Conjugative transfer of the transposon Tn919 to lactic acid bacteria

C. Hill; C. Daly; G. F. Fitzgerald

doi:10.1111/j.1574-6968.1985.tb00996.x

Conjugative transfer of the transposon Tn919 to lactic acid bacteria

C. Hill
, C. Daly
, G. F. Fitzgerald

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

The streptococcal transposon Tn919 was transferred from Streptococcus faecalis GF590 to selected Group N Streptococcus strains and to one strain each of Lactobacillus plantarum and Leuconostoc cremoris, using the filter mating method. An S. lactis MG1363 Rif^r Tc^r transconjugant also acted as a donor, but was less efficient than GF590. Frequencies of transfer varied between 4.0 × 10^-8 and 5.29 × 10^-5 per recipient. Further analysis of S. lactis MG1363 Sm^r Tc^r transconjugants showed that insertion of Tn919 into the chromosome was site-specific.

Original language	English
Pages (from-to)	115-119
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	30
Issue number	1-2
DOIs	https://doi.org/10.1111/j.1574-6968.1985.tb00996.x
Publication status	Published - 1985

Keywords

chromosomal insertion
Leuconostoc
S. lactis MG1363
site specificity

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10.1111/j.1574-6968.1985.tb00996.x

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title = "Conjugative transfer of the transposon Tn919 to lactic acid bacteria",

abstract = "The streptococcal transposon Tn919 was transferred from Streptococcus faecalis GF590 to selected Group N Streptococcus strains and to one strain each of Lactobacillus plantarum and Leuconostoc cremoris, using the filter mating method. An S. lactis MG1363 Rifr Tcr transconjugant also acted as a donor, but was less efficient than GF590. Frequencies of transfer varied between 4.0 × 10-8 and 5.29 × 10-5 per recipient. Further analysis of S. lactis MG1363 Smr Tcr transconjugants showed that insertion of Tn919 into the chromosome was site-specific.",

keywords = "chromosomal insertion, Leuconostoc, S. lactis MG1363, site specificity",

author = "C. Hill and C. Daly and Fitzgerald, \{G. F.\}",

year = "1985",

doi = "10.1111/j.1574-6968.1985.tb00996.x",

language = "English",

volume = "30",

pages = "115--119",

journal = "FEMS Microbiology Letters",

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TY - JOUR

T1 - Conjugative transfer of the transposon Tn919 to lactic acid bacteria

AU - Hill, C.

AU - Daly, C.

AU - Fitzgerald, G. F.

PY - 1985

Y1 - 1985

N2 - The streptococcal transposon Tn919 was transferred from Streptococcus faecalis GF590 to selected Group N Streptococcus strains and to one strain each of Lactobacillus plantarum and Leuconostoc cremoris, using the filter mating method. An S. lactis MG1363 Rifr Tcr transconjugant also acted as a donor, but was less efficient than GF590. Frequencies of transfer varied between 4.0 × 10-8 and 5.29 × 10-5 per recipient. Further analysis of S. lactis MG1363 Smr Tcr transconjugants showed that insertion of Tn919 into the chromosome was site-specific.

AB - The streptococcal transposon Tn919 was transferred from Streptococcus faecalis GF590 to selected Group N Streptococcus strains and to one strain each of Lactobacillus plantarum and Leuconostoc cremoris, using the filter mating method. An S. lactis MG1363 Rifr Tcr transconjugant also acted as a donor, but was less efficient than GF590. Frequencies of transfer varied between 4.0 × 10-8 and 5.29 × 10-5 per recipient. Further analysis of S. lactis MG1363 Smr Tcr transconjugants showed that insertion of Tn919 into the chromosome was site-specific.

KW - chromosomal insertion

KW - Leuconostoc

KW - S. lactis MG1363

KW - site specificity

UR - https://www.scopus.com/pages/publications/0022348416

U2 - 10.1111/j.1574-6968.1985.tb00996.x

DO - 10.1111/j.1574-6968.1985.tb00996.x

M3 - Article

AN - SCOPUS:0022348416

SN - 0378-1097

VL - 30

SP - 115

EP - 119

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1-2

ER -

Conjugative transfer of the transposon Tn919 to lactic acid bacteria

Abstract

Keywords

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