Effect of palm oil leaf extract eliais guinensis (standardized ethanolic fraction) on progression of experimental nephrotoxicity induced by gentamicin in sprague dawley rats

Recently, palm oil leaf extract is added as a health product in food industry due to its polyphenols and antioxidant vitamins contents. In the experiment, the in vitro antioxidant activity of the extract was measured using free radical scavenging activity, reducing power assay, hydrogen peroxide scavenging activity and ferric ion induced lipid peroxidation inhibition assay. Results revealed that the extract has an antioxidant activity. The renal-protective activity was studied using gentamicin model of experimental nephrotoxicity. It was assessed by measuring serum creatinine, creatinine clearance and absolute and fractional excretion of both sodium and potassium. Malonyldialdehyde (a biomarker of oxidative stress) was measured in renal homogenate. Six groups of rats were used, viz, C; control group, G group; injected with 80 mg/kg/day gentamicin sulphate intraperitoneally for 24 days, GP0.5, GP1 and GP2; fed with 0.5,1 and 2 gm/Kg (body weight)/ day of POLE orally along with gentamicin as mentioned above and P group; fed with highest dose of POLE. Results showed presence of renal-protective effect for the extract with the higher dose. It is ascribed to its content of polyphenols.