Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria

Christian U. Riedel; Pat G. Casey; Heidi Mulcahy; Fergal O'Gara; Cormac G.M. Gahan; Colin Hill

doi:10.1128/AEM.01394-07

Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria

Christian U. Riedel
, Pat G. Casey
, Heidi Mulcahy
, Fergal O'Gara
, Cormac G.M. Gahan
, Colin Hill

Research output: Contribution to journal › Article › peer-review

Abstract

A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.

Original language	English
Pages (from-to)	7092-7095
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	73
Issue number	21
DOIs	https://doi.org/10.1128/AEM.01394-07
Publication status	Published - Nov 2007

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title = "Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria",

abstract = "A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.",

author = "Riedel, \{Christian U.\} and Casey, \{Pat G.\} and Heidi Mulcahy and Fergal O'Gara and Gahan, \{Cormac G.M.\} and Colin Hill",

year = "2007",

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language = "English",

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AU - Riedel, Christian U.

AU - Casey, Pat G.

AU - Mulcahy, Heidi

AU - O'Gara, Fergal

AU - Gahan, Cormac G.M.

AU - Hill, Colin

PY - 2007/11

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N2 - A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.

AB - A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.

UR - https://www.scopus.com/pages/publications/35948956713

U2 - 10.1128/AEM.01394-07

DO - 10.1128/AEM.01394-07

M3 - Article

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AN - SCOPUS:35948956713

SN - 0099-2240

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EP - 7095

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 21

ER -

Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria

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