Determination of haemolymph protein in Manila clam (Ruditapes philippinarum) by Lowry's method

Qiushi Xie; Gavin M. Burnell

doi:10.1016/0305-0491(94)90193-7

Determination of haemolymph protein in Manila clam (Ruditapes philippinarum) by Lowry's method

Qiushi Xie
, Gavin M. Burnell

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

Haemolymph protein of Manila clam (Ruditapes philippinarum) was assayed with Lowry's method. Comparison was made between different approaches of coping with the interference by Mg²⁺ and Ca²⁺. The method of Bensadoun and Weinstein and double-precipitation of protein with 6-7.5% TCA were both effective. Incorporating interfering substances in the reagent blank and protein standards also helped to correct the interference. The absorbances of 0.3 M and 1.0 M NaOH-pretreated haemolymph samples were 1.7 ± 0.12 and 2.12 ± 0.20 (mean ± SD, N = 20) times higher than that without NaOH pretreatment. Pretreatment of protein standards (BSA) with NaOH did not have obvious effects on their absorbance.

Original language	English
Pages (from-to)	609-612
Number of pages	4
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	107
Issue number	4
DOIs	https://doi.org/10.1016/0305-0491(94)90193-7
Publication status	Published - Apr 1994

Keywords

Haemolymph protein
Lowry's method
Ruditapes philippinarum

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10.1016/0305-0491(94)90193-7

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title = "Determination of haemolymph protein in Manila clam (Ruditapes philippinarum) by Lowry's method",

abstract = "Haemolymph protein of Manila clam (Ruditapes philippinarum) was assayed with Lowry's method. Comparison was made between different approaches of coping with the interference by Mg2+ and Ca2+. The method of Bensadoun and Weinstein and double-precipitation of protein with 6-7.5\% TCA were both effective. Incorporating interfering substances in the reagent blank and protein standards also helped to correct the interference. The absorbances of 0.3 M and 1.0 M NaOH-pretreated haemolymph samples were 1.7 ± 0.12 and 2.12 ± 0.20 (mean ± SD, N = 20) times higher than that without NaOH pretreatment. Pretreatment of protein standards (BSA) with NaOH did not have obvious effects on their absorbance.",

keywords = "Haemolymph protein, Lowry's method, Ruditapes philippinarum",

author = "Qiushi Xie and Burnell, \{Gavin M.\}",

year = "1994",

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doi = "10.1016/0305-0491(94)90193-7",

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T1 - Determination of haemolymph protein in Manila clam (Ruditapes philippinarum) by Lowry's method

AU - Xie, Qiushi

AU - Burnell, Gavin M.

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AB - Haemolymph protein of Manila clam (Ruditapes philippinarum) was assayed with Lowry's method. Comparison was made between different approaches of coping with the interference by Mg2+ and Ca2+. The method of Bensadoun and Weinstein and double-precipitation of protein with 6-7.5% TCA were both effective. Incorporating interfering substances in the reagent blank and protein standards also helped to correct the interference. The absorbances of 0.3 M and 1.0 M NaOH-pretreated haemolymph samples were 1.7 ± 0.12 and 2.12 ± 0.20 (mean ± SD, N = 20) times higher than that without NaOH pretreatment. Pretreatment of protein standards (BSA) with NaOH did not have obvious effects on their absorbance.

KW - Haemolymph protein

KW - Lowry's method

KW - Ruditapes philippinarum

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DO - 10.1016/0305-0491(94)90193-7

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SN - 1096-4959

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EP - 612

JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

IS - 4

ER -

Determination of haemolymph protein in Manila clam (Ruditapes philippinarum) by Lowry's method

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