Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species

Luca Settanni; Sara Valmorri; Douwe Van Sinderen; Giovanna Suzzi; Antonello Paparella; Aldo Corsetti

doi:10.1128/AEM.72.5.3793-3796.2006

Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species

Luca Settanni
, Sara Valmorri
, Douwe Van Sinderen
, Giovanna Suzzi
, Antonello Paparella
, Aldo Corsetti

University of Teramo

Research output: Contribution to journal › Article › peer-review

Abstract

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

Original language	English
Pages (from-to)	3793-3796
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	5
DOIs	https://doi.org/10.1128/AEM.72.5.3793-3796.2006
Publication status	Published - May 2006

Access to Document

10.1128/AEM.72.5.3793-3796.2006

Cite this

@article{525a494b025c4cc1a697204b4bf68868,

title = "Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species",

abstract = "A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.",

author = "Luca Settanni and Sara Valmorri and \{Van Sinderen\}, Douwe and Giovanna Suzzi and Antonello Paparella and Aldo Corsetti",

year = "2006",

month = may,

doi = "10.1128/AEM.72.5.3793-3796.2006",

language = "English",

volume = "72",

pages = "3793--3796",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "5",

}

TY - JOUR

T1 - Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species

AU - Settanni, Luca

AU - Valmorri, Sara

AU - Van Sinderen, Douwe

AU - Suzzi, Giovanna

AU - Paparella, Antonello

AU - Corsetti, Aldo

PY - 2006/5

Y1 - 2006/5

N2 - A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

AB - A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

UR - https://www.scopus.com/pages/publications/33646581332

U2 - 10.1128/AEM.72.5.3793-3796.2006

DO - 10.1128/AEM.72.5.3793-3796.2006

M3 - Article

C2 - 16672538

AN - SCOPUS:33646581332

SN - 0099-2240

VL - 72

SP - 3793

EP - 3796

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 5

ER -

Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species

Abstract

Access to Document

Other files and links

Fingerprint

Cite this