Comparison of the three optical platforms for measurement of cellular respiration

Alina V. Kondrashina; Vladimir I. Ogurtsov; Dmitri B. Papkovsky

doi:10.1016/j.ab.2014.09.001

Comparison of the three optical platforms for measurement of cellular respiration

Alina V. Kondrashina
, Vladimir I. Ogurtsov
, Dmitri B. Papkovsky

School of Biochemistry and Cell Biology

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O₂ probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.

Original language	English
Pages (from-to)	1-3
Number of pages	3
Journal	Analytical Biochemistry
Volume	468
DOIs	https://doi.org/10.1016/j.ab.2014.09.001
Publication status	Published - 1 Jan 2015

Keywords

Cell oxygenation
Cell respiration
Intracellular oxygen
Oxygen consumption rate

Access to Document

10.1016/j.ab.2014.09.001

Cite this

@article{3b99896302174705a680713ba5eddcc1,

title = "Comparison of the three optical platforms for measurement of cellular respiration",

abstract = "We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.",

keywords = "Cell oxygenation, Cell respiration, Intracellular oxygen, Oxygen consumption rate",

author = "Kondrashina, \{Alina V.\} and Ogurtsov, \{Vladimir I.\} and Papkovsky, \{Dmitri B.\}",

year = "2015",

month = jan,

day = "1",

doi = "10.1016/j.ab.2014.09.001",

language = "English",

volume = "468",

pages = "1--3",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Comparison of the three optical platforms for measurement of cellular respiration

AU - Kondrashina, Alina V.

AU - Ogurtsov, Vladimir I.

AU - Papkovsky, Dmitri B.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.

AB - We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.

KW - Cell oxygenation

KW - Cell respiration

KW - Intracellular oxygen

KW - Oxygen consumption rate

UR - https://www.scopus.com/pages/publications/84908455597

U2 - 10.1016/j.ab.2014.09.001

DO - 10.1016/j.ab.2014.09.001

M3 - Article

C2 - 25233002

AN - SCOPUS:84908455597

SN - 0003-2697

VL - 468

SP - 1

EP - 3

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Comparison of the three optical platforms for measurement of cellular respiration

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this