Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363

Mary O'Connell-Motherway; Gerald F. Fitzgerald; Douwe Van Sinderen

doi:10.1128/aem.63.6.2454-2459.1997

Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363

Mary O'Connell-Motherway
, Gerald F. Fitzgerald
, Douwe Van Sinderen

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

Eight recombinant plasmids harboring chromosomal fragments of Lactococcus lactis MG1363 were shown to phenotypically suppress a histidine protein kinase (HPK) deficiency in either of two different E. coli strains. Sequence analysis of the plasmid inserts revealed five different complete or partial open reading frames (ORFs) specifying proteins with high similarity to HPKs. One of the plasmids also harbored an additional ORF, unrelated to HPKs, with suppressing activity.

Original language	English
Pages (from-to)	2454-2459
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	63
Issue number	6
DOIs	https://doi.org/10.1128/aem.63.6.2454-2459.1997
Publication status	Published - Jun 1997

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10.1128/aem.63.6.2454-2459.1997

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title = "Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363",

abstract = "Eight recombinant plasmids harboring chromosomal fragments of Lactococcus lactis MG1363 were shown to phenotypically suppress a histidine protein kinase (HPK) deficiency in either of two different E. coli strains. Sequence analysis of the plasmid inserts revealed five different complete or partial open reading frames (ORFs) specifying proteins with high similarity to HPKs. One of the plasmids also harbored an additional ORF, unrelated to HPKs, with suppressing activity.",

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AB - Eight recombinant plasmids harboring chromosomal fragments of Lactococcus lactis MG1363 were shown to phenotypically suppress a histidine protein kinase (HPK) deficiency in either of two different E. coli strains. Sequence analysis of the plasmid inserts revealed five different complete or partial open reading frames (ORFs) specifying proteins with high similarity to HPKs. One of the plasmids also harbored an additional ORF, unrelated to HPKs, with suppressing activity.

UR - https://www.scopus.com/pages/publications/0030984908

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DO - 10.1128/aem.63.6.2454-2459.1997

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Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363

Abstract

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