TY - JOUR
T1 - Evaluation of the phosphorescent palladium(II)-coproporphyrin labels in separation-free hybridization assays
AU - Burke, Martina
AU - O'Sullivan, Paul J.
AU - Soini, Aleksi E.
AU - Berney, Helen
AU - Papkovsky, Dmitri B.
PY - 2003/9/15
Y1 - 2003/9/15
N2 - Palladium(II)-coproporphyrin label and a set of corresponding monofunctional labeling reagents with different linker arms were evaluated for labeling of oligonucleotides and subsequent use in hybridization assays. The properties of resulting oligonucleotide probes including phosphorescence spectra, quantum yields, lifetimes, and labeling yields were examined as functions of the label and oligonucleotide structures. Upon hybridization with complementary sequences bearing dabcyl, QSY-7, and rhodamine green dyes, the probes displayed strong quenching due to close proximity effects. Intensity and lifetime changes of the phosphorescence, distance, and temperature dependences were investigated in detail. The potential of the new label and probes for sensitive and separation-free hybridization assays was discussed.
AB - Palladium(II)-coproporphyrin label and a set of corresponding monofunctional labeling reagents with different linker arms were evaluated for labeling of oligonucleotides and subsequent use in hybridization assays. The properties of resulting oligonucleotide probes including phosphorescence spectra, quantum yields, lifetimes, and labeling yields were examined as functions of the label and oligonucleotide structures. Upon hybridization with complementary sequences bearing dabcyl, QSY-7, and rhodamine green dyes, the probes displayed strong quenching due to close proximity effects. Intensity and lifetime changes of the phosphorescence, distance, and temperature dependences were investigated in detail. The potential of the new label and probes for sensitive and separation-free hybridization assays was discussed.
KW - DNA probes
KW - Hybridization assays
KW - Pd porphyrins
KW - Phosphorescence
KW - Proximity quenching
KW - Time-resolved fluorescence
UR - https://www.scopus.com/pages/publications/0042622450
U2 - 10.1016/S0003-2697(03)00383-X
DO - 10.1016/S0003-2697(03)00383-X
M3 - Article
C2 - 12927834
AN - SCOPUS:0042622450
SN - 0003-2697
VL - 320
SP - 273
EP - 280
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -