Development of a lab-on-chip electrochemical immunosensor for detection of Polycyclic Aromatic Hydrocarbons (PAH) in environmental water

  • Shifa Felemban
  • , Patricia Vazquez
  • , Jan Dehnert
  • , Vadim Goridko
  • , Maria Tijero
  • , Eric Moore

Research output: Chapter in Book/Report/Conference proceedingsChapterpeer-review

Abstract

The work described in this manuscript focuses on how the integration of immunoassay techniques in combination with electrochemical detection can provide a portable and very accurate solution for detection of water pollutants that are detrimental for human health. In particular, we focus our work on the quantification of polycyclic aromatic hydrocarbons (PAHs) in polluted water. Our integrative approach facilitates a real-time detection of this family of organic compounds, by reducing the time of analysis to less than one hour. Additionally, the use of a lab-on-a-chip platform delivers a portable solution that could be used in situ. Optimization of a displacement assay that investigates the presence and concentration of Benzo[a]pyrene in water, allows with the miniaturization of the standard ELISA format into a highly accurate system that provides fast results. The limits of detection obtained are comparable to those of available state-of-the art tools, and achieve the values set by European Drinking Water Directive, 0.10ng/l, as the limit for PAHs in drinking water.

Original languageEnglish
Title of host publicationSmart Sensors, Actuators, and MEMS VIII
EditorsMika Prunnila, Luis Fonseca, Erwin Peiner
PublisherSPIE
ISBN (Electronic)9781510609938
DOIs
Publication statusPublished - 2017
EventSmart Sensors, Actuators, and MEMS VIII Conference 2017 - Barcelona, Spain
Duration: 8 May 201710 May 2017

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10246
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceSmart Sensors, Actuators, and MEMS VIII Conference 2017
Country/TerritorySpain
CityBarcelona
Period8/05/1710/05/17

Keywords

  • Benzo(a)pyrene
  • environmental monitoring
  • lab-on-a-chip electrochemical immunosensor
  • Polycyclic aromatic hydrocarbons (PAHs)

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