Glial fibrillary acidic protein (GFAP)

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10.1007/s10517-007-0019-9

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keywords = "Central nervous system, Escherichia coli, GFAP recombinant protein, Glial fibrillary acidic protein (GFAP)",

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AU - Chekhonin, V. P.

AU - Pavlov, K. A.

AU - Shkoporov, A. N.

AU - Yefimov, B. A.

AU - Gurina, O. I.

AU - Dmitrieva, T. B.

PY - 2007/1

Y1 - 2007/1

N2 - A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.

AB - A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.

KW - Central nervous system

KW - Escherichia coli

KW - GFAP recombinant protein

KW - Glial fibrillary acidic protein (GFAP)

UR - https://www.scopus.com/pages/publications/34249040626

U2 - 10.1007/s10517-007-0019-9

DO - 10.1007/s10517-007-0019-9

M3 - Article

C2 - 18019016

AN - SCOPUS:34249040626

SN - 0007-4888

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SP - 68

EP - 71

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 1

ER -

Cloning and expression of rat GFAP cDNA in Escherichia coli

Abstract

Keywords

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