Establishment of mouse expanded potential stem cells

Jian Yang; David J. Ryan; Wei Wang; Jason Cheuk Ho Tsang; Guocheng Lan; Hideki Masaki; Xuefei Gao; Liliana Antunes; Yong Yu; Zhexin Zhu; Juexuan Wang; Aleksandra A. Kolodziejczyk; Lia S. Campos; Cui Wang; Fengtang Yang; Zhen Zhong; Beiyuan Fu; Melanie A. Eckersley-Maslin; Michael Woods; Yosuke Tanaka; Xi Chen; Adam C. Wilkinson; James Bussell; Jacqui White; Ramiro Ramirez-Solis; Wolf Reik; Berthold Göttgens; Sarah A. Teichmann; Patrick P.L. Tam; Hiromitsu Nakauchi; Xiangang Zou; Liming Lu; Pentao Liu

doi:10.1038/nature24052

Establishment of mouse expanded potential stem cells

Jian Yang
, David J. Ryan
, Wei Wang
, Jason Cheuk Ho Tsang
, Guocheng Lan
, Hideki Masaki
, Xuefei Gao
, Liliana Antunes
, Yong Yu
, Zhexin Zhu
, Juexuan Wang
, Aleksandra A. Kolodziejczyk
, Lia S. Campos
, Cui Wang
, Fengtang Yang
, Zhen Zhong
, Beiyuan Fu
, Melanie A. Eckersley-Maslin
, Michael Woods
, Yosuke Tanaka

Xi Chen, Adam C. Wilkinson, James Bussell, Jacqui White, Ramiro Ramirez-Solis, Wolf Reik, Berthold Göttgens, Sarah A. Teichmann, Patrick P.L. Tam, Hiromitsu Nakauchi, Xiangang Zou, Liming Lu, Pentao Liu

Research output: Contribution to journal › Article › peer-review

Abstract

Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.

Original language	English
Article number	24052
Journal	Nature
Volume	550
Issue number	7676
DOIs	https://doi.org/10.1038/nature24052
Publication status	Published - 19 Oct 2017
Externally published	Yes

Access to Document

10.1038/nature24052

Cite this

Yang, J., Ryan, D. J., Wang, W., Tsang, J. C. H., Lan, G., Masaki, H., Gao, X., Antunes, L., Yu, Y., Zhu, Z., Wang, J., Kolodziejczyk, A. A., Campos, L. S., Wang, C., Yang, F., Zhong, Z., Fu, B., Eckersley-Maslin, M. A., Woods, M., ... Liu, P. (2017). Establishment of mouse expanded potential stem cells. Nature, 550(7676), Article 24052. https://doi.org/10.1038/nature24052

@article{ac45c6eaac9945c8b6d4a1330ad34cb8,

title = "Establishment of mouse expanded potential stem cells",

abstract = "Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.",

author = "Jian Yang and Ryan, \{David J.\} and Wei Wang and Tsang, \{Jason Cheuk Ho\} and Guocheng Lan and Hideki Masaki and Xuefei Gao and Liliana Antunes and Yong Yu and Zhexin Zhu and Juexuan Wang and Kolodziejczyk, \{Aleksandra A.\} and Campos, \{Lia S.\} and Cui Wang and Fengtang Yang and Zhen Zhong and Beiyuan Fu and Eckersley-Maslin, \{Melanie A.\} and Michael Woods and Yosuke Tanaka and Xi Chen and Wilkinson, \{Adam C.\} and James Bussell and Jacqui White and Ramiro Ramirez-Solis and Wolf Reik and Berthold G{\"o}ttgens and Teichmann, \{Sarah A.\} and Tam, \{Patrick P.L.\} and Hiromitsu Nakauchi and Xiangang Zou and Liming Lu and Pentao Liu",

year = "2017",

month = oct,

day = "19",

doi = "10.1038/nature24052",

language = "English",

volume = "550",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Research",

number = "7676",

}

Yang, J, Ryan, DJ, Wang, W, Tsang, JCH, Lan, G, Masaki, H, Gao, X, Antunes, L, Yu, Y, Zhu, Z, Wang, J, Kolodziejczyk, AA, Campos, LS, Wang, C, Yang, F, Zhong, Z, Fu, B, Eckersley-Maslin, MA, Woods, M, Tanaka, Y, Chen, X, Wilkinson, AC, Bussell, J, White, J, Ramirez-Solis, R, Reik, W, Göttgens, B, Teichmann, SA, Tam, PPL, Nakauchi, H, Zou, X, Lu, L & Liu, P 2017, 'Establishment of mouse expanded potential stem cells', Nature, vol. 550, no. 7676, 24052. https://doi.org/10.1038/nature24052

TY - JOUR

T1 - Establishment of mouse expanded potential stem cells

AU - Yang, Jian

AU - Ryan, David J.

AU - Wang, Wei

AU - Tsang, Jason Cheuk Ho

AU - Lan, Guocheng

AU - Masaki, Hideki

AU - Gao, Xuefei

AU - Antunes, Liliana

AU - Yu, Yong

AU - Zhu, Zhexin

AU - Wang, Juexuan

AU - Kolodziejczyk, Aleksandra A.

AU - Campos, Lia S.

AU - Wang, Cui

AU - Yang, Fengtang

AU - Zhong, Zhen

AU - Fu, Beiyuan

AU - Eckersley-Maslin, Melanie A.

AU - Woods, Michael

AU - Tanaka, Yosuke

AU - Chen, Xi

AU - Wilkinson, Adam C.

AU - Bussell, James

AU - White, Jacqui

AU - Ramirez-Solis, Ramiro

AU - Reik, Wolf

AU - Göttgens, Berthold

AU - Teichmann, Sarah A.

AU - Tam, Patrick P.L.

AU - Nakauchi, Hiromitsu

AU - Zou, Xiangang

AU - Lu, Liming

AU - Liu, Pentao

PY - 2017/10/19

Y1 - 2017/10/19

N2 - Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.

AB - Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.

UR - https://www.scopus.com/pages/publications/85031943044

U2 - 10.1038/nature24052

DO - 10.1038/nature24052

M3 - Article

C2 - 29019987

AN - SCOPUS:85031943044

SN - 0028-0836

VL - 550

JO - Nature

JF - Nature

IS - 7676

M1 - 24052

ER -

Establishment of mouse expanded potential stem cells

Abstract

Access to Document

Other files and links

Fingerprint

Cite this