TY - JOUR
T1 - Doxycycline and zinc loaded silica-nanofibrous polymers as biomaterials for bone regeneration
AU - Toledano, Manuel
AU - Toledano-Osorio, Manuel
AU - Osorio, Raquel
AU - Carrasco-Carmona, Álvaro
AU - Gutiérrez-Pérez, José Luis
AU - Gutiérrez-Corrales, Aida
AU - Serrera-Figallo, María Angeles
AU - Lynch, Christopher D.
AU - Torres-Lagares, Daniel
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Themaintargetofbone tissue engineeringis todesignbiomaterials that supportbone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with 5% wt of SiO2-nanoparticles (HOOC-Si-Membrane) were doped with zinc (Zn-HOOC-Si-Membrane) or doxycycline (Dox-HOOC-Si-Membrane). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and covered with the membranes. After six weeks, the bone architecture was evaluated with micro computed tomography. Three histological analyses were utilized to analyse bone regeneration, including von Kossa silver nitrate, toluidine blue and fluorescence. All membrane-treated defects exhibited higher number of osteocytes and bone perimeter than the control group without the membrane. Zn-HOOC-Si-Membranes induced higher new bone and osteoid area than those treated with HOOC-Si-Membranes, and control group, respectively. Zn-HOOC-Si-Membranes and Dox-HOOC-Si-Membranes attained the lowest ratioM1macrophages/M2 macrophages. Dox-HOOC-Si-Membranes caused the lowest number of osteoclasts, and bone density. At the trabecular new bone, Zn-HOOC-Si-Membranes produced the highest angiogenesis, bone thickness, connectivity, junctions and branches. Zn-HOOC-Si-Membranes enhanced biological activity, attained a balanced remodeling, and achieved the greatest regenerative efficiency after osteogenesis and angiogenesis assessments. The bone-integrated Zn-HOOC-Si-Membranes can be considered as bioactivemodulators provoking a M2 macrophages (pro-healing cells) increase, being a potential biomaterial for promoting bone repair.
AB - Themaintargetofbone tissue engineeringis todesignbiomaterials that supportbone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with 5% wt of SiO2-nanoparticles (HOOC-Si-Membrane) were doped with zinc (Zn-HOOC-Si-Membrane) or doxycycline (Dox-HOOC-Si-Membrane). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and covered with the membranes. After six weeks, the bone architecture was evaluated with micro computed tomography. Three histological analyses were utilized to analyse bone regeneration, including von Kossa silver nitrate, toluidine blue and fluorescence. All membrane-treated defects exhibited higher number of osteocytes and bone perimeter than the control group without the membrane. Zn-HOOC-Si-Membranes induced higher new bone and osteoid area than those treated with HOOC-Si-Membranes, and control group, respectively. Zn-HOOC-Si-Membranes and Dox-HOOC-Si-Membranes attained the lowest ratioM1macrophages/M2 macrophages. Dox-HOOC-Si-Membranes caused the lowest number of osteoclasts, and bone density. At the trabecular new bone, Zn-HOOC-Si-Membranes produced the highest angiogenesis, bone thickness, connectivity, junctions and branches. Zn-HOOC-Si-Membranes enhanced biological activity, attained a balanced remodeling, and achieved the greatest regenerative efficiency after osteogenesis and angiogenesis assessments. The bone-integrated Zn-HOOC-Si-Membranes can be considered as bioactivemodulators provoking a M2 macrophages (pro-healing cells) increase, being a potential biomaterial for promoting bone repair.
KW - Bone regeneration
KW - Cells
KW - Doxycycline
KW - Macrophage
KW - Non-resorbable polymer
KW - Silica
KW - Vascularization
KW - Zinc
UR - https://www.scopus.com/pages/publications/85086627333
U2 - 10.3390/POLYM12051201
DO - 10.3390/POLYM12051201
M3 - Article
AN - SCOPUS:85086627333
SN - 2073-4360
VL - 12
JO - Polymers
JF - Polymers
IS - 5
M1 - 1201
ER -