Epidermolytic toxin serotype B of Staphylococcus aureus is plasmid-encoded

Paul W. O'Toole; Timothy J. Foster

doi:10.1111/j.1574-6968.1986.tb01716.x

Epidermolytic toxin serotype B of Staphylococcus aureus is plasmid-encoded

Paul W. O'Toole
, Timothy J. Foster

Trinity College Dublin

Research output: Contribution to journal › Article › peer-review

Abstract

The gene coding for epidermolytic toxin serotype B (etb) was cloned in a plasmid expression vector in Escherichia coli. Its expression was dependent on the vector plasmid's trc promoter. A polypeptide immunochemically indistinguishable from the purified staphylococcal toxin and with the same molecular weight was predominantly localized in the periplasm of E. coli. The etb gene resides on a 1.7 kb HindIII fragment of the 42 kb plasmid pTC142 present in the parental Staphylococcus aureus strain.

Original language	English
Pages (from-to)	311-314
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	36
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1986.tb01716.x
Publication status	Published - Sep 1986
Externally published	Yes

Keywords

epidermolytic toxin serotype B
plasmid
Staphylococcus aureus
toxin

Access to Document

10.1111/j.1574-6968.1986.tb01716.x

Cite this

@article{009382efe2014c05a938213d39bc796a,

title = "Epidermolytic toxin serotype B of Staphylococcus aureus is plasmid-encoded",

abstract = "The gene coding for epidermolytic toxin serotype B (etb) was cloned in a plasmid expression vector in Escherichia coli. Its expression was dependent on the vector plasmid's trc promoter. A polypeptide immunochemically indistinguishable from the purified staphylococcal toxin and with the same molecular weight was predominantly localized in the periplasm of E. coli. The etb gene resides on a 1.7 kb HindIII fragment of the 42 kb plasmid pTC142 present in the parental Staphylococcus aureus strain.",

keywords = "epidermolytic toxin serotype B, plasmid, Staphylococcus aureus, toxin",

author = "O'Toole, \{Paul W.\} and Foster, \{Timothy J.\}",

year = "1986",

month = sep,

doi = "10.1111/j.1574-6968.1986.tb01716.x",

language = "English",

volume = "36",

pages = "311--314",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "2-3",

}

TY - JOUR

T1 - Epidermolytic toxin serotype B of Staphylococcus aureus is plasmid-encoded

AU - O'Toole, Paul W.

AU - Foster, Timothy J.

PY - 1986/9

Y1 - 1986/9

N2 - The gene coding for epidermolytic toxin serotype B (etb) was cloned in a plasmid expression vector in Escherichia coli. Its expression was dependent on the vector plasmid's trc promoter. A polypeptide immunochemically indistinguishable from the purified staphylococcal toxin and with the same molecular weight was predominantly localized in the periplasm of E. coli. The etb gene resides on a 1.7 kb HindIII fragment of the 42 kb plasmid pTC142 present in the parental Staphylococcus aureus strain.

AB - The gene coding for epidermolytic toxin serotype B (etb) was cloned in a plasmid expression vector in Escherichia coli. Its expression was dependent on the vector plasmid's trc promoter. A polypeptide immunochemically indistinguishable from the purified staphylococcal toxin and with the same molecular weight was predominantly localized in the periplasm of E. coli. The etb gene resides on a 1.7 kb HindIII fragment of the 42 kb plasmid pTC142 present in the parental Staphylococcus aureus strain.

KW - epidermolytic toxin serotype B

KW - plasmid

KW - Staphylococcus aureus

KW - toxin

UR - https://www.scopus.com/pages/publications/0022482841

U2 - 10.1111/j.1574-6968.1986.tb01716.x

DO - 10.1111/j.1574-6968.1986.tb01716.x

M3 - Article

AN - SCOPUS:0022482841

SN - 0378-1097

VL - 36

SP - 311

EP - 314

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

Epidermolytic toxin serotype B of Staphylococcus aureus is plasmid-encoded

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this