Detection of Fusobacterium species in human feces using genus-specific PCR primers and denaturing gradient gel electrophoresis

Jens Walter; Dirk Margosch; Walter P. Hammes; Christian Hertel

doi:10.1080/089106002320644294

Detection of Fusobacterium species in human feces using genus-specific PCR primers and denaturing gradient gel electrophoresis

Jens Walter
, Dirk Margosch
, Walter P. Hammes
, Christian Hertel

University of Hohenheim

Research output: Contribution to journal › Article › peer-review

Abstract

Denaturing gradient gel electrophoresis of 16S rDNA fragments generated with a genus-specific PCR system was used to detect Fusobacterium species in human feces, with the exception of F. prausnitzii. Out of 30 fecal samples, four contained fusobacteria at numbers close to the detection-limit of approximately 10⁵ cells per g, thus questioning a substantial role for these species in the intestinal flora.

Original language	English
Pages (from-to)	129-132
Number of pages	4
Journal	Microbial Ecology in Health and Disease
Volume	14
Issue number	3
DOIs	https://doi.org/10.1080/089106002320644294
Publication status	Published - Sep 2002
Externally published	Yes

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10.1080/089106002320644294

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title = "Detection of Fusobacterium species in human feces using genus-specific PCR primers and denaturing gradient gel electrophoresis",

abstract = "Denaturing gradient gel electrophoresis of 16S rDNA fragments generated with a genus-specific PCR system was used to detect Fusobacterium species in human feces, with the exception of F. prausnitzii. Out of 30 fecal samples, four contained fusobacteria at numbers close to the detection-limit of approximately 105 cells per g, thus questioning a substantial role for these species in the intestinal flora.",

author = "Jens Walter and Dirk Margosch and Hammes, \{Walter P.\} and Christian Hertel",

year = "2002",

month = sep,

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AU - Walter, Jens

AU - Margosch, Dirk

AU - Hammes, Walter P.

AU - Hertel, Christian

PY - 2002/9

Y1 - 2002/9

N2 - Denaturing gradient gel electrophoresis of 16S rDNA fragments generated with a genus-specific PCR system was used to detect Fusobacterium species in human feces, with the exception of F. prausnitzii. Out of 30 fecal samples, four contained fusobacteria at numbers close to the detection-limit of approximately 105 cells per g, thus questioning a substantial role for these species in the intestinal flora.

AB - Denaturing gradient gel electrophoresis of 16S rDNA fragments generated with a genus-specific PCR system was used to detect Fusobacterium species in human feces, with the exception of F. prausnitzii. Out of 30 fecal samples, four contained fusobacteria at numbers close to the detection-limit of approximately 105 cells per g, thus questioning a substantial role for these species in the intestinal flora.

UR - https://www.scopus.com/pages/publications/0036759049

U2 - 10.1080/089106002320644294

DO - 10.1080/089106002320644294

M3 - Article

AN - SCOPUS:0036759049

SN - 0891-060X

VL - 14

SP - 129

EP - 132

JO - Microbial Ecology in Health and Disease

JF - Microbial Ecology in Health and Disease

IS - 3

ER -

Detection of Fusobacterium species in human feces using genus-specific PCR primers and denaturing gradient gel electrophoresis

Abstract

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