TY - JOUR
T1 - Disrupted mucosal vascular barrier in eosinophilic esophagitis
AU - Lenti, Marco Vincenzo
AU - Rossi, Carlo Maria
AU - Cannizzaro, Renato
AU - Santacroce, Giovanni
AU - Lizier, Michela
AU - Fornasa, Giulia
AU - Merli, Stefania
AU - Pasini, Alessandra
AU - Savarino, Edoardo Vincenzo
AU - Spessotto, Paola
AU - Di Stefano, Michele
AU - Mauro, Aurelio
AU - Vanoli, Alessandro
AU - Zingone, Fabiana
AU - Anderloni, Andrea
AU - Iacucci, Marietta
AU - Rescigno, Maria
AU - Di Sabatino, Antonio
N1 - Publisher Copyright:
© The Author(s) 2025.
PY - 2025/12
Y1 - 2025/12
N2 - Esophageal barrier has been investigated until now on the epithelial side only. Gut vascular barrier dysfunction has been recently implicated in a number of immune-mediated gastrointestinal disorders. We here characterized the esophageal vascular barrier (EVB) in eosinophilic esophagitis (EoE). Probe-based confocal laser endomicroscopy (pCLE) was performed in two EoE and two reflux esophagitis (RE) patients. The vascular barrier marker plasmalemma vesicle-1 (PV-1) was investigated as a measure of barrier disruption, by both immunohistochemistry and qPCR, in esophageal biopsies of 16 patients with EoE, 15 with RE, and 15 healthy controls (HC). In EoE, but not RE, pCLE revealed leakage of the EVB, which was restored in one patient after dupilumab treatment. PV-1 was significantly increased in EoE in comparison to RE and HC, both in terms of protein and transcript levels, supporting vascular leakage. EVB is disrupted in active EoE. Further studies are needed to understand the diagnostic and pathogenic implications of this finding.
AB - Esophageal barrier has been investigated until now on the epithelial side only. Gut vascular barrier dysfunction has been recently implicated in a number of immune-mediated gastrointestinal disorders. We here characterized the esophageal vascular barrier (EVB) in eosinophilic esophagitis (EoE). Probe-based confocal laser endomicroscopy (pCLE) was performed in two EoE and two reflux esophagitis (RE) patients. The vascular barrier marker plasmalemma vesicle-1 (PV-1) was investigated as a measure of barrier disruption, by both immunohistochemistry and qPCR, in esophageal biopsies of 16 patients with EoE, 15 with RE, and 15 healthy controls (HC). In EoE, but not RE, pCLE revealed leakage of the EVB, which was restored in one patient after dupilumab treatment. PV-1 was significantly increased in EoE in comparison to RE and HC, both in terms of protein and transcript levels, supporting vascular leakage. EVB is disrupted in active EoE. Further studies are needed to understand the diagnostic and pathogenic implications of this finding.
KW - Confocal laser endomicroscopy
KW - Dupilumab
KW - Esophageal mucosa
KW - Plasmalemma vesicle-1
KW - Reflux esophagitis
UR - https://www.scopus.com/pages/publications/105010687722
U2 - 10.1038/s41598-025-09680-x
DO - 10.1038/s41598-025-09680-x
M3 - Article
C2 - 40664773
AN - SCOPUS:105010687722
SN - 2045-2322
VL - 15
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 25600
ER -