Design and validation of CRISPR/cas9 systems for targeted gene modification in induced pluripotent stem cells

Ciaran M. Lee; Haibao Zhu; Timothy H. Davis; Harshahardhan Deshmukh; Gang Bao

doi:10.1007/978-1-4939-6472-7_1

Design and validation of CRISPR/cas9 systems for targeted gene modification in induced pluripotent stem cells

Ciaran M. Lee
, Haibao Zhu
, Timothy H. Davis
, Harshahardhan Deshmukh
, Gang Bao

Rice University

Research output: Contribution to journal › Article › peer-review

Abstract

The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.

Original language	English
Pages (from-to)	3-21
Number of pages	19
Journal	Methods in Molecular Biology
Volume	1498
DOIs	https://doi.org/10.1007/978-1-4939-6472-7_1
Publication status	Published - 2017
Externally published	Yes

Keywords

CRISPR
Genome editing
Targeted gene knockout

Access to Document

10.1007/978-1-4939-6472-7_1

Cite this

@article{164937a575004a3e83ecb3e389ed790a,

title = "Design and validation of CRISPR/cas9 systems for targeted gene modification in induced pluripotent stem cells",

abstract = "The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.",

keywords = "CRISPR, Genome editing, Targeted gene knockout",

author = "Lee, \{Ciaran M.\} and Haibao Zhu and Davis, \{Timothy H.\} and Harshahardhan Deshmukh and Gang Bao",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",

year = "2017",

doi = "10.1007/978-1-4939-6472-7\_1",

language = "English",

volume = "1498",

pages = "3--21",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - Design and validation of CRISPR/cas9 systems for targeted gene modification in induced pluripotent stem cells

AU - Lee, Ciaran M.

AU - Zhu, Haibao

AU - Davis, Timothy H.

AU - Deshmukh, Harshahardhan

AU - Bao, Gang

N1 - Publisher Copyright: © Springer Science+Business Media New York 2017.

PY - 2017

Y1 - 2017

N2 - The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.

AB - The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.

KW - CRISPR

KW - Genome editing

KW - Targeted gene knockout

UR - https://www.scopus.com/pages/publications/84990985657

U2 - 10.1007/978-1-4939-6472-7_1

DO - 10.1007/978-1-4939-6472-7_1

M3 - Article

C2 - 27709565

AN - SCOPUS:84990985657

SN - 1064-3745

VL - 1498

SP - 3

EP - 21

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Design and validation of CRISPR/cas9 systems for targeted gene modification in induced pluripotent stem cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this